抗大肠杆菌荧光抗体的制备及其特性

活体大肠杆菌与弗氏不完全佐剂乳化后注射到新西兰大白兔体内15d后,利用两步沉淀法提取并纯化得到大肠杆菌表面蛋白抗体IgG,与FITC的交联率F/P达到2.7,未发现FITC对大肠杆菌表面蛋白抗体的特异性有显著的影响。将抗大肠杆菌荧光抗体注入小白鼠体内后,除肠道内有荧光显示外,肝脏、心脏、肾脏中未发现荧光显示,表明大肠杆菌只在小白鼠大肠存在,根据观测到的荧光强度可定性和定量地判断大肠中的大肠杆菌。     

一种480MHz无线数传模块的设计

针对中国智能电网开放的免费无线计量频段,设计了一种可工作在470 MHz～510 MHz、中心频率为480 MHz、输出功率可调的无线数传模块。模块采用MSP430F2274单片机和射频芯片CC1100E设计,利用EDA软件ADS2008仿真优化了射频电路的输出匹配网络。最后对无线模块输出功率、通信距离等参数进行了测试和验证。     

4,4’-二氨基二苯砜固化环氧树脂/蒙脱土纳米复合材料反应动力学的研究

选择4,4’-二氨基二苯砜（DDS）作为固化剂,采用非等温DSC法研究了双酚A缩水甘油醚型环氧树脂（E-51）/有机蒙脱土（MMT）纳米复合材料的固化反应动力学,根据Kissinger方程计算出该反应的表观活化能（ΔE）为59.91 kJ/mol,基于Crane方程计算得到该反应的反应级数为0.89,表明该反应为一复杂反应,根据固化反应动力学测试结果确定了E-51/DDS/MMT体系固化成型工艺.     

如何当好电子政务系统中的甲方代表

本文借助建筑工程项目中甲方代表的定义、职责和权利范围等内容,依据软件开发各阶段需要完成的任务,讨论软件项目开发中甲方代表该履行的职责。随后,分析电子政务系统中,甲方代表将面临的窘境,说明他们工作的难点,并根据电子政务系统建设的特点,提出电子政务项目中当好甲方代表的若干建议,也指出如何培养甲方代表,让他们在工作中实际担负哪些责任,是需要进一步研究的问题。     

河南省能源-经济-环境系统协调发展实证研究

建立了一个能源-经济-环境系统综合发展评价指标体系,并运用主成分聚类分析方法对河南省18个地市的能源、经济、环境（3E）系统进行了研究．结果表明：河南省各地市3E系统存在着较大的不协调,可分为规模主导型、资源匮乏型和资源主导型3类．最后提出了促进能源、经济、环境系统协调发展的对策和建议．     

工业以太网在五矿营口中板厂1、2号高炉新建项目中的应用

魏德米勒管理型工业以太网交换机IES21系列专为工业恶劣环境所设计,具有可靠的冗余功能,成为工业控制网络中通信设备的理想选择。在五矿营口中板厂1、2号高炉新建项目高炉控制子系统中,PLC设备连在环形以太网上,既相互独立又数据共享,实现了整个高炉系统的数据采集、逻辑控制及电气传动自动化。     

The "hidden observer" phenomenon in hypnosis: Some additional findings.

23 highly hypnotizable undergraduates (Harvard Group Scale of Hypnotic Susceptibility) underwent 2 specially constructed 7-item hypnotic inductions. Over the 14 items, the main finding was of a relationship between E. R. Hilgard's (1973, 1977, 1979) "hidden observer" effect and 2 aspects of hypnotic age regression. Ss reporting a hidden observer experienced duality during regression to age 5, in which they were aware of being both adult and child. When asked to write a complex sentence, most did so, usually without spelling errors. The Ss not reporting this effect experienced quasi-lateral age regression in which they had the exclusive feeling of being 5 yrs old, with no sense of an adult identity. Most of these Ss were unable to write the same complex sentence when requested to during age regression. The study replicated Hilgard's finding of the hidden observer phenomenon in terms of its incidence and obtained similar verbal reports from Ss experiencing it. At the same time, the results suggest that a neodissociation account of hypnosis may need some modifications to accommodate these additional findings. (27 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

在大肠杆菌K99(中国QH株)菌毛形成亚单位基因(fanC)的克隆和测序中发现IS1 插入序列

目的 构建大肠杆菌K99菌毛表达载体。方法 通过逐级亚克隆的方法克隆k99菌毛形成亚单位基因（fanC），并进行序列测定和fanC亚单位的抗原决定簇模拟，以确定突变部位。结果 克隆了包括K99菌毛形成亚单位基因（fanC）和部分fanD基因在内的一段基因，该基因与国外报道相比明显偏大。序列测定表明：在fanC尾部多出 24bp，在fanC与 fanD，的结合部有一 800bp左右的新基因。经与基因库中大肠杆菌基因作同源性比较发现，该新基因为768bp的插入序列IS1。其他基因为K99自身fonC与fanD片段之间的过渡基因。IS1的插入未改变fanD，基因的阅读框架，只是使fanD片段末端增加了 8个氨基酸。fanC亚单位的抗原决定簇模拟结果显示，K99 fanC片段具有一个非常突出的亲水区域，根据经验它应是抗原决定簇所在的部位。结论 首次发现大肠杆菌K99基因中含有插入序列IS1。     

C8051F020单片机的特点及其在通信系统中的应用

文中介绍了C8051F020单片机的功能特点,并给出了C8051F020单片机应用于通信系统中的方案。     

Mechanical properties of textile-inserted PP/PP knitted composites using injection–compression molding

This paper concentrates on the experimental investigation of the self-reinforced all-polypropylene composites. There exists an optimum processing condition to produce high quality specimens by injection–compression molding. Tensile and 3-point bending properties of the virgin PP materials were nearly unaffected by the introduction of reinforcing knit layer(s) due to very low fibre content of the knitted fabrics used. 3-point bending properties were also unaffected by the surface of indentation-flexure. The applied impact energy was maintained at 5 J for the homo-PP and 27 J for the block-PP materials, respectively, to cause penetration during drop-weight impact tests. It is interestingly noteworthy that the self-reinforced homo-PP composites exhibited superior energy absorption capability when compared with the virgin matrix materials. The corresponding plate bending performances of the self-reinforced homo-PP composites also revealed consistent improvement as compared to their virgin counterparts. On the other hand, although virgin block-PP material exhibited better impact performances than its composite reinforced by the homo-PP knitted fabric, a notably small increase in the reinforcement fibre content revealed considerable improvement in the impact properties comparable to those of the virgin block-PP matrix materials. These self-reinforced homo-PP/block-PP materials have clearly indicated that they have the potential to out-perform the block-PP materials via modification and/or manipulation of the reinforcement knit structural/geometrical parameters and the content of reinforcement fibres. Both static and dynamic impact properties are likely to be affected by the local area properties of the tested face under indentation, and thereby contributing to the improved performances of the composite specimens with the knit face under the impact.