Nisin expression production from Lactococcus lactis in milk whey medium

BACKGROUND: Nisin is a commercially available bacteriocin produced by Lactococcus lactis ATCC 11454 and used as a natural agent in the biopreservation of food. In the current investigation, milk whey, a byproduct from dairy industries was used as a fermentation substrate for the production of nisin. Lactococcus lactis ATCC 11454 was developed in a rotary shaker (30 °C/36 h/100 rpm) using two different media with milk whey (i) without filtration, pH 6.8, adjusted with NaOH 2 mol L^?1 and without pH adjustment, both autoclaved at 121 °C for 30 min, and (ii) filtrated (1.20 µm and 0.22 µm membrane filter). These cultures were transferred five times using 5 mL aliquots of broth culture for every new volume of the respective media. RESULTS: The results showed that culture media composed of milk whey without filtration supplied L. lactis its adaptation needs better than filtrated milk whey. Nisin titers, in milk whey without filtration (pH adjusted), was 11120.13 mg L^?1 in the second transfer, and up to 1628‐fold higher than the filtrated milk whey, 6.83 mg.L^?1 obtained in the first^t transfer. CONCLUSIONS: Biological processing of milk byproducts (milk whey) can be considered a profitable alternative, generating high‐value bioproducts and contributing to decreasing river disposals by dairy industries. Copyright © 2008 Society of Chemical Industry     

Temporal and spatial aspects of an environmental stimulus influence the dynamics of behavioral regulation of the Aplysia siphon-withdrawal response.

Exposure to turbulence, an environmental stimulus, produces behavioral adaptation in the Aplysia siphon-withdrawal response (SWR). The authors show that the duration and spatial extent of turbulence influence adaptation recovery. In terms of duration, recovery in whole animals and reduced preparations (tail, siphon, and CNS) was more rapid after longer exposures to turbulence (10 min) than after briefer exposures (10 s-5 min). In terms of spatial extent, recovery in reduced preparations was more rapid after diffuse turbulence (tail and siphon together) compared with focal turbulence (siphon alone). Furthermore, spatial extent and duration interact: Duration regulates recovery only when turbulence is diffuse. Results suggest that SWR adaptation reflects a composite of cellular processes, including short-term synaptic enhancement in L30 inhibitory interneurons. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Streaming potential measurements on stainless steels surfaces: evidence of a gel-like layer at the steel/electrolyte interface

Electric charges at the surface of a passive stainless steel are generally considered as concentrated either in the passive film itself, or at the metal/passive film interface, or in the electrical double layer at the film solution interface. Rest potential time dependence after immersion of a passive surface in aqueous electrolytes suggests however that slow processes occur in the onset of the surface charge. Specific experiments, such as streaming potential measurements and electrochemical impedance spectroscopy in a thin electrolyte cell, were carried out for understanding better this phenomenon. An AISI 304 type austenitic stainless steel with polished or bright annealed surface finishes was immersed in NaCl aqueous solutions with various pH and chloride concentrations. The streaming potential time evolution shows two steps: a first rapid one (∼2 min) is attributed to the onset of the surface charge. The second step is much slower (approximately several hours) and possibly due to an interphase layer between the passive film and the solution. Following this idea, the whole kinetics is controlled by cation migration across the interphase when the pH is larger than the isoelectric pH (pH_iep), while chloride ions are incorporated in the interphase when pH < pH_iep. Impedance measurements allow determining both the kinetics of charge transport and the thin cell conductivity. When glass is used as reference material for the cell walls instead of stainless steel, the Nyquist plots show a high-frequency response. For stainless steel cell walls, a low-frequency response is observed, attributed to a slow charge reorganisation inside the interphase layer. The charge distribution at metal/electrolyte interface is discussed in terms of a gel-like layer which possibly takes place at the passive film/electrolyte interface.     

