CTB-InsB融合基因在大肠杆菌中的表达研究

构建了一种霍乱毒素B亚基（CTB）与人胰岛素B链（InsB）的融合基因，并克隆入表达载体pGEX-4T-1中，命名为pGEX—CTB-InsB。该融合蛋白在大肠杆菌中的表达产物以包涵体形式存在，分子量约为43kDa。包涵体经溶解和复性后，使用GSTrapFF亲和层析柱纯化得到融合蛋白。经SDS-PAGE和Western免疫印迹分析证实纯化蛋白为重组目的蛋白。     

净化方法对1235铝合金热变形材料常数的影响

采用动态热模拟试验技术对经不同净化方法处理的1235铝合金进行热压缩变形试验,用多元回归法直接求解该合金的热变形材料常数,并重点分析夹杂物含量对热变形材料常数的影响规律.结果表明,净化处理效果对1235铝合金的热变形材料常数、尤其是热变形激活能有着显著影响,处理效果越好,热变形激活能(Q)就越低.经高效净化处理的1235铝合金的含杂量最低,Q值也最小(149.51 kJ/mol),具有很好的热塑性变形性能;含杂量与热变形激活能具有正的线性相关性,含杂量越低,热变形激活能也就越低.产生这种影响的原因主要在于氧化夹杂物(AI2O3等)在铝材热变形过程中对位错产生钉扎作用,限制了位错的束集,增大了扩展位错的宽度,使铝合金的层错能降低,位错不易产生交滑移,从而使其热变形激活能增大.     

Simultaneous process to isolate actomyosin and actin from post-rigor porcine skeletal muscle

A simultaneous actomyosin and actin isolation procedure from post-rigor porcine muscle was developed, based on differential solubility, gel filtration chromatography and extraction steps. The isolation process was evaluated by SDS-PAGE analysis and silver staining. Actomyosin and actin were isolated in a simultaneous process yielding 0.14 mg and 2.5 mg/g of meat, respectively, using a shorter purification process than others reported in the literature but with similar recoveries. Furthermore, actin preserves its polymerisation ability and both proteins, actomyosin and actin, could be used in further studies.     

室内空气污染因素对人体的危害及净化技术措施

介绍了室内主要污染因素即化学性、生物性、物理性因素对人体健康的危害及室内空气净化方法：吸附过滤净化技术、低温等离子体技术、臭氧净化技术、光催化技术。主要叙述了纳米光催化技术的原理、光催化剂的制备方法及纳米光催化技术在室内空气净化中的应用。     

聚丙烯工艺中原料丙烯的净化

分析了聚丙烯工艺中原料丙烯内多种杂质，诸如水、硫化物（COS、H2S）、氧、砷等对聚丙烯催化剂和产品质量的影响，指出聚丙烯原料净化的必要性和具体指标，阐述了多种净化过程，如脱水、COS水解、脱硫、脱氧、脱砷的反应机理、工业上常用净化剂的技术指标和使用条件。     

提纯建筑文化 走出“似”的误区

针对我国当今建筑市场上“似”的现象，提出提纯的概念，指出提纯与求似的根本区别，并结合具体设计实例总结提纯方法的客观依据，以期用提纯的设计方法，走出模仿的误区。     

Use of commercial protease preparations to reduce protein and lipid content of maize starch

A method was developed to produce pure maize starch from maize flour using a protease processing step, and additional salt washing and sulphite steeping steps. A range of commercial protease enzymes were evaluated for this purpose. The laboratory scale procedure that was developed, using one protease in particular (Promod P25P, thermolysin), produced relatively pure starch (<0.45% protein). Using the same procedure, but applying to starches which had been produced in advance using traditional wet milling, starch protein contents could be reduced further by 25–50% with the lipid content reduced by up to 25%. The amount of starch damage was minimal using this approach (<1%). This procedure could be developed industrially for a ‘greener approach’ to starch extraction – although it may still be necessary to incorporate sulphite steeping stages to facilitate protein solubilisation and extraction.     

Increase of trans-resveratrol in typical Sicilian wine using β-Glucosidase from various sources

β-Glucosidase (EC 3.2.1.21) (β-G) from different sources were tested to increase the trans-resveratrol in some Sicilian wines by hydrolysing resveratrol glucoside. β-G from Aspergillus niger mould was tested as a crude and purified enzyme, and compared with the same enzyme from Saccharomyces cerevisiae yeast. Specific purification served to eliminate collateral enzyme activities so that β-G could be used simply and economically. Aspergillus niger β-G produced trans-resveratrol increases of up to 75%, with no change in physico-chemical properties and bouquet, and an increase in health and nutritional properties. S. cerevisiae β-G raised free-terpenol levels, but impaired wine colour due to anthocyanase activity.     

稀土金属提纯方法与研究进展

对各种提纯稀土金属方法的机理进行了阐述,包括真空熔炼、电解精炼、真空蒸馏/升华、熔盐萃取、区域熔炼、固态电迁移、电化学脱氧、外吸气剂法、等离子体熔炼(通氢气或通氩气)等。对研究现状和提纯效果进行了总结。杂质去除需采用多种手段结合,同时提升装备水平,以达到提高提纯效果、降低成本、缩短生产周期的目的。