Microscopic investigation of the formation of a thermoset wheat gluten network in a model system relevant for bread making

Although the impact of heat on molecular properties of wheat gluten is well understood, changes in its microstructure have rarely been studied. Here, formation of the thermoset gluten network in a model system relevant for bread baking was studied with confocal laser scanning microscopy and protein network analysis. From 65 °C onwards, gluten converts from thick aligned protein strands in a highly branched and homogeneous network of small thin protein threads. Neither gliadin incorporation in the network nor application of aqualysin 1, the thermo-active serine peptidase from Thermus aquaticus which recently has been reported to hydrolyse gluten proteins in dough only at temperatures exceeding 80 °C, impacts on the gluten microstructure. As starch causes structure setting itself and thereby decreases protein mobility, molecular scale changes in the gluten network at temperatures exceeding 80 °C brought about by aqualysin 1 do not impact its microstructure.     

Improvement of the Characteristics of Steamed Bread by Supplementation of Recombinant alpha-L-arabinofuranosidase Containing xylan-binding domain

Current study investigated the potential to increase the activity of α-L-arabinofuranosidase (TmAra) from Thermotoga marotima on insoluble arabinoxylan (IAX) through fusion to a family 6 xylan-binding domain (CtXBD6) from Clostridium thermocellum. CtXBD6 was fused to the carboxyl terminus of TmAra, and active forms of both TmAra and TmAraCtXBD6 were produced in E. coli and purified to homogeneity for biochemical characterization. TmAraCtXBD6 had similar temperature and pH profiles to TmAra with a decrease in the thermostability and pH stability at pH 7.0–8.2. It exhibited stronger IAX-binding and hydrolytic activity than TmAra. TmAra and TmAraCtXBD6-treated IAX were added to wheat flour to investigate the rheological and textural properties of the finished product. IAX alone had significant increase in dough development time (DDT) and dough stability (DS) and decrease in the softening degree (SD), crumb firmness (CF) and specific volume (SV). On adding TmAra and TmAraCtXBD6 to treat IAX texture, SV and DS improved but decreased DDT, SD, and CF. TmAraCtXBD6 reduced CF and increased SV, and had higher springiness, cohesiveness, and crumb chewiness than TmAra. These results demonstrated that the fusion of CtXBD6 into arabinofuranosidase enables the increase in catalytic performance on insoluble AX substrates, which can be used as more efficient biocatalysts that can improve the quality of AX-rich products.     

Effects of green tea powder on the quality attributes of hard red winter wheat flour and Chinese steamed bread

The effects of green tea powder (GTP) on the properties of hard red winter (HRW) flour and Chinese steamed bread were investigated. GTP was blended with HRW flour at levels of 0.00, 1.00, 2.00, 3.00 and 4.00 g GTP/100 g wheat flour. With the addition of GTP, the maximum torque of flour became stronger. The resilience and degree of green colour of steamed bread increased, while hardness and chewiness decreased, with the addition of GTP. The addition of 1.00% GTP did not significantly affect the specific volume, but remarkably influenced the hardness and resilience of steamed bread. In steamed bread formulated with GTP, the antioxidant activity was 0.84 mm TE per g at 1.00% of GTP and increased with GTP levels. The steamed bread had a pleasant flavour, and the degree of overall liking of steamed bread increased with GTP levels to the maximum used in this work.     

Staling of potato and wheat steamed breads: physicochemical characterisation and molecular mobility

To clarify the physicochemical characterisation and water status of potato steamed bread (PSB) and wheat steamed bread (WSB) during the staling process, texture, amylopectin retrogradation, crystalline structure, water migration and microstructure were evaluated by texture analysis, differential scanning calorimetry, X-ray diffraction, nuclear magnetic resonance (¹H NMR) and scanning electron microscopy (SEM). Results showed that starch molecules in PSB and WSB were recrystallised and formed B-type starch, which had a positive correlation with hardness and thermal enthalpy. Compared to WSB, PSB had a relatively low B-type crystallinity; moreover, a smaller decrease of free water in PSB was also detected in the NMR test, which indicated that more moisture can be used to plasticise crumb, thus contributing to a softer texture of PSB. SEM revealed that the amount and size of the cracks on the interface of starch and gluten matrix in WSB increased with the prolongation of storage time.     

小麦粉出粉率对馒头品质及挥发性物质的影响

为了研究小麦粉出粉率对馒头品质及挥发性物质的影响,采用不同出粉率小麦粉为原料,研究出粉率与馒头品质及其挥发性化合物的关系,为深入研究提供依据。结果表明:出粉率为35%~70%时,馒头的硬度、凝聚性和咀嚼性等无显著差异性,出粉率为80%~97%时,随着出粉率的增加,馒头硬度、胶黏性、咀嚼性显著增加,弹性、凝聚性、回复性显著下降。电子鼻检测结果显示,不同出粉率小麦粉馒头中挥发性物质存在差异,在PA/2、T40/1、P10/2传感器上的响应强度差异显著,采用电子鼻系统可以区分出粉率70%和出粉率≤70%的馒头样品。采用固相微萃取和气相色谱质谱联用的方法,从不同出粉率小麦粉馒头中共检测到59种化合物,其中醇类9种,醛类9种,呋喃类2种,酮类2种,酯类6种,苯类3种,烷烃类28种。随着出粉率增加,馒头挥发性化合物种类增加。不同出粉率小麦粉馒头中醇类物质含量最高(52.52%~65.79%),其次为醛类。     

海藻酸丙二醇酯对面团流变学和面包烘焙特性的影响

采用粉质仪、拉伸仪和质构分析仪研究添加不同比例(0.1%、0.2%、0.3%和0.4%)的海藻酸丙二醇酯(PGA)对面团流变学特性和面包烘焙特性的影响。结果表明,添加PGA能改善面团的粉质和拉伸特性,能显著提高面团的吸水率,延长面团的稳定时间,提高粉质评价指数,增大面团的延展性、拉伸阻力和拉伸比值。添加PGA能增大面包比容,显著提高面包的弹性,降低面包硬度,改善面包的口感,提高面包的感官评分。PGA添加量在0.2%~0.3%时,对面团和面包的改善效果最为显著,面包品质最好。     

大豆馒头的研究

利用现代化微生物发酵技术,将筛选出的能发酵大豆粉的优良酵母菌株,采用老面发酵的方法对全脂大豆粉∶面粉=4∶6混合粉发酵做成馒头。此馒头色泽金黄、口感细腻、香气浓厚、营养丰富,同普通馒头相比蛋白质含量增加了6.69%、铁含量增加了0.013%、钙含量增加了0.052%、磷含量增加了0.132%。     

Effect of Flour Quality, Ascorbic Acid, and DATEM on Dough Rheological Parameters and Hearth Loaves Characteristics

ABSTRACT: The effects of protein quality, protein content, ascorbic acid, diacetyl tartaric acid ester of monoglyc-erides (DATEM), and their interactions on dough rheology and hearth bread properties were studied by size-exclusion fast protein liquid chromatography, Kