Yeast mutants with increased bacterial transposon Tn5 excision

Five complementing recessive mutations that exhibit increased bacterial transposon Tn5 precise excision in yeast Saccharomyces cerevisiae were obtained by ethylmethanesulfonate treatment. One of these mutations (tex1) was submitted to extensive genetic analysis. tex1 is a recessive temperature-sensitive mutation resulting in a 20-100-fold increase in Tn5 excision. It also has increased frequencies of ochre mutation reversion, of forward mutation to canavanine resistance, and loss of chromosome III or its right arm. The possible mechanism of tex1 effects is discussed.     

筛查基因突变的新技术——循环双脱氧指纹图谱法及其应用

建立了适用于大批量标本基因突变筛查的双脱氧指纹图谱法即ｄｄＦ。该技术民测定ＤＮＡ序列的双脱氧末端终止法及ＳＳＣＰ二者长处于一身，即只作一个双脱氧末端终止反应，然后将反应产物在与ＳＳＣＰ相同的电泳条件下进行聚丙烯酰胺凝胶电泳，这样有突变的样品不会漏检，阳性检出率高达１００％。     

基于基因表达式的克隆选择算法求解JSP问题

针对作业车间调度问题的特征,提出一种基于基因表达式的克隆选择算法。在这个方法中,采用基因表达式编程算法中的编码方式来表示调度方案,同时为了提出的方法具有更强的全局搜索能力,运用克隆选择算法作为搜索引擎。最后,验证提出的方法的有效性,对7组Benchmark实例进行测试。实验结果表明,基于基因表达式的克隆选择算法在求解作业车间调度问题中是非常有效的。     

机器人足球比赛系统中的机器人任务分配方法

针对基于遗传算法的多机器人任务分配方法中,由于初始种群是随机产生不能很好地表征整个解空间,而容易陷入局部最优解的问题,提出一种新的多机器人任务分配方法。该方法根据机器人效用函数值来确定个体基因,从而产生初始种群,并引进分层遗传算法来实现具有不同最优基因的群体分开演化。仿真实验表明,该方法比传统的遗传算法有更高的寻优效率和更快的收敛速度。     

Functionalized Reduced Graphene Oxide as a Versatile Tool for Cancer Therapy

Cancer is one of the deadliest diseases in human history with extremely poor prognosis. Although many traditional therapeutic modalities—such as surgery, chemotherapy, and radiation therapy—have proved to be successful in inhibiting the growth of tumor cells, their side effects may vastly limited the actual benefits and patient acceptance. In this context, a nanomedicine approach for cancer therapy using functionalized nanomaterial has been gaining ground recently. Considering the ability to carry various anticancer drugs and to act as a photothermal agent, the use of carbon-based nanomaterials for cancer therapy has advanced rapidly. Within those nanomaterials, reduced graphene oxide (rGO), a graphene family 2D carbon nanomaterial, emerged as a good candidate for cancer photothermal therapy due to its excellent photothermal conversion in the near infrared range, large specific surface area for drug loading, as well as functional groups for functionalization with molecules such as photosensitizers, siRNA, ligands, etc. By unique design, multifunctional nanosystems could be designed based on rGO, which are endowed with promising temperature/pH-dependent drug/gene delivery abilities for multimodal cancer therapy. This could be further augmented by additional advantages offered by functionalized rGO, such as high biocompatibility, targeted delivery, and enhanced photothermal effects. Herewith, we first provide an overview of the most effective reducing agents for rGO synthesis via chemical reduction. This was followed by in-depth review of application of functionalized rGO in different cancer treatment modalities such as chemotherapy, photothermal therapy and/or photodynamic therapy, gene therapy, chemotherapy/phototherapy, and photothermal/immunotherapy.