一类具有时滞的广义Hopf ield 神经网络的全局稳定性

通过引入能量泛函，分析了一类具有时滞的广义Hopfield神经网络的全局稳定性．从理论上给出了该类网络为全局稳定的充分条件，证明了当时滞满足一个可计算的边界条件时，具有时滞的该类神经网络与相应的无时滞网络具有同样的全局稳定特性．仿真结果进一步证明了结论的有效性。     

基于扩展k阶近邻法的电力系统稳定评估新算法

针对k阶近邻法分类时对样本的潜在结构信息未加利用这一缺陷,扩展k阶近邻法采用模式发现算法获取样本的空间分布知识,以获得的知识取代原始样本实现未知样本的分类。算法有效剔除了不利于分类的干扰样本,提高了分类精度和速度。在基于稳态运行信息的暂态稳定评估算法中,应用扩展k阶近邻法,实现了各种方式下稳定水平的正确判别。仿真结果验证了算法的有效性。算法作为一种通用的知识获取工具有广泛的应用前景。     

Improvement of the Energy Stability of the Single Ion Microbeam

Energy instability strongly affects the state and the beam size of the single ion microbeam. A facility based on the Generating Voltmeter was developed to improve the energy stability of the CAS-LIBB （Chinese Academy of Sciences, key laboratory of ion beam bioengineering） single ion microbeam. This paper presents the analysis of the energy＇ instability of the single ion microbeam. A simplified theoretical model is set up to calculate the relationship between the energy instability and the beam spot size. By using this technique, the energy instability is adjusted to about 1%. Stable run-time is over 6 hours. The radius of the single ion beam is reduced by 10% compared to the previous olin.     

高性能计算机电源系统的设计与实现

从系统级、机柜级、板级三方面详细介绍了某高性能计算机电源系统的实现方案。重点介绍了计算板的电源和控制电路的设计方法,并对电源系统的稳定性进行了分析,提出了解决方法,最后给出了系统运行时的电源实测波形。运行结果表明,该系统具有供电质量高,可靠性高,易于维护,能进行故障诊断等优点。     

静电感应晶闸管的I-V特性物理分析

考虑到寿命不等时的双注入效应、载流子寿命,特别是少子空穴寿命的变化、电导调制效应、双注入空间电荷限制效应等因素,对静电感应晶闸管(Static Induction Thyristor,简称SITH)在正向阻断态的整个I-V特性进行了分段物理分析,给出了理论解释并进行了计算,得出的结论与实验观测结果相吻合.     

BK Polyomavirus bkv-miR-B1-5p: A Stable Micro-RNA to Monitor Active Viral Replication after Kidney Transplantation

Background: Bkv-miR-B1-5p is a viral micro-RNA (miRNA) specifically produced during BK polyomavirus (BKPyV) replication. Recent studies have suggested using bkv-miR-B1-5p as a biomarker to monitor viral infection and predict complications in kidney transplant patients. To identify the technical limitations of this miRNA quantification in biological samples, knowledge of its stability and distribution in the extracellular compartment is necessary. Moreover, a proof of concept for using bkv-miR-B1-5p as a biomarker of active replication in chronic infection is still missing in the published literature. Methods: The stability of bkv-miR-B1-5p was evaluated in samples derived from cell cultures and in urine from BKPyV-infected kidney transplant recipients. The miRNA was quantified in different fractions of the extracellular compartment, including exosomes, and protein binding was evaluated. Finally, we developed an in vitro model for chronic culture of BKPyV clinical isolates to observe changes in the bkv-miR-B1-5p level during persistent infections. Results: Bkv-miR-B1-5p is a stable biomarker in samples from humans and in vitro experiments. Marginally associated with the exosomes, most of the circulating bkv-miR-B1-5p is bound to proteins, especially Ago2, so the miRNA quantification does not require specific exosome isolation. The bkv-miR-B1-5p level is predictable of viral infectivity, which makes it a potential specific biomarker of active BKPyV replication after kidney transplantation.     

