厌氧活性污泥PCR-DGGE图谱的优化

针对特种污泥PCR-DGGE图谱的优化一直是微生物分子生态学研究的重点.在反硝化抑制硫酸盐还原菌的研究中,研究厌氧硫酸盐还原污泥和反硝化污泥DNA的提取方法,应用通用引物958F/1401R扩增16S rDNA序列,探讨不同的退火温度对DGGE指纹图谱多样性影响,同时采用时间进程法对DGGE图谱进行优化.结果表明,采用PBS洗涤污泥和超声震荡有利于硫酸盐还原污泥DNA提取;采用958F/1401R为引物时,扩增片段大约500 bp,不同的退火温度对DGGE指纹图谱的多样性影响不显著;采用时间进程法可以很好地再现不同电泳阶段对样品的分离效果,最佳变性剂梯度为40%～60%,在130 V电压下,最佳电泳时间为5 h.     

混凝土底板温度及温度应力影响参数分析

借助参数化设计语言(APDL)分析了水泥品种及标号、结构尺寸、外界约束对混凝土底板温度及温度应力的影响.结果表明:与普通硅酸盐水泥525相比,普通硅酸盐水泥425、矿渣水泥425可使底板最大拉应力分别减低约21%、37%;底板平面尺寸增大,压应力几乎保持不变,拉应力增大,发展较为平缓;底板厚度越大,出现最高温度的龄期越晚,底板降温时持续高温时间更长,且温度应力随厚度增大而增大.     

Evolution of carbides and carbon content in matrix of an ultra-high carbon sintered steel during heat treatment process

DTA, thermal expansion, XRD, and SEM were used to evaluate the effect of quenching temperature on the mechanical properties and microstructure of a novel sintered steel Fe-6Co-1Ni-5Cr-5Mo-1C. Lattice parameters and the mass fraction of carbon dissolved in the matrix of the steel quenched were investigated. It is discovered that the hardness of the steel increases with quench-ing temperature in the range of 840-900℃ and remains constant in the range of 900 to 1100℃. It decreases rapidly when the tem-perature is higher than 1100℃. The mass fraction of carbon dissolved in the matrix of the steel quenched at 840℃ is 0.38, but when the quenching temperature is increased to 1150℃, it increases to 0.98. The carbides formed during sintering are still present at grain boundaries and in the matrix of the steel quenched at low quenching temperatures, such as 840℃. When the quenching temperature is increased to 1150℃, most of the carbides at grain boundaries are dissolved with just a small amount of spherical M23C6 existing in the matrix of the quenched steel.     

Numerical simulation on the rheo-diecasting of the semi-solid A356 aluminum alloy

The effect of injection pressure, piston velocity, and the forming temperature of semisolid slurry on the filling behavior of the semi-solid A356 aluminum alloy was investigated by simulation methods. The simulation results show that these processing parameters have an important effect on the filling behavior of the semi-solid A356 aluminum alloy. The slurry flows steadily in the cavity when the injection pressure, the piston velocity, and the forming temperature are low, but it is prone to turbulent flow w...     

Effects of annealing temperature on the microstructure and properties of the 25Mn-3Si-3Al TWIP steel

Microstructures and mechanical properties of the 25Mn twinning induced plasticity (TWIP) steel at different annealing temperatures were investigated. The results indicated that when the annealing temperature was 1000°C,the 25Mn steel showed ex-cellent comprehensive mechanical properties,the tensile strength was about 640 MPa,the yield strength was higher than 255 MPa,and the elongation was above 82%. The microstructure was analyzed by optical microscopy (OM),X-ray diffraction (XRD),and transmission electron...     

F68大单体/HEMA共聚水凝胶的制备及性能研究(Ⅰ):温度敏感性

本文以温度敏感性Pluronic F68与甲基丙烯酸缩水甘油酯(GMA)反应制备可光聚合的F68/GMA大分子单体,再在水溶液中与丙烯酸(AA)单体和甲基丙烯酸β-羟乙酯(HEMA)经紫外光原位聚合制备温度敏感水凝胶。详细地研究了水凝胶在不同温度下的溶胀性能。溶胀动力学测量的结果表明,该水凝胶具有温度响应性且具有可逆性。     

Temperature distribution and dynamic control of secondary cooling in slab continuous casting

To predict and optimize the temperature distribution of slab continuous casting in steady operational state, a three-dimensional model (named offline model) based on the heat transfer and solidification theories was developed. Both heat transfer and flux distribution characteristics of the nozzle sprays on the slab were considered, and the complicated boundary condi-tions, such as spray cooling, natural convection, thermal radiation as well as contact cooling of individual rolls were involved in the model. ...     

Corner forming of AZ61A magnesium alloy tube within warm hydroforming

The corners with small radii on cross sections are crucial for forming hydroformed components with polygonal sections.In this paper,warm hydroforming experiments of AZ61A magnesium alloy tubes were carried out to study the forming regularity of round corners by using a demonstration part with square sections.Effects of temperature on radius forming,thinning ratio distribution and microstructure were revealed and a component with relative outer corner radius of 3.0 was obtained by warm hydroforming at 240℃.The minimum thickness of the formed square section was located in the transition position between the corner and the straight wall.The thinning ratio of the round corner increased with the increase of forming temperature.Formability of the magnesium tube was improved by raising temperature under the effect of dynamic recrystallization at 240℃.     

渣油加氢结焦反应条件的研究

使用高压釜反应器,无催化剂条件下,考察了反应温度(410～440 ℃)、初始氢气压力(3.0～7.0 MPa)及停留时间(20～80 min)对渣油加氢结焦及转化率的影响.当温度、停留时间增加时,渣油转化率与结焦率都增大,而初始氢气压力增加时渣油转化率增大,结焦率变化不大.结果表明,反应温度为430 ℃、初始氢气压力为7.0 MPa和反应时间为20 min是现有渣油加氢工艺的最佳反应条件.     

自源无线温度检测技术的研究与应用

基于温差模块、DS18B20和红外技术的自源无线温度检测技术,利用故障发热部位与环境的温度差发电自给,解决高压现场内难以获得低压电源的问题;利用红外线传输信号,避免在高压变电站中布线和信号受高压现场的强电磁干扰.实验结果表明,半导体温差模块输出电压与温差在一定范围内呈线性关系,温差越大,输出电压越大.采用带有超低压启动器的DC/DC电压提升电路进行电压提升,解决实际应用中由于温差过小而导致的半导体温差模块输出电压过低,不足以提供所需的电压问题.