Synthesis of surface imprinted polymers based on wrinkled flower-like magnetic graphene microspheres with favorable recognition ability for BSA

Nowadays,the employing of molecular imprinting technique in the analysis and separation of proteins from complex biological samples has been widely favored by researchers.To enrich the types of surface protein imprinted materials and expand the application fields of graphene materials,novel surface molecular imprinted polymers (MIPs) based on magnetic graphene microspheres Fe304@rGO@MIPs are first synthesized in this paper.Fe304@rGO@MIPs are prepared by oxidative self-polymerization of dopamine on the surface of magnetic graphene (Fe304@rGO) composite microspheres.Bovine serum albumin (BSA) is selected as protein template.Fe3O4@rGO microspheres with wrinkled flower-like structure are obtained by compounding Fe3O4 and graphene oxide in an appropriate ratio via the method of high-temperature reduction self-assembly.The microspheres exhibit promising dispersibility,high external surface area,rich pore structure,and sufficient magnetic properties.These advantages not only prevent the agglomeration of imprinted microspheres in the aqueous phase,which is conducive to contact and static adsorption,but also increase the amount of protein imprinting.Additionally,sufficient magnetic properties ensure fast and effective separation of the adsorbents.While the adsorption capacity is increased,the separation procedure becomes simple.The binding capacity of Fe304@rGO@MIPs for BSA can reach 317.58 mg/g within 60 min,and the imprinting factor (IF) is 4.24.More importantly,Fe3O4@rGO@MIPs can specifically recognize the target BSA from the mixed proteins and the actual sample.There is no significant decrease in the adsorption amount,IF,and magnetic properties after eight runs.It is promising to be used in the separation of proteins from the actual biological samples.     

Symbol Detection Based on Back Tracking Search Algorithm in MIMO-NOMA Systems

One of the most important methods used to cope with multipath fading effects, which cause the symbol to be received incorrectly in wireless communication systems, is the use of multiple transceiver antenna structures. By combining the multi-input multi-output (MIMO) antenna structure with non-orthogonal multiple access (NOMA), which is a new multiplexing method, the fading effects of the channels are not only reduced but also high data rate transmission is ensured. However, when the maximum likelihood (ML) algorithm that has high performance on coherent detection, is used as a symbol detector in MIMO NOMA systems, the computational complexity of the system increases due to higher-order constellations and antenna sizes. As a result, the implementation of this algorithm will be impractical. In this study, the backtracking search algorithm (BSA) is proposed to reduce the computational complexity of the symbol detection and have a good bit error performance for MIMO-NOMA systems. To emphasize the efficiency of the proposed algorithm, simulations have been made for the system with various antenna sizes. As can be seen from the obtained results, a considerable reduction in complexity has occurred using BSA compared to the ML algorithm, also the bit error performance of the system is increased compared to other algorithms.     

A label-free amperometric immunosensor based on horseradish peroxidase functionalized carbon nanotubes and bilayer gold nanoparticles

In this work, a novel label-free amperometric immunosensor has been constructed for detecting α-1-fetoprotein (AFP) based on nanocomposite of horseradish peroxidase (HRP) labeled carbon nanotubes (CNTs). First, the gold nanoparticles (AuNPs) were electrodeposited on the surface of the glass carbon electrode by electrochemical reduction of gold chloride tetrahydrate (HAuCl₄) to immobilize horseradish peroxidase labeled carbon nanotubes (HRP-CNTs). Then HRP-CNTs bioconjugate was immobilized on the surface of the electrodeposited AuNPs layer by the combination of forces (coordination and electrostatic force). Subsequently, it was immersed into gold colloidal nanoparticles (GNPs) solution, which was used to immobilize antibody biomolecules (anti-AFP). Enhanced sensitivity was obtained by using bioconjugates featuring HRP labeled (HRP-CNTs), which had lager specific surface area and good electronic catalysis (current response signal) compared to carbon nanotubes. Under optimized conditions, the linear ranges were from 0.2 to 200 ng mL⁻¹ with a detection limit of 0.067 ng mL⁻¹ (at an S/N of 3). The proposed immunosenor showed good precision, acceptable stability and reproducibility and could be used for the detection AFP in normal human serum, which provided a potential alternative tool for the detection of protein in clinical diagnosis.     

Distributed and resilient localization algorithm for Swarm Robotic Systems

Many applications of Swarm Robotic Systems (SRSs) require each robot to be able to discover its own position. To provide such capability, some localization methods have been proposed, in which the positions of the robots are estimated based on a set of reference nodes in the swarm. In this paper, a distributed and resilient localization algorithm is proposed based on the BSA–MMA algorithm, which uses the Backtracking Search Algorithm (BSA) and the Min–Max Area (MMA) confidence factor. It is designed in a novel four-stage approach, where a new method, called Multi-hop Collaborative Min–Max Localization (MCMM), is included to improve the resilience in case of failures during the recognition of the reference nodes. The results, obtained with real Kilobot robots, show 28–36% of performance improvement obtained by the MCMM. Also, it is shown that the final result of the localization process is better when the MCMM is executed than if it is not executed. The experiments outcomes demonstrate that the novel four-stage approach and the use of the MCMM algorithm represents a progress in the design of distributed localization algorithms for SRS, especially with regard to its resilience.     

