DNA计算求解连续空间优化问题

首先构造了二进制编码的网络空间,其两个节点连接权的四条边对应到DNA编码,设计了相应的算子起到DNA计算酶的作用,该算法设计使得DNA计算能够求解连续空间优化问题。通过测试函数表明该算法具有较好的收敛速度和稳定性,求解结果非常好。     

鱼精DNA的快速无污染提取工艺

鱼类精是一秀作为废弃物处理，鱼精中含有大量的脱氧核糖核酸（ＤＮＡ），ＤＮＡ在医药上具有多种用途。本文研究从鱼精中提取ＤＮＡ的工艺过程，提取率为１５－２５％，成品ＤＮＡ纯度为９６．８５％，本工艺简单、快速、无污染，适合于工业化生产ＤＮＡ《     

DETERMINATION OF BREWING YEAST PLOIDY BY DNA MEASUREMENT

A method has been derived which permits the determination of the degree of ploidy in industrial strains of yeast. The method involves measuring the DNA content per cell of the strains, which is achieved by careful counting of the cells using a haemocytometer and a determination of the amount of DNA by a colorimetric method. Using this technique it was determined that the two industrial lager strains under investigation are tetraploid. * 1 These results have been briefly described at the meeting of the EBC Subgroup on Yeast Genetics, Copenhagen 1982.
     

MULTI-WALL CARBON NANOTUBE-BASED DNA NANOSENSOR FOR DETERMINING MITOXANTRONE-DNA INTERACTION IN-VITRO

The present study involves the development of a carbon nanotube based DNA nanosensor to determine the toxicological behavior of mitoxantrone (MTX). Mitoxantrone intercalates with DNA and produces a MTX-DNA adduct, resulting in the blockade of protein synthesis and excessive production of free radicals in the myocardium which eventually leads to cardiac toxicity. So, our work employs a DNA nanosensor to investigate the interaction of MTX with DNA. Multi-wall carbon nanotubes (MWCNTs) were functionalized with carboxyl group and were used for immobilization of DNA to construct the DNA nanosensor. The DNA nanosensor was immersed in MTX solution to monitor MTX-DNA interaction with respect to time and alter the resistance of the nanosensor. It was observed that MTX-DNA interaction is fast initially and as time elapses, the change in interaction gets slow due to formation of MTX-DNA adduct. The limit of detection (LOD) and limit of quantification (LOQ) were found as 150 (ng/mL) and 456 (ng/mL), respectively, for DNA nanosensor. This study suggests that the potentiometric nanosensor allows real-time monitoring of the drug-DNA interaction changes by measuring the potential at DNA/sensor interface which can prove to be an important tool in drug discovery pipelines and molecular toxicology.     

杂交型DNA电化学生物传感器研究进展

杂交型DNA电化学生物传感器是一类利用核酸互补配对杂交原理检测和分析特定DNA序列的电化学生物传感器。由于其具有快速简便、选择性好、灵敏度高等优点,在临床医学、遗传工程、环境检测、食品安全监测和生物科学等领域有着重要的应用价值。简述了杂交型DNA电化学生物传感器的一般原理,对共价键结合法、自组装法、生物素-亲和索法、电聚合法以及吸附法等单链DNA的固定方法和DNA杂交信号的直接和间接电化学转换机制的近期研究进展进行了深入探讨,并对其在医疗检测和转基因植物检测等基因检测方面的最新应用和发展趋势进行了论述。     

Matrix splicing system

The string splicing system was introduced by Tom Head, which stands as an abstract model for the DNA recombination under the influence of restriction enzymes and ligase enzymes. However, it is observed that strings are not very capable of modelling the complex chemical process. Hence, in this article, matrix splicing system is introduced with a new splicing operation among the matrices. It is shown with an illustration that the DNA recombination process can be simulated by the matrix splicing system, which justifies the suitability of the matrices for modelling the complex processes of the molecules. The properties of the components of the system such as cutting rules and the splicing rules are discussed. Besides some properties of the system, it is shown that this system stands as a theoretical model for splicing strings in parallel. The abstraction of the ligase enzymes is explained which is missing in all the splicing models that have come out so far. The ability of the system in generating new patterns is also outlined.     

配合物[Cd（phen）2O2]的结构及其与脱氧核糖核酸（DNA）的相互作用

合成一种新的镉配合物[Cd（phen）2O2]（其中phen：1,10-菲哆啉）,并用X射线单晶衍射仪确定配合物的晶体结构,用荧光光谱研究该配合物与脱氧核糖核酸HC-DNA（Hela细胞DNA）的作用规律,用凝胶电泳法研究该配合物对pBR322-DNA的切割作用,从插入方式角度讨论配合物与DNA相互作用机理。     

新型基因枪微弹材料可行性初步研究-Ⅰ

基因枪在未来临床基因转导中很有前途,但目前所用微弹材料金、钨都属于重金属,其长期存在于人体的安全性尚无法估计。提出用羟基磷灰石(HAP)微粒来代替金或钨微粒,从理论上推导了这种取代的可行性,并报告了一种HAP微粒的制备及其负载DNA的研究结果。理论推导完全按照物理学方法进行。实验部分的方法:将稳定剂H以一定速度和比例滴入一定浓度的Ca(H2PO4)2溶液中,再按照一定的速度和比例滴入Ca(OH)2饱和溶液,期间或完成后超声处理一定时间,将所得HAP微粒溶液与亚精胺和DNA以一定比例和浓度在一定pH条件下混合。结论:所得HAP粒径值符合要求,并且能够高效负载DNA。     

结核分枝杆菌融合抗原Ag85B-ESAT6真核表达载体的构建及鉴定

以结核分枝杆菌H37Rv株的基因组作为模板,将Ag85B和ESAT6编码基因进行PCR扩增,然后采用基因剪接重叠扩增PCR法(gene SOEing)将其通过疏水甘氨酸接头(GGIGIAPG)连接融合,定向克隆至质粒Pvax1中,构建结核分枝杆菌Ag85B-ESAT6融合抗原的真核表达质粒Pvax1/AE.经单双限制性内切酶图谱、PCR产物及DNA测序分析等多种方法鉴定,证实Pvax1/AE真核表达质粒构建成功.为进一步研究其结核核酸疫苗原型的免疫保护效果奠定了基础.     

压电基因传感器检测芽孢杆菌靶序列研究

在石英晶体微天平(QCM)上用生物素亲和素法和自组装法2种不同的方法固定寡核苷酸探针,构建压电基因传感器,对芽孢杆菌靶序列进行实时检测。结果表明:生物素亲和素法固定探针效果更好,靶序列浓度为0.05~0.5μmol/L时,有很好的线性关系,线性回归方程为Y=93.88X+10.88(R=0.989 0,N=6,P<0.001),非线性误差为±4.7%。该传感器特异性较好,能够识别错配3个碱基的序列。