Directed Evolution of Phi Class Glutathione Transferases Involved in Multiple-Herbicide Resistance of Grass Weeds and Crops

The extensive application of herbicides in crop cultivation has indisputably led to the emergence of weed populations characterized by multiple herbicide resistance (MHR). This phenomenon is associated with the enhanced metabolism and detoxifying ability of endogenous enzymes, such as phi class glutathione transferases (GSTFs). In the present work, a library of mutant GSTFs was created by in vitro directed evolution via DNA shuffling. Selected gstf genes from the weeds Alopecurus myosuroides and Lolium rigidum, and the cereal crops Triticum durum and Hordeum vulgare were recombined to forge a library of novel chimeric GSTFs. The library was activity screened and the best-performing enzyme variants were purified and characterized. The work allowed the identification of enzyme variants that exhibit an eight-fold improvement in their catalytic efficiency, higher thermal stability (8.3 °C) and three-times higher inhibition sensitivity towards the herbicide butachlor. The crystal structures of the best-performing enzyme variants were determined by X-ray crystallography. Structural analysis allowed the identification of specific structural elements that are responsible for k_cat regulation, thermal stability and inhibition potency. These improved novel enzymes hold the potential for utilization in biocatalysis and green biotechnology applications. The results of the present work contribute significantly to our knowledge of the structure and function of phi class plant GSTs and shed light on their involvement in the mechanisms of MHR.     

二阶方程稳定性的简易计算判别

很多实际问题都可归结为二阶周期性线性方程,这类方程的稳定性意味着所有解皆为周期解或拟周期解,于是初值如何都对应稳定的周期解或拟周期解。在应用上就说明该实际问题总有稳定运动状态。本文应用Hill方程的一个判别式给出了一个通过简单计算即可判定二阶方程稳定性的方法。数值例子说明该方法具有很好的实用性,同时有较高的精确度。     

Assessment of the Influence of the Selected Range of Visible Light Radiation on the Durability of the Gel with Ascorbic Acid and Its Derivative

(1) Background: Depending on the type of hydrophilic polymer used, different types of hydrogels may be chemically stable or may degrade and eventually disintegrate, or dissolve upon exposure to sunlight. Many over-the-counter medications are now stored with a limited control of temperature, humidity and lighting. Therefore, in this study, the photostability of a gel made of cross-linked polyacrylic acid (PA), methylcellulose (MC) and aristoflex (AV) was assessed, and the interaction between the polymers used and ascorbic acid and its ethylated derivative was investigated. (2) Methods: The samples were continuously irradiated at constant temperature for six hours. The stability of the substance incorporated into the gels was assessed using a UV-Vis spectrophotometer. FTIR-ATR infrared spectroscopy was used to measure changes during the exposure. (3) Results: Ascorbic acid completely decomposed between the first and second hours of illumination in all samples. The exception is the preparation based on polyacrylic acid with glycerol, in which the decomposition of ascorbic acid slowed down significantly. After six hours of irradiation, the ethylated ascorbic acid derivative decomposed in about 5% for the polyacrylic acid-based gels and aristoflex, and in the methylcellulose gel it decomposed to about 2%. In the case of ascorbic acid, the most stable formulation was a gel based on polyacrylic acid and polyacrylic acid with glycerol, and in the case of the ethyl derivative, a gel based on methylcellulose. (4) Conclusions: The experiment showed significant differences in the decomposition rate of both compounds, resulting from their photostability and the polymer used in the hydrogel.     

氢氧化钠/尿素/硫脲溶剂体系对纤维素溶解性能研究

通过设计正交实验,研究不同组成的氢氧化钠/尿素/硫脲溶剂体系对纤维素的溶解性能,确定了该溶剂体系中各组分的最佳含量,并通过X-射线衍射分析、红外光谱分析、热重分析等手段,表征了该溶剂体系获得的再生纤维素膜的结构和性能。结果表明:该溶剂体系对纤维素有良好的溶解性能,且溶解的纤维素再生后为纤维素Ⅱ,但其热稳定性低于原纤维素。