pH-sensitive capsules as intracellular optical reporters for monitoring lysosomal pH changes upon stimulation

The concept of a long-term sensor for ion changes in the lysosome is presented. The sensor is made by layer-by-layer assembly of oppositely charged polyelectrolytes around ion-sensitive fluorophores, in this case for protons. The sensor is spontaneously incorporated by cells and resides over days in the lysosome. Intracellular changes of the concentration of protons upon cellular stimulation with pH-active agents are monitored by read-out of the sensor fluorescence at real time. With help of this sensor concept it is demonstrated that the different agents used (Monensin, Chloroquine, Bafilomycin A1, Amiloride) possessed different kinetics and mechanisms of action in affecting the intracellular pH values.     

Advanced Subcompartmentalized Microreactors: Polymer Hydrogel Carriers Encapsulating Polymer Capsules and Liposomes

The design of compartmentalized carriers for advanced drug delivery systems or artificial cells and organelles is of interest for biomedical applications. Herein, a polymer carrier microreactor that contains two different classes of subcompartments, multilayered polymer capsules and liposomes, is presented. 50 nm‐diameter liposomes and 300 nm‐diameter polymer capsules are encapsulated into a larger polymer carrier capsule, demonstrating control over the spatial positioning of the subcompartments, which are either ‘membrane‐associated’ or 'free‐floating’ in the aqueous interior. Selective and spatially dependent degradation of the 300 nm‐diameter subcompartments (without destroying the structural integrity of the enzyme‐loaded liposomes) is also shown, by performing an encapsulated enzymatic reaction using the liposomal subcompartments. These findings cover several important aspects toward the development of engineered compartmentalized carrier vessels for the creation of artificial cell mimics or advanced therapeutic delivery systems.     

高湿环境对洗衣凝珠安全性能的影响

在高湿环境条件下,洗衣凝珠吸水增重后,其耐压强度随着水分含量的增加而衰减,当吸水量达到8％左右(水分含量约16％)时,耐压强度会下降50％～70％.随着洗衣凝珠市场份额不断增加,洗衣凝珠逐渐普及,仅百度搜索"误食洗衣凝珠"就有三千多万相关结果,我们需关注高湿条件下对其安全性能挑战,建议生产厂家充分考虑洗衣凝珠耐压强度指...     

岭南水乡顺德逢简村水环境保护及发展研究

本文以顺德水乡中具备典型岭南水乡特征的逢简水乡为例证,通过对水环境的功能、特征分析研究水环境保护及发展的对策,做好河涌绿道的生态建设,恢复水乡文化和景观。     

紫外-可见分光光度法测定盐酸氟桂利嗪胶囊的含量

为了建立盐酸氟桂利嗪生产过程中的快速分析方法,采用紫外-分光光度法和高效液相色谱法对盐酸氟桂利嗪进行测定。对比结果表明,以盐酸为溶剂,选择253 nm为测定波长,在盐酸氟桂利嗪为7.488～17.472μg.mL-1的浓度范围内,紫外-分光光度法与高效液相色谱法具有很好的可靠性,可以作为生产过程质量控制的快速测定方法。     

气相色谱法测定痛泻软胶囊中柠檬烯的含量

目的:建立用气相色谱外标法分析痛泻软胶囊中柠檬烯的方法。方法:采用SE-30色谱柱,50~240℃程序升温,火焰离子化检测器检测。结果:柠檬烯能与其它组分很好的分离,分离度(α)>2.0,且峰型对称。柠檬烯浓度在0.002014～2.014 mg/mL范围内,r=0.9995,呈现良好的线性关系,检出限为0.0008%。采用的样品处理方法回收率高且对色谱柱及检测器的污染小。结论:该法简便、快速、准确性高、重复性好,可作为本品的质量控制方法。     

Study on Microbial Communties during the Fermentation Process of Feng ＆ Composite-flavor Taibai Liquor（II）：Microbial Communities in Fermented grains

对凤兼复合型太白酒发酵过程中糟醅微生物区系的多样性、构成及演替规律进行研究,并通过形态、生理学特性的分析比较和16S rRNA基因序列分析,对发酵过程中主要微生物类群进行了分类鉴定。     

新型植物基载体对乳糖酶的高效递送及保护

为解决口服酶制剂易被胃液的强酸环境破坏其效价,影响乳糖不耐受症治疗效果的问题,制备一种可以保护乳糖酶过胃而不失去活性的新型植物基载体。采用新型植物基材料向日葵孢粉素外壁胶囊为载体,用于乳糖酶的负载及保护。通过负载、包膜、释放等实验以及采用扫描电子显微镜、激光共聚焦显微镜等表征手段,确定5%海藻酸钠与6%羧甲基茯苓多糖在体积比为1∶1混合所得的凝胶球体外释放效果最佳,实现在消化道中的响应性释放。进一步实验发现,经冻干处理后该体系也具备成为乳糖酶冻干保护剂的潜力。基于此,实验成功论证了向日葵孢粉素外壁胶囊递送体系可以作为乳糖酶的高效递送及保护载体的可行性,为乳糖不耐受症的治疗提供了新的思路。     

鱼油软胶囊中脂肪酸组成及比率分析

采用气相色谱法对海洋鱼油软胶囊中脂肪酸组成和比率进行分析,为合理补充DHA、EPA等多不饱和脂肪酸提供理论依据,并为鉴别海洋鱼油质量提供基础数据。结果表明:鱼油软胶囊中主要含有C14~C22脂肪酸,其中不饱和脂肪酸质量分数50.8%~85.1%,特别是具有重要生理作用的多不饱和脂肪酸,如C20:5(EPA,约占10.0%~35.1%)、C22:6(DHA,约占10.7%~20.5%);鱼油软胶囊中n-3不饱和脂肪酸质量分数约为22.5%~55.7%,n-6不饱和脂肪酸与n-3不饱和脂肪酸的比率约为0~0.7,EPA与DHA的比率约为0.8~1.9。