高分辨率测井约束地震反演技术在本布图地区的应用

简要介绍了高分辨率测井约束地震反演技术应遵循的原则及实现步骤,并应用该技术对焉耆盆地本布图背斜构造带进行预测和分析。通过对比分析,反演剖面的分辨率和信噪比有所提高,能较好的反映储层性质的横向变化,预测结果与实际基本符合,说明高分辨率测井约束反演技术在该地区具有一定的实用性。     

Subspace distance analysis with application to adaptive Bayesian algorithm for face recognition

We propose subspace distance measures to analyze the similarity between intrapersonal face subspaces, which characterize the variations between face images of the same individual. We call the conventional intrapersonal subspace average intrapersonal subspace (AIS) because the image differences often come from a large number of persons. An intrapersonal subspace is referred to as specific intrapersonal subspace (SIS) if the image differences are from just one person. We demonstrate that SIS varies significantly from person to person, and most SISs are not similar to AIS. Based on these observations, we introduce the maximum a posteriori (MAP) adaptation to the problem of SIS estimation, and apply it to the Bayesian face recognition algorithm. Experimental results show that the adaptive Bayesian algorithm outperforms the non-adaptive Bayesian algorithm as well as Eigenface and Fisherface methods if a small number of adaptation images are available.     

FeSiB薄膜的巨磁阻抗和应力阻抗效应研究

软磁材料中存在巨磁阻抗 (giantmagneto impedance ,GMI)效应以及与之相同来源的应力阻抗 (stress impedance ,SI)效应 ,利用这两种效应可以制成具有高灵敏度的微型化的磁场和应力 应变传感器。本文基于传感器的实际应用 ,对图形化的、较大磁致伸缩的FeSiB单层和多层薄膜的巨磁阻抗和应力阻抗效应中频率和退火的影响进行了研究。结果表明 ,对于两种效应 ,经过退火处理的单层和多层膜均可在较低的频率下得到较高的灵敏度 ,而多层膜中的应力阻抗效应将为新型高灵敏传感器的设计和研制开辟一条崭新的途径     

用载文南定理计算感应电机和变压器的特性曲线

感应电机和变压器激磁电路的参数是非线性的.为准确求取它们的特性曲线,需要的计算量很大.应用本文引入的方法可以避免大的计算量又可得到准确的特性曲线.     

An integer concave minimization approach for the minimum concave cost capacitated flow problem on networks

Formulating the minimum concave cost capacitated network flow problem as an integer concave minimization problem, we establish finite branch and bound algorithms, in which the branching operation is the so–called integral rectangular partition and the bounding procedure is performed by the classical minimum linear cost flow problem on subnetworks. For the special case that the flow cost function is concave on a fixed number of arcs and linear on the others, an upper bound of the running time is given. Received: 19 July 1996 / Accepted: 8 July 1997     

认知定位系统的自适应精度研究

为了能够实现定位精度的自适应,本文分析了认知无线电定位系统(cognitive positioning systems,CPS)的原理。该系统利用频谱及环境感知等模块中的传感器单元来获取所需的定位参数,并将其与到达时间(time of arrival,TOA)定位估计算法相结合,以克拉美罗下界(Cramer-Rao lower bound,CRLB)为准则分析最大似然(maximum likelihood,ML)定位精度自适应算法。本文还对单径和多径环境条件下定位精度自适应性能进行了比较。仿真结果表明,该算法能够实现定位精度的自适应,并能通过改善系统可用的频带带宽、分散频谱的数目、信号的调制方式等因素来提高定位精度。     

行为优化提升软件配置管理

软件配置管理包括对代码、文档、数据等的管理,其优劣受限于项目成员的实际操作。开发人员对于工作区如何使用;成员之间的代码是不是可以及时更新与同步;怎样使用分支,如何进行变更合并,才能减少物理空间浪费和事件延迟。这些问题在实际的项目开发中往往被忽视,亦或团队并没有对成员行为作细节的规范,因而许多软件项目出现了工期推迟或代码质量不高等问题。为此提出了一系列管理措施,通过优化软件配置管理规范项目各成员的行为,以保证高效的软件配置管理的实施。