Antioxidant and antiglycation properties of Passiflora alata and Passiflora edulis extracts

The leaves of Passiflora alata Dryander and Passiflora edulis Sims, traditionally used in American countries to treat both anxiety and nervousness by folk medicine, are rich in polyphenols, which have been reported as natural antioxidants. In this study, the antioxidant activities of P. edulis and P. alata hydroalcoholic leaf extracts were verified in in vitro and ex vivo assays. P. alata showed a higher total reactive antioxidant potential than did P. edulis. The antioxidant activities of both extracts were significantly correlated with polyphenol contents. In addition, both extracts attenuated ex vivo iron-induced cell death, quantified by lactate dehydrogenase leakage, and effectively protected against protein damage induced by iron and glucose. These findings demonstrate that the P. alata and P. edulis leaf extracts have potent in vitro and ex vivo antioxidant properties and might be considered as possible new sources of natural antioxidants.     

一种实用的毫米波大规模MIMO混合预编码算法

数字模拟混合预编码可以用较少的射频逼近全数字预编码的性能,可以用来解决毫米波大规模MIMO系统中由于射频链路过多造成的硬件损耗和校准问题。为解决传统混合预编码结构难以实现的缺点,该文的混合预编码研究基于一种简单的固定子连接结构。推导了系统可达速率最大,模拟预编码矩阵应满足的条件,从而将混合预编码矩阵设计问题转化为优化问题。采用鸟群算法(BSA)解决此优化问题,求得最优的预编码矩阵。针对模拟移相器分辨率有限的情况,提出一种直接量化的解决方案和一种基于改进的离散BSA的解决方案。仿真结果表明,所提算法能够基于简单结构实现较好的性能;移相器分辨率有限情况下,所提的两种解决方案都是有效的,且基于离散BSA的方案在分辨率较低时性能更优。     

不同气氛低气压电容耦合放电等离子体对聚甲基丙烯酸甲酯表面的改性

利用低气压电容耦合放电等离子体对聚甲基丙烯酸甲酯(PMMA)表面进行亲水改性,对比分析了Ar、N₂、Air和O₂四种等离子体放电气体和不同放电功率对其表面的影响。采用静态接触角、原子力显微镜、X射线光电子能谱分别对PMMA表面亲水性、粗糙程度以及元素和官能团的组成进行了表征。以牛血清蛋白作为标准蛋白,检测处理后PMMA表面蛋白吸附量。结果表明,经等离子体处理后的PMMA表面亲水性和抗蛋白性能均有不同程度的改善,其中Ar等离子体主要起刻蚀的作用,N₂、Air和O₂等离子体在对PMMA刻蚀的同时,接枝的官能团对其表面性能的改变起到主导作用。当等离子体放电功率较低时,增加功率可以显著提高表面亲水性,且亲水性能的提高带动抗蛋白性能的增强。     

分子对接和荧光光谱法研究麦角甾醇与牛血清白蛋白的相互作用

用分子荧光光谱实验法和分子对接理论研究麦角甾醇与牛血清白蛋白（bovine serum albumin,BSA）的相互作用。荧光光谱实验结果表明：麦角甾醇能猝灭BSA的内源性荧光,其猝灭类型为静态猝灭;通过考察猝灭过程中热力学函数的变化初步推断麦角甾醇与BSA的结合是自发的熵增过程,驱动力主要为疏水相互作用。运用分子对接技术研究了麦角甾醇与BSA的相互作用,结果表明：麦角甾醇与BSA相结合,主要的作用力类型为疏水相互作用;并获得了麦角甾醇在BSA中的作用位点,麦角甾醇处在一个疏水性的结合口袋中,结合稳定性强。荧光光谱的实验结果与分子对接的理论结果总体上一致,说明结合过程是一个自发的过程,BSA可以携带和运输麦角甾醇,同时从分子对接中获得了麦角甾醇在BSA中详细的结合位点和结合模式。     

氯霉素全抗原的合成与鉴定

目的：为获得较高偶联率氯霉素全抗原,探索和优化碳二亚胺法(EDC)合成氯霉素(CAP)与牛血清白蛋白(BSA)全抗原合成条件。方法：采用单因素设计,优化影响合成的主要因素：蛋白浓度、CAP 与BSA 的摩尔比例、反应体系pH 值,通过紫外法和三硝基苯磺酸(TNBS)法测定合成后的偶联率。 结果：EDC 法合成氯霉素抗原较优条件为BSA 浓度15mg/ml、CAP:BSA=70:1、pH7.4,可得到偶联率为15 左右的氯霉素全抗原。结论：EDC法可用于制备较高偶联率的氯霉素全抗原,以用来进行抗体制备。     

The methionine precursor dl-2-hydroxy-(4-methylthio)butanoic acid protects intestinal epithelial barrier function

dl-2-hydroxy-(4-methylthio)butanoic acid (HMTBA) is a source of dietary methionine (Met) that is widely used in poultry nutrition. We have previously shown that HMTBA is preferentially diverted to the transsulfuration pathway, which gives antioxidant metabolites such as taurine and glutathione. Therefore, here we hypothesize that this Met source can protect epithelial barrier function in an in vitro model of intestinal inflammation of Caco-2 cells. The results show that HMTBA prevents the increase in paracellular permeability induced by H₂O₂ or tumour necrosis factor-α. This effect can be attributed to the increased production of taurine and reduced glutathione. Similar results were obtained for dl-Met, although the protective role of the amino acid was less pronounced than that of the hydroxy analogue. In conclusion, the diversion to the transsulfuration pathway means that this Met precursor is of greater value than previously thought, due to its capacity to improve intestinal homeostasis and the quality of poultry products destined for human consumption.