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41.
荧光法研究3种黄酮化合物与牛血清白蛋白的结合作用 总被引:2,自引:1,他引:1
运用荧光光谱法研究了生理酸度条件下(pH=7.4)3种黄酮化合物白杨素、芹菜素和桑色素与牛血清白蛋白(BSA)的结合作用。结果表明,3种黄酮化合物均能与BSA形成基态复合物导致BSA内源荧光猝灭,猝灭机理为静态猝灭。结合常数K(芹菜素)〉K(白杨素)〉K(桑色素),结合位点数近似等于1,白杨素与BSA之间以疏水作用为主,而芹菜素、桑色素与BSA之间的作用力主要是氢键和范德华力。黄酮分子结构中羟基位置及个数影响其与BSA间的结合作用,C4'-OH对结合有促进作用,而C3-OH的取代则导致作用力减弱。由Frster非辐射能量转移理论,计算出3种黄酮化合物在蛋白质中的结合位置与212位色氨酸残基间的距离分别为2.44 nm(白杨素)、3.34 nm(芹菜素)和3.31 nm(桑色素)。 相似文献
42.
铜离子是造成环境污染的重金属之一,如何检测出超低浓度的铜离子是人们一直关心的课题。合成了一种新型的纳米粒子荧光探针,利用荧光光谱法比较了Cd2+、Co2+、Ni2+、Pb2+、Mg2+、Zn2+、Ca2+、Cu2+对该纳米粒子荧光探针的荧光淬灭效应。结果表明,该纳米粒子荧光探针对Cu2+具有很敏感的检测作用,其检测浓度达到1.0×10-11mol/L。 相似文献
43.
Thermal activation and radiation quenching characteristics and their application to the pre-dose dating of porcelain 总被引:1,自引:1,他引:0
WeiDa Wang 《中国科学E辑(英文版)》2009,52(2):324-331
The pre-dose technique is important for thermoluminescence (TL) dating of porcelain. The principle of pre-dose dating is based on two characteristics: thermal activation and radiation quenching of the porcelain. Based on these principles, two measurement methods, "the activation method" and "the quenching method", respectively, have been developed for evaluation of the porcelain paleodose. The paleodose values obtained are the same for the ages in the range of 100―1000 a BP for porcelains measured by these two methods. But for dating at lower age limits (less than 100 a BP), the activation method is more accurate; conversely, at higher age limits (greater than 1000 a BP) the quenching method is more accurate. In addition, two specific calculating methods are described for a few porce-lains having anomalous activation and quenching characteristics that make these two methods invalid. 相似文献
44.
对大型42 CrMoS4圆钢锻材淬火出现粗晶、魏氏组织的原因进行分析表明,冷却方式及淬火介质是产生组织缺陷的主要原因。优化淬火工艺可以有效改善组织、细化晶粒和提高力学性能,满足生产技术要求。 相似文献
45.
对长轴类零件采用大功率中频机同时加热表面淬火的方法,配以专用感应圈和伸缩应圈的应用和自回火的应用,结果表明,可提高零件中频表面淬火质量,并大大提高生产效率,从而使企业在生产中取得高产品质量,高生产效率和低生产成本的极佳效果。 相似文献
46.
根据量化控制淬火原理,针对水基淬火介质,选用三个量化控制参数:K、Ts和Q,设计了符合量化淬火要求的循环冷却系统。 相似文献
47.
Evaluation of Process Capability in Gas Carburizing Process to Achieve Quality through Limit Design Concept 总被引:1,自引:0,他引:1
NUMEROUS INDUSTRIAL APPLICATIONS requirea hard wear-resistant surface called the case,and arelatively soft,tough inside called the core.There arefive principal methods of case hardening:?Carburizing?Nitriding?Cyaniding or carbonitriding?Flame hardening?Induction hardeningThe first three methods change the chemicalcomposition,carburizing by the addition of carbon,nitriding by the addition of nitrogen and cyaniding bythe addition of both carbon and nitrogen.The last twomethods do n… 相似文献
48.
KarlRitter StefanWiebach 《材料热处理学报》2004,25(5):607-611
IN the past five years the process combination of vacuum hardening, respectively vacuum carburizing with high-pressure gas quenching was successfully introduced to the market, especially in the manufacture of gears. In the meantime furnace concepts for various applications are available to the industry. In the following report three plant varieties are introduced, which differ in process flexibility and throughput. This report also explains criteria for the selection of a furnace in view of the existing application requirements. Besides this a short introduction is given into the vacuum carburizing process and the high-pressure gas quenching technology. 相似文献
49.
DESPITE of the rapid development of computerfacilities and numerical methods of computing thestress-strain state of large-size parts,the accuracy ofcomputations is not sufficient because of the lack ofaccurate input data.For this reason,heat treatingengineers do not trust computations and are afraid ofquenching of large-size parts in water,in particular,when Bi—>°°.There are some causes of suchsituation:1)Because of inaccurate boundary conditions andincomplete information on mechanical p… 相似文献
50.
A. Irretier O. Kessler F. Hoffmann P. Mayr 《Journal of Materials Engineering and Performance》2004,13(5):530-536
When precipitation-hardenable aluminum parts are water quenched, distortion occurs due to thermal stresses. Thereby, a costly
reworking is necessary, and for this reason polymer quenchants are often used to reduce distortion, with the disadvantage
that the quenched parts have to be cleaned after quenching. In opposition to liquid quenchants, gas quenching may decrease
distortion due to the better temperature uniformity during quenching. Furthermore, cleaning of the quenched parts can be avoided
because it is a dry process. For this purpose, a heat-treating process was evaluated that included a high-pressure gasquenching
step. Gas quenching was applied to different aluminum alloys (i.e., 2024, 6013, 7075, and A357.0), and tensile tests have
been carried out to determine the mechanical properties after solution annealing, gas quenching, and aging. Besides high-pressure
gas quenching, alloy 2024 was quenched at ambient pressure in a gas nozzle field. The high velocity at the gas outlet leads
to an accelerated cooling of the aluminum alloy in this case. Aluminum castings and forgings can be classified as an interesting
field of application of these quenching methods due to their near-net shape before the heat treatment. Cost savings would
be possible due to the reduced distortion, and therefore, less reworking after the precipitation hardening. 相似文献