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1.
tific Experiment Center of Guangxi Medical University,Nanning 530021,China)Objective:To investigate the mutation pattern of adenomatous polyposis coli(APC),Kirsten-ras(K-ras) and p53 genes in sporadic colorectal cancer tissues.Meth 相似文献
2.
基于电化学阻抗技术构建了一种检测p53抑癌基因的电化学传感器.首先利用自组装作用将末端带巯基的探针p53抑癌基因(p53-DNA)固定于金电极表面,根据传感器结合前后电子转移阻抗值的变化,对目标p53-DNA进行了测定.以pH =7.4的5mmol/L K3[Fe(CN)6]-5mmol/L K4[Fe(CN)6]平衡电对溶液作为检测液,检测目标p53-DNA的线性浓度范围为1.0×10-8~ 1.0×10-6 mol/L,其检出限为3.0×10-9 mol/L(S/N =3).对5.0×10-8 mol/L的目标p53-DNA进行11次平行测定,其RSD为3.4%.该传感器制作简单、灵敏高、选择性好,且无需标记,易于操作. 相似文献
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Marco Riani 《Computational statistics & data analysis》2010,54(12):3300-3312
The forward search provides data-driven flexible trimming of a Cp statistic for the choice of regression models that reveals the effect of outliers on model selection. An informed robust model choice follows. Even in small samples, the statistic has a null distribution indistinguishable from an F distribution. Limits on acceptable values of the Cp statistic follow. Two examples of widely differing size are discussed. A powerful graphical tool is the generalized candlestick plot, which summarizes the information on all forward searches and on the choice of models. A comparison is made with the use of M-estimation in robust model choice. 相似文献
5.
Forced internal waves at the interface of a two-layer incompressible fluid in a two-dimensional domain with rigid horizontal boundaries are studied. The lower boundary is assumed to have a small obstruction. We derive a time-dependent forced modified KdV equation when the KdV theory fails and study the stabilities of four types of symmetric time-independent solitary-wave-like solutions numerically. 相似文献
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P53基因、Bcl-XL基因表达对淋巴性肿瘤细胞株辐射敏感性的影响 总被引:1,自引:0,他引:1
本文运用PCR-SSCP银染法检测人淋巴细胞恶性肿瘤株8226、U266、Raji、Jurkat、Daudi p53基因状态,RT-PCR半定量检测了Bcx-xL的mRNA的相对表达,DNA片段释放法检测细胞受照后24h的细胞DNA链断裂量,探讨了p53基因功能状态及Bcl-xL基因表达对细胞株8226、U266、Raji、Jurkat、Dsaudi辐射敏感性的影响。结果表明,淋巴细胞恶性肿瘤株8226、U266、Raji、Jurkat、Daudi均存在p53基因突变,高表达Bcl-xL基因的细胞株较低表达细胞株有较强的辐射耐性。 相似文献
8.
Zhenya Yan 《Computer Physics Communications》2003,152(1):1-8
In this paper based on a system of Riccati equations with variable coefficients, we present a new Riccati equation with variable coefficients expansion method and its algorithm, which are direct and more powerful than the tanh-function method, sine-cosine method, the generalized hyperbolic-function method and the generalized Riccati equation with constant coefficient expansion method to construct more new exact solutions of nonlinear differential equations in mathematical physics. A pair of generalized Hamiltonian equations is chosen to illustrate our algorithm such that more families of new exact solutions are obtained which contain soliton-like solution and periodic solutions. This algorithm can also be applied to other nonlinear differential equations. 相似文献
9.
电离辐射对EL-4细胞的P53蛋白及其下游基因MDM2 mRNA和蛋白表达的影响 总被引:8,自引:0,他引:8
采用流式细胞术和Northern blot杂交检测了X射线照射后EL-4细胞中p53蛋白及其下游基因MDM2mRNA和蛋白表达的变化,以探讨X射线照射诱导EL-4细胞G1期阻滞的分子机制。结果表明:经4GyX射线照射后的EL-4细胞,p53蛋白表达在照射后2h增高,4h达峰值,持续至照射后24h(p〈0.05~0.001);MDM2蛋白的表达在照射后4~24h明显升高(p〈0.05~0.01)。进 相似文献
10.
Svetlana A. Smirnikhina Milyausha I. Zaynitdinova Vasilina A. Sergeeva Alexander V. Lavrov 《International journal of molecular sciences》2022,23(11)
Genome editing is currently widely used in biomedical research; however, the use of this method in the clinic is still limited because of its low efficiency and possible side effects. Moreover, the correction of mutations that cause diseases in humans seems to be extremely important and promising. Numerous attempts to improve the efficiency of homology-directed repair-mediated correction of mutations in mammalian cells have focused on influencing the cell cycle. Homology-directed repair is known to occur only in the late S and G2 phases of the cell cycle, so researchers are looking for safe ways to enrich the cell culture with cells in these phases of the cell cycle. This review surveys the main approaches to influencing the cell cycle in genome editing experiments (predominantly using Cas9), for example, the use of cell cycle synchronizers, mitogens, substances that affect cyclin-dependent kinases, hypothermia, inhibition of p53, etc. Despite the fact that all these approaches have a reversible effect on the cell cycle, it is necessary to use them with caution, since cells during the arrest of the cell cycle can accumulate mutations, which can potentially lead to their malignant transformation. 相似文献