Combined Plate Microbial Assay (CPMA): A 6-plate-method for simultaneous first and second level screening of antibacterial residues in meat

This paper proposes an improved high throughput microbial method for the simultaneous performance of first and second level screening for antibacterial residues in meat. It is based on growth inhibition of B. subtilis on agar medium pH 6, 7.2 and 8, of B. cereus on agar medium pH 5.9, of M. luteus on agar medium pH 8 and of E. coli on agar medium pH 7.2 (research or first level screening) and on the use of confirmatory solutions (Pase, Paba, MgSO₄) for the identification or second level screening. In kidney control samples, dialysis membranes were interposed between samples and the agar surface to both prevent the action of lysozyme and reduce false positive results. The proposed method detects β-lactams, sulfonamides, tetracyclines, aminoglycosides, macrolides and quinolones at MRL concentrations and reliably indicates the inhibitor family. Results are obtained in 18-24 h.     

Quantification of anthocyanins in black carrot extracts (<Emphasis Type="Italic">Daucus carota</Emphasis> ssp.<Emphasis Type="Italic"> sativus</Emphasis> var.<Emphasis Type="Italic"> atrorubens</Emphasis> Alef.) and evaluation of their color properties

Pigment composition of 15 black carrot cultivars (Daucus carota ssp. sativus var. atrorubens Alef.) was screened by HPLC-MS. Up to seven cyanidin glycosides, five of which were acylated with hydroxycinnamic and hydroxybenzoic acids, were identified and quantified in the roots by HPLC-DAD. Contents of individual compounds indicated great differences in the potential of anthocyanin accumulation both between different cultivars and carrots of the same cultivar. Total anthocyanin amounts ranged from 45.4 mg/kg dry matter to 17.4 g/kg dry matter. To the best of our knowledge, this is the first report on the quantification of individual anthocyanins in roots of different black carrot cultivars. The determination of color properties in the extracts under various pH conditions proved black carrot anthocyanins to be applicable as natural food colorants also for low-acid food commodities, whereas a considerable loss of color was noted under nearly neutral conditions. Additionally, relatively high saccharide contents were found in almost all cultivars which may be disadvantageous when coloring concentrates are produced from carrot roots.     

Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves

Our aim was to screen for antibacterial bioactive compounds from Lonicera japonica leaves. Staphylococcus aureus and Escherichia coli were used as the indicator bacteria. Bacteriostatic assay-guided extraction and stepwise partitioning of the samples yielded five compounds of interest. Antimicrobial activities of the compounds were determined using a disk diffusion assay. Extracts, fractions, and compounds from L. japonica leaves possessed considerable antibacterial activities against the tested bacterial strains and the most active fraction was attributed to J3B2, which primarily contained 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid. Meanwhile, five bacteriostatic constituents were isolated (3-O-caffeoylquinic acid, secoxyloganin, luteoloside, 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid), among which, secoxyloganin was isolated for the first time from leaves. The antibacterial activity of the compounds was in the order of 3,5-bis-O-caffeoyl quinic acid, 4,5-bis-O-caffeoylquinic acid, luteoloside > 3-O-caffeoylquinic acid > secoxyloganin. Our results suggested that the phenolic compounds might significantly contribute to antibacterial activity and were the most responsible for the bacteriostatic activity of L. japonica leaves.     

具有优良发酵特性的德氏乳杆菌保加利亚亚种的筛选

对分离自青海、云南及蒙古国等地区的酸牦牛奶、酸山羊奶、乳饼、酸马奶、酸驼奶和酸山羊黄牛混合奶中的22株德氏乳杆菌保加利亚亚种(Lactobacillus delbeueckii subsp.bulgaricus)进行了筛选,通过对其发酵牛乳的发酵时间,pH值,滴定酸度,黏度,游离氨基氮和脱水收缩性等发酵特性进行分析,筛选出IMAU20094等7株具有优良发酵特性的试验菌,适于进一步应用研究.     

产维生素B6乳酸菌的筛选及其鉴定

以源自内蒙古呼和浩特市婴儿粪便选出的10株乳酸杆菌为试验材料,通过微生物分析方法,筛选乳酸菌合成维生素B6能力较强的菌株并对其进行初步鉴定.结果表明,有4株菌株B25、B72、B76和N59-1合成维生素B6的能力较高,其中菌株N59-1合成维生素B6的量可高达32.62 g/100 L,菌株N59-1经鉴定为植物乳杆菌.     

微柱凝胶卡式法检测红细胞不规则抗体的效果

目的评价微柱凝胶卡式法(microtubes gel test,MGT)检测红细胞不规则抗体的效果。方法分别采用MGT和传统试管抗人球蛋白法(test tube Coombs test,TT-Coombs’T)对2011年9月～2012年12月在河北省大名县人民医院输血治疗的1 147例患者的血清样本进行不规则抗体筛查,并以TT-Coombs’T作为"金标准",对MGT进行敏感性、特异性评价;采用MGT对不规则抗体检测为阳性的样本进行进一步的抗体鉴定。结果 MGT及TT-Coombs’T测定1 147份样本不规则抗体的结果均为阳性的为10份,阴性为1 137份,阳性率为0.87%,MGT的敏感性和特异性均为100%;经MGT进一步鉴定,10份不规则抗体中Rh系统抗体9份(抗-E 5份,抗-c 2份,抗-D 1份,抗-C 1份),MNS系统抗体1份(抗-M)。结论微柱凝胶卡式法检测红细胞不规则抗体敏感性和特异性好,其操作较传统试管法简便、快捷,且操作和结果的判读易于标准化,影响因素少,更适用于临床输血前不规则抗体筛查试验。     

RetCam Ⅲ视网膜成像系统在早产儿视网膜病变筛查中的临床应用

目的通过视网膜筛查了解RetCamⅢ在早产儿视网膜病变筛查工作中的应用情况。方法应用RetCamⅢ视网膜广角成像系统对入选患儿进行视网膜筛查。结果在筛查的174例小儿中,发现早产儿视网膜病变31例以及先天性白内障2例,视网膜出血4例,视网膜渗出7例。结论 RetCamⅢ能够安全有效地应用于早产儿视网膜筛查及先天性眼疾的临床筛查,有利于小儿眼病的早发现、早诊断、早治疗。     

Criterion validity of the Holden Psychological Screening Inventory Social Symptomatology Scale in a prison sample.

The 36-item, self-report Holden Psychological Screening Inventory (HPSI; R. R. Holden, 1996) and the Psychopathy Checklist–Revised (PCL–R; R. D. Hare, 1991) were administered to 214 male, adult prison inmates in Canadian federal correctional facilities. The 12-item HPSI Social Symptomatology scale, a measure of antisocial behavior, demonstrated a large effect size in significantly differentiating between PCL–R-identified psychopaths and nonpsychopaths. HPSI scales not theoretically related to psychopathic behavior showed no such significant effects. Findings are interpreted as supporting the criterion validity of the Social Symptomatology scale and suggest that this brief, self-report screen has research and clinical merit. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

油气层保护与内外泥饼关系的实验研究

通过室内实验研究,考察了高、中、低渗透地层中内外泥饼与屏蔽式暂堵的关系,并研究了去掉外泯饼和不去掉外泥饼时,钻井液和水泥浆对岩心的综合损害。研究结果表明,对渗透率不同的地层,内外泥饼所起的作用是不一样的。外泥饼的渗透率比近井地带内泥饼的渗透率低１～３个数量级。尤其是高渗透率地层,外泥饼的作用至关重要,一旦外泥饼被破坏,屏蔽带渗透率升高,损害加重。内外泥饼越致密,就越能有效地阻止固相和液相的侵入。所     

Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat

In this paper we describe the validation and utilization of the Hybrizyme TM DELFIA immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200ng/g. The limit of detection of the assay was less than 50ng/g. When the action level for the DELFIA assay was set at 100ng/g to detect samples having 200ng/g or more PCBs, the false negative rate was estimated at 0.2% . The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10ng/ g and 20ng/g PCBs, respectively. By utilizing the DELFIA assay system to select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.