GPRS数据分包长度和心跳包间隔性能分析

分析了车载GPRS数据传输的难点,在南京地区建立GPRS网络测试系统,测试结果表明:为确保高速率移动数据传输的可靠性和高效性,在GPRS网络中使用TCP协议时,心跳包间隔最大9 min;使用UDP协议时,心跳包间隔最大4min;数据分包长度小于500byte.该试验为实际工程应用中的参数设置提供了依据.     

浓香型白酒酿造环节中产酯化酶的霉菌多样性

对分离自宜宾多粮浓香型白酒酿造环节中的135株霉菌产酯化酶的能力进行了检测,发现40株菌具备产生酯化酶的能力。其中以来自窖房的最多(34株),分离自糟醅次之(4株),其余为曲药(1株)、曲房(1株)。通过对这40株霉菌开展的基于ITS nu-rDNA序列的系统发育分析,发现其分属于Penicillium、Aspergillus、Emericella、Rhizopus、Cladosporium、Mucor等6个属,以Penicillium(19株)和Aspergillus(13株)为主要类群。结果显示,宜宾多粮浓香型白酒酿造环节中具备大量产酯化酶能力的霉菌,而且这些霉菌存在较为丰富的系统发育多样性。     

贮藏“海沃德”猕猴桃中霉菌的分子鉴定及生物学特性的研究

目的:分离与鉴定陕西省周至县贮藏期腐烂\     

木醋杆菌ITS序列分析及产纤维素条件的研究

木醋杆菌(Acetobacter xylinum)是合成纤维素能力最强的细菌,设计木醋杆菌16S-23S rDNA转录间隔区(internally transcribed spacer ITS)序列引物,对其扩增.在GenBank上的登陆号为GenBank EU196159.选择到合适的木醋杆菌ITS扩增引物,PCR扩增条件.木醋杆菌最佳培养温度28℃,通过测定纤维素产量发现最佳接种量5%,最佳碳源为葡萄糖,最佳碳源浓度为2.5%.     

Molecular Identification of Dendrobium Species (Orchidaceae) Based on the DNA Barcode ITS2 Region and Its Application for Phylogenetic Study

The over-collection and habitat destruction of natural Dendrobium populations for their commercial medicinal value has led to these plants being under severe threat of extinction. In addition, many Dendrobium plants are similarly shaped and easily confused during the absence of flowering stages. In the present study, we examined the application of the ITS2 region in barcoding and phylogenetic analyses of Dendrobium species (Orchidaceae). For barcoding, ITS2 regions of 43 samples in Dendrobium were amplified. In combination with sequences from GenBank, the sequences were aligned using Clustal W and genetic distances were computed using MEGA V5.1. The success rate of PCR amplification and sequencing was 100%. There was a significant divergence between the inter- and intra-specific genetic distances of ITS2 regions, while the presence of a barcoding gap was obvious. Based on the BLAST1, nearest distance and TaxonGAP methods, our results showed that the ITS2 regions could successfully identify the species of most Dendrobium samples examined; Second, we used ITS2 as a DNA marker to infer phylogenetic relationships of 64 Dendrobium species. The results showed that cluster analysis using the ITS2 region mainly supported the relationship between the species of Dendrobium established by traditional morphological methods and many previous molecular analyses. To sum up, the ITS2 region can not only be used as an efficient barcode to identify Dendrobium species, but also has the potential to contribute to the phylogenetic analysis of the genus Dendrobium.     

露天矿智能运输系统的研究

介绍了大型露天矿智能运输系统的原理、组成与主要功能。     

A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily

Aspergillus strains belonging to section Nigri isolated during a two year survey in eight Sicilian vineyards located on the slopes of Mount Etna (Sicily, Italy) were analysed analyzed in order to characterize species responsible for ochratoxin A (OTA) contamination of grapes. The polyphasic approach permitted analysis of biodiversity of Aspergillus isolates in relation to their morphology, ochratoxigenicity and genetic variability. We assessed OTA production by A. carbonarius, A. niger, A. tubingensis and A. japonicus using an enzyme-linked immunosorbent assay. A. carbonarius isolates were the strongest OTA producers. A subset of 66 representative strains was selected for further DNA-based characterization. PCR assays using species-specific primers discriminated between A. niger, A. carbonarius and A. japonicus on the basis of the target sequences for each species. The PCR-based methods matched morphological characterization in identifying all the black aspergilli (BA) isolates tested, whereas RFLP analysis with RsaI of isolates positive to PCRs with A. niger specific primers identified three A. tubingensis isolates. The identification of thirteen isolates was further confirmed by ITS analysis. By this method, each of the isolates was identified and assigned to an Aspergillus species. The fAFLP analysis of 40 isolates highlighted the power of this technique to discriminate different species and single strains, to verify the presence of mixed populations in the same vineyard, through homogeneous species clusters. No correlation was observed between the clusters and OTA production level or origin.     

浅谈压力管道的检验

针对压力管道检验环节薄弱的现状,分析了各种检验手段,介绍了压力管道检验要点     

ITS中学生模型和教学策略的设计

本文介绍了ITS（Intelligent Tutoring System,智能导师系统）的工作模式、系统构成。建立了一种学生模型。根据此模型系统可以自动建立相应的教学策略，实现教学智能化。     

中文TTS系统中代价权值确定的方法

近年来,中文文语转换系统趋向于采用基于语料库上下文的单元选择法来获取拼接的语音单元实例,事实证明这种方法对于合成高质量的语音非常有效。单元选择的关键是各单元特征权值的确定,这里将提出一种非常有效的在单元选择中确定各特征权值的方法。它是对现有的利用线性回归确定权值方法的改进。其主要思路是对单元进行分类,对于各类单元分别进行线性回归,同时定义了一个客观衡量语音差异的距离,从而可以回归得到各特征的恰当权值。