Interferon-β Activity Is Affected by S100B Protein

Interferon-β (IFN-β) is a pleiotropic cytokine secreted in response to various pathological conditions and is clinically used for therapy of multiple sclerosis. Its application for treatment of cancer, infections and pulmonary diseases is limited by incomplete understanding of regulatory mechanisms of its functioning. Recently, we reported that IFN-β activity is affected by interactions with S100A1, S100A4, S100A6, and S100P proteins, which are members of the S100 protein family of multifunctional Ca²⁺-binding proteins possessing cytokine-like activities (Int J Mol Sci. 2020;21(24):9473). Here we show that IFN-β interacts with one more representative of the S100 protein family, the S100B protein, involved in numerous oncological and neurological diseases. The use of chemical crosslinking, intrinsic fluorescence, and surface plasmon resonance spectroscopy revealed IFN-β binding to Ca²⁺-loaded dimeric and monomeric forms of the S100B protein. Calcium depletion blocks the S100B–IFN-β interaction. S100B monomerization increases its affinity to IFN-β by 2.7 orders of magnitude (equilibrium dissociation constant of the complex reaches 47 pM). Crystal violet assay demonstrated that combined application of IFN-β and S100B (5–25 nM) eliminates their inhibitory effects on MCF-7 cell viability. Bioinformatics analysis showed that the direct modulation of IFN-β activity by the S100B protein described here could be relevant to progression of multiple oncological and neurological diseases.     

罗丹明B的飞秒双光子荧光显微成像研究

利用宽带飞秒振荡器作为激发光源搭建了双光子荧光显微成像系统,在飞秒脉冲的宽带激发下,测量了罗丹明B溶液的双光子荧光光谱,开展了罗丹明B固体样品的双光子荧光显微成像研究。在双光子荧光显微成像研究中,通过调节激发脉冲的功率,分析了双光子荧光强度和激发脉冲功率之间的关系,并且利用半波片改变线偏振激发光场的偏振方向,研究了双光子荧光强度随激发脉冲偏振方向的变化趋势,实现了双光子荧光强度的类正弦调制。     

MIS开发中c/s模式与b/s模式的比较分析

随着Internet的发展,管理信息系统(MIS)的发展迈进了新时代,企业MIS开发大量应用Web Server新技术,但不能将此新模式完全替代传统模式,或者与传统模式完全对立,应发挥各自长处,找到最佳结合方法,基于此,本文将两种开发模式加以比较.     

Human MicroRNAs Attenuate the Expression of Immediate Early Proteins and HCMV Replication during Lytic and Latent Infection in Connection with Enhancement of Phosphorylated RelA/p65 (Serine 536) That Binds to MIEP

Attenuating the expression of immediate early (IE) proteins is essential for controlling the lytic replication of human cytomegalovirus (HCMV). The human microRNAs (hsa-miRs), miR-200b-3p and miR-200c-3p, have been identified to bind the 3′-untranslated region (3′-UTR) of the mRNA encoding IE proteins. However, whether hsa-miRs can reduce IE72 expression and HCMV viral load or exhibit a crosstalk with the host cellular signaling machinery, most importantly the NF-κB cascade, has not been evaluated. In this study, argonaute-crosslinking and immunoprecipitation-seq revealed that miR-200b-3p and miR-200c-3p bind the 3′-UTR of UL123, which is a gene that encodes IE72. The binding of these miRNAs to the 3′-UTR of UL123 was verified in transfected cells stably expressing GFP. We used miR-200b-3p/miR-200c-3p mimics to counteract the downregulation of these miRNA after acute HCMV infection. This resulted in reduced IE72/IE86 expression and HCMV VL during lytic infection. We determined that IE72/IE86 alone can inhibit the phosphorylation of RelA/p65 at the Ser⁵³⁶ residue and that p-Ser⁵³⁶ RelA/p65 binds to the major IE promoter/enhancer (MIEP). The upregulation of miR-200b-3p and miR-200c-3p resulted in the phosphorylation of RelA/p65 at Ser⁵³⁶ through the downregulation of IE, and the binding of the resultant p-Ser⁵³⁶ RelA/p65 to MIEP resulted in a decreased production of pro-inflammatory cytokines. Overall, miR-200b-3p and miR-200c-3p—together with p-Ser⁵³⁶ RelA/p65—can prevent lytic HCMV replication during acute and latent infection     

基于ARM的三种无线家庭网关的设计方案及比较分析

用不同芯片PLC2214、S3C2410、S3C4510B设计的三种家庭网关方案,并对它们实现无线组网所采用的蓝牙、Zigbee、红外线技术进行了比较分析。     

基于嵌入式示波器的光子信号采集及自相关算法的设计

基于TDS5052B数字示波器设计了一种简化的光子信号采集和自相关算法。基于示波器中的嵌入式Windows2000操作系统,应用LabVIEW和VisualC++软件,实现了光子信号的高速采集以及基于多-tau原理的数字相关。与BrookHaven光子相关光谱仪进行比较,结果表明,该光子信号采集与自相关算法是可行的。     

镁合金表面超声滚压预处理对微弧氧化膜耐蚀性能的影响

镁合金基体首先进行超声滚压预处理后再进行微弧氧化镀膜,结合OM、SEM、EDS、XRD与电化学工作站(模拟体液PBS)对有无超声滚压处理的微弧氧化膜层性能进行测试分析,研究超声滚压处理对镁合金微弧氧化膜层性能的影响.结果 表明:超声滚压处理后镁基体表面粗糙度降低、晶粒细化且硬度提升;与镁合金直接微弧氧化的膜层相比,超声...     

Tailoring ZE21B Alloy with Nature-Inspired Extracellular Matrix Secreted by Micro-Patterned Smooth Muscle Cells and Endothelial Cells to Promote Surface Biocompatibility

Delayed surface endothelialization is a bottleneck that restricts the further application of cardiovascular stents. It has been reported that the nature-inspired extracellular matrix (ECM) secreted by the hyaluronic acid (HA) micro-patterned smooth muscle cells (SMC) and endothelial cells (EC) can significantly promote surface endothelialization. However, this ECM coating obtained by decellularized method (dECM) is difficult to obtain directly on the surface of degradable magnesium (Mg) alloy. In this study, the method of obtaining bionic dECM by micro-patterning SMC/EC was further improved, and the nature-inspired ECM was prepared onto the Mg-Zn-Y-Nd (ZE21B) alloy surface by self-assembly. The results showed that the ECM coating not only improved surface endothelialization of ZE21B alloy, but also presented better blood compatibility, anti-hyperplasia, and anti-inflammation functions. The innovation and significance of the study is to overcome the disadvantage of traditional dECM coating and further expand the application of dECM coating to the surface of degradable materials and materials with different shapes.     

Turbulent natural convection in an enclosure with localized heating from below

This study reports the results of a numerical investigation of turbulent natural convection in a square enclosure with localized heating from below and symmetrical cooling from the vertical side walls. The present study simulates the case of an accidental heat generation due to fire in a typical isolated building of a nuclear reactor or electronic components cabin. The source of fire is considered to be centrally located at the bottom wall with different heated widths, which is assumed to be either isothermal or with isoflux. For the purpose of the analysis, the source length is varied from 20 to 80% of the total width of the bottom wall. The top wall and the unheated portion of the bottom wall are considered to be adiabatic, whereas sidewalls are isothermal. Steady as well as transient forms of two-dimensional Reynolds–Averaged-Navier–Stokes equations and conservation equations of mass and energy, coupled with the Boussinesq approximation, are solved by the control volume based discretisation method employing the SIMPLE algorithm for pressure–velocity coupling. Turbulence is modeled using the standard k–ε model. Rayleigh number, Ra, based on the enclosure height is varied from 10⁸ to 10¹². Stream lines and isotherms are presented for various combinations of Ra and the heated width. A double cell flow pattern is observed with marginal loss in symmetry as Ra increases. The results are reported in the form of local and average Nusselt number on the heated floor. Correlations are developed to predict the heat transfer rates from the enclosure as a function of dimensionless heated width of the bottom wall and Ra, by least square linear regression analysis.     

DF5B型低恒速遥控机车的电气控制系统

介绍了DF5B型低恒速遥控机车的电气总体控制方式,包括遥控装置的组成和控制方式、DLC微机控制系统以及DLC与遥控装置信号转换和传送的控制电路。