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81.
Fingerprinting G-protein-coupled receptors 总被引:6,自引:0,他引:6
Recently we reported the design of a discriminating fingerprintfor rhodopsin-like G-protein-coupled receptors (GPCRs). Thefingerprint encodes the seven putative membrane-spanning motifsand was potently diagnostic of all GPCRs (52 in all) in version8.1 of the OWL composite sequence database, readily distinguishingthem from all other integral membrane proteins. With a 3-foldincrease in the size of OWL, the fingerprint has been updatedand now finds 332 receptors that match all the motifs. The situation,however, has grown in complexity: 61 sequences make imperfectmatches with the fingerprint, yielding a total of 393 hits.The bulk of the partial hits are olfactory receptors: theseappear to fall into discrete subfamilies in which one or moreof the transmembrane motifs are either poorly matched or arenot matched at all. These results are supported by preliminaryphylogenetic analyses, which show the olfactory and variousother partial matches clustering away from the main body oftrue hits. The approach has provided a powerful diagnostic toolfor identifying GPCRs, and results are consistent with previousobservations that the pheromone, cAMP and secretin-like receptorsbelong to separate families-these bear their own unique sequencefingerprints by which they may be distinguished from the rhodopsin-likesuperfamily 相似文献
82.
中国狂犬病毒疫苗株CTN-1-V三个代次的GP基因序列分析 总被引:1,自引:2,他引:1
目的分析三个代次CTN-1-V病毒株糖蛋白(GP)基因部分序列,并与代表性街毒株进行比较。方法利用RTPCR反应,从感染了CTN-1-V病毒的小鼠脑内获得GPcDNA的部分片段,并进行序列测定。结果三个代次的CTN-1-V病毒株GPcDNA序列的690个核苷酸与代表性狂犬病毒GP核苷酸相应序列同源性为83.2%~96.8%,氨基酸序列同源性为90.0%~97.4%。CTN1V株三代之间的核苷酸序列几乎相同。结论CTN1V株在传代过程中GP基因结构基本稳定,与中国流行的代表性街毒株的同源性高于aG株和PV株。 相似文献
83.
本文研究了炭黑-环氧树脂复合材料的制备工艺及炭黑填充率对材料电阻率的影响。实验结果表明,加入表面活性剂,明显改善了炭黑在环氧树脂中分布的均匀性。炭黑-环氧树脂材料具有明显的渗流效应,正温度系数效应即PTC效应,非线性伏安特性和电磁波屏蔽效应。 相似文献
84.
The electrochemical behavior of the bronze (Cu-8Sn in wt%) was investigated in 3% NaCl aqueous solution, in presence and in absence of a corrosion inhibitor, the 3-phenyl-1,2,4-triazole-5-thione (PTS). The inhibiting effect of the PTS was evidenced for concentrations higher than 1 mM for the cathodic process whereas its effect was clearly seen with a concentration as low as 0.1 mM for the anodic process. A significant positive shift of the corrosion potential was also observed, and its inhibiting effect increased with both its concentration and the immersion time of the sample. From voltammetry and electrochemical impedance spectroscopy experiments, the inhibiting efficiency of the PTS was found to be in the 94-99% range for 1 mM concentration. Scanning electron microscopy and X-ray energy dispersion analysis of the specimen surface show the presence of sulphur on the surface. Raman micro-spectrometry study confirms the protective effect of the PTS in aqueous solution through three types of interactions with the electrode, namely the adsorption of the inhibitor in a flat configuration, the formation of copper-thiol molecules, and when copper is released, the formation of a polymeric complex. 相似文献
85.
以Al_2O_3、PdCl_2和RuCl_3为原料,采用水溶液浸渍法,通过控制固载顺序、制备流程以及焙烧温度等条件,制得系列负载型Ru Pd双金属催化剂,并用于对苯二甲酸二甲酯(DMT)制取1,4-环己烷二甲酸二甲酯(DMCD)的选择性加氢过程。其中,Ru和Pd的总负载量为0.3%(以催化剂总质量为基准,下同),且m(Ru)∶m(Pd)=1∶1。结果发现,采用先Ru后Pd(Ru-Pd)式固载顺序和浸渍-干燥-浸渍-干燥-焙烧(IDIDC)型制备流程,并在450℃下焙烧后,所得负载型Ru-Pd双金属催化剂的反应性能最佳,在6 MPa、180℃下,DMT转化率为89.6%,DMCD选择性为96.0%,DMCD产率为85.9%。这可能与Ru-Pd中大粒径粒子的形成受到抑制、粒径尺寸和分布更小、比表面积和总孔容更高、表面Pd原子摩尔分数较高以及Ru/Pd物质的量比较低有关。 相似文献
86.
In this paper, a nonlinear model reference adaptive impedance controller is proposed and tested. The controller provides asymptotic tracking of a reference impedance model for the robot end-effector in Cartesian coordinates applicable to rehabilitation robotics or any other human–robot interactions such as haptic systems. The controller uses the parameters of a desired stable reference model which is the target impedance for the robot’s end-effector. It also considers uncertainties in the model parameters of the robot. The asymptotic tracking is proven using Lyapunov stability theorem. Moreover, the adaptation law is proposed in joint space for reducing the complexity of its calculations; however, the controller and the stability proof are all presented in Cartesian coordinates. Using simulations and experiments on a two DOFs robot, the effectiveness of the proposed controller is investigated. 相似文献
87.
丁明华 《化工自动化及仪表》2003,30(4):49-50
介绍一种对电除尘工序进行远程自动控制的方法。使用DCS顺序控制图,通过模块组态和内部编程,实现多台设备的自动控制,优化工艺过程,提高该工序的自动化水平。 相似文献
88.
为了提高彩色图像加密的安全性和加密性能,设计了一种基于多混沌系统的彩色图像加密方法.将一个彩色图像分解为R、G、B三个灰度图像,使用MD5算法动态生成加密算法的初始值,然后使用三种不同的基于混沌的加密结构对三个图像进行加密.对R图像使用Feistel结构加密,其中Feistel结构的S一盒由Logistic混沌序列和Hyperhenon混沌序列组合产生;对G图像使用由Lorenz系统产生加密序列对图像进行代替和置换操作的加密结构;对B图像使用由分段线性混沌映射产生加密序列,然后加密图像的加密结构,再把加密后的图像结合起来生成加密后的图像.理论分析和实验结果表明,该加密方法能够较为有效地保证彩色图像加密的安全性. 相似文献
89.
Epoxy resin is chosen for our present study owing to its exceptional combination of properties such as easy processing, high safety, excellent solvent and chemical resistance, toughness, low shrinkage on cure, good electrical, mechanical and corrosion resistance with excellent adhesion to many substrates. This versatility in formulation made epoxy resins widely applied for surface coatings, adhesives, laminates, composites, potting, painting materials, encapsulant for semiconductor and insulating material for electric devices. There are numerous paint/coating systems based on epoxy resin available for corrosion and fouling prevention. They however are not completely satisfactory in field applications, where high corrosion, fouling and flame resistance are required. The demand for epoxy resin as corrosion/fouling resistant coatings is restricted mainly due to its inferior characteristics like poor impact strength, high rigidity, and moisture absorbing nature besides inadequate flame retardant properties. It is for this reason that silicones and phosphorus-based compounds are used as modifier in this work by intercrosslinking network mechanism (ICN) to obtain epoxy resin with desired properties ideally suitable for field applications for preventing corrosion and fouling with flame retardantancy. The present work involves the development of solvent free silicone/phosphorus modified epoxy coating systems, since solvent free coating systems are widely used for numerous applications due to their lower cost per unit film thickness, freedom from fire and pollution hazard and ability to provide better performance. For the development of coating systems, epoxy resin (X) serves as base material, hydroxyl terminated polydimethylsiloxane (HTPDMS) as modifier, γ-aminopropyltriethoxysilane (γ-APS) as crosslinking agent and dibutyltindilaurate (DBTDL) as catalyst. Polyamidoamine (A), aromatic amine adducts (B) and phosphorus-containing diamine (C) were used as curing agents. The study also describes the evaluation of corrosion resistant behaviour of unmodified epoxy and siliconized epoxy coatings by potentiodynamic polarization method, electrochemical impedance spectroscopy (EIS), salt-spray and antifouling tests. The results are discussed. 相似文献
90.
This work provides kinetic and transport parameters of Li-ion during its extraction/insertion into thin film LiNi0.5Mn1.5O4 free of binder and conductive additive. Thin films of LiNi0.5Mn1.5O4 (0.2 μm thick) were prepared on electronically conductive gold substrate utilizing the electrostatic spray deposition technique. High purity LiNi0.5Mn1.5O4 thin film electrodes were observed with cyclic voltammetry, to exhibit very sharp peaks, high reversibility, and absence of the 4 V signal related to the Mn3+/Mn4+ redox couple. The electrode subjected to 100 CV cycles of charge/discharge delivered a capacity of 155 mAh g−1 on the first cycle and sustained a good cycling behavior while retaining 91% of the initial capacity after 50 cycles. Kinetics and mass-transport of Li-ion extraction at LiNi0.5Mn1.5O4 thin film electrode were investigated by means of electrochemical impedance spectroscopy. The apparent chemical diffusion coefficient (Dapp) value determined from EIS measurements changed depending on the electrode potential in the range of 10−10-10−12 cm2 s−1. The Dapp profile shows two minimums at the potential values close to the peak potentials of the corresponding cyclic voltammogram. 相似文献