Development of a Novel PCR Primer to Differentiate and Identify Bacillus subtilis and Closely Related Species Isolated from Thai Fermented Foods

A novel PCR assay based on 16S-23S internal transcribed spacers (ITS) length polymorphism was developed for rapid differentiation and identification of the Bacillus subtilis group, especially B. subtilis, B. licheniformis and B. amyloliquefaciens, the most frequently isolated bacilli from fermented foods. A new group-specific conserved primer pair, CITS-F and CITS-R, was designed for specific amplification of the ITS region in B. subtilis and closely related species. The fingerprints of seven reference species, B. subtilis, B. amyloliquefaciens, B. licheniformis, B. pumilus, B. atrophaeus, B. vallismortis and B. mojavensis using CITS-F and CITS-R primers showed the same four signature bands of 227, 400, 542 and 650 bp. They are different from those of other genera and species tested. Therefore, these four signature bands could be used to differentiate and identify the B. subtilis group. Moreover, the sequence of the 227 bp signature band could also be used to distinguish closely related species of the B. subtilis group used in this study. A novel PCR assay based on ITS length polymorphism pattern using CITS-F and CITS-R could be considered as a rapid and easy method for the primary differentiation of the B. subtilis group.     

基于ITS序列分析对疑似白羊肚菌株的分子鉴定

通过提取疑似白羊肚菌株基因组DNA,采用通用引物ITS1和ITS4扩增ITS序列,利用GenBank中的BLAST软件搜索同源序列进行比对,并构建系统发育树,对疑似菌株白羊肚进行了初步分子鉴定。结果表明,在GenBank数据库中登录的有26 个菌株与白羊肚ITS 序列相似度为99%,其中Pleurotus eryngii (杏鲍菇)15 个,P. nebrodensis(白灵菇)11 个。根据rDNA ITS 区序列分析准则和白羊肚菌褶呈网格状,菌盖为白色的形态特征,确定疑似白羊肚菌株为与白灵菇(P. nebrodensis)和杏鲍菇(P. eryngii)高度近缘的一个菌株。     

The Performance of Fiber-Radio Road Vehicle Communication System with Macro-Diversity

This paper proposes a road-to-vehicle communication system based onfiber-radio extension link (FREx link). Since the proposedroad-to-vehicle communication system interconnects a cell controlstation with base stations by FREx link, optical fiber link noiseshould be taken into account to evaluate the system. This paperanalyzes the outage probability and frequency utilization efficiencyperformance of the fiber-radio road-to-vehicle communication systemwith macro-diversity. It also shows that the proposed system withmacro-diversity can improve performance, although it should bedesigned with the effect of optical fiber link noise in mind.     

发展智能公交系统,提高武汉公交效率

对国内智能交通系统 (ITS)的需求及制约条件进行了分析 ,针对武汉市的具体交通状况 ,指出武汉市发展智能公交系统 (ITS)的当务之急是建设好公共交通系统 (APTS) ,并提出了武汉市APTS系统建设的方案与实施方式及具体步骤 .     

地图匹配技术及其在智能交通系统中的应用

地图匹配(Map Matching)技术利用电子地图和定位信息来确定车辆在道路上的准确位置,是一种软件纠错技术。该文在引入地图匹配问题的基础上,分析讨论了目前主要的地图匹配算法,并结合智能交通系统(ITS)的需求,探讨了车辆所在道路上具体位置的识别方法,最后对地图匹配技术在ITS中的应用进行了回顾和展望。     

黑龙江、吉林地区松茸ITS序列遗传多样性分析

提取黑龙江省5个不同产地和吉林省汪清地区不同产地松茸子实体的基因组DNA,利用ITS序列分析技术研究其遗传多样性。测序后通过和GenBank中序列比对,采用最大简约法(Maximum Parsimony,MP)、最小进化法(Minimum Evolution,ML)和邻位相接法(Neighbor Joining,NJ)构建系统发育树。结果表明,6个样品的ITS序列被分成了两大组,鸡东,汪清,东京城,东宁由于序列比较相似被分为一组,穆棱和海林被分为另一组。每组的ITS序列变化较小,相似度较高。同时,多个位点存在着差异,有转换、颠换和缺失。     

浓香型白酒酿造环节中产酯化酶的霉菌多样性

对分离自宜宾多粮浓香型白酒酿造环节中的135株霉菌产酯化酶的能力进行了检测,发现40株菌具备产生酯化酶的能力。其中以来自窖房的最多(34株),分离自糟醅次之(4株),其余为曲药(1株)、曲房(1株)。通过对这40株霉菌开展的基于ITS nu-rDNA序列的系统发育分析,发现其分属于Penicillium、Aspergillus、Emericella、Rhizopus、Cladosporium、Mucor等6个属,以Penicillium(19株)和Aspergillus(13株)为主要类群。结果显示,宜宾多粮浓香型白酒酿造环节中具备大量产酯化酶能力的霉菌,而且这些霉菌存在较为丰富的系统发育多样性。     

木醋杆菌ITS序列分析及产纤维素条件的研究

木醋杆菌(Acetobacter xylinum)是合成纤维素能力最强的细菌,设计木醋杆菌16S-23S rDNA转录间隔区(internally transcribed spacer ITS)序列引物,对其扩增.在GenBank上的登陆号为GenBank EU196159.选择到合适的木醋杆菌ITS扩增引物,PCR扩增条件.木醋杆菌最佳培养温度28℃,通过测定纤维素产量发现最佳接种量5%,最佳碳源为葡萄糖,最佳碳源浓度为2.5%.     

短波地空通信链路电磁计算与仿真

基于短波地空通信链路复杂特性是研究短波地空通信的关键和难点等问题.介绍了短波地空通信链路特性,探讨了进行短波地空通信链路电磁计算的主要参数,并利用ITS软件系列进行短波地空链路的电磁仿真并得出计算结果.计算结果可显示到地理图上,协助拟定频率规划方案,并可得出相应的电磁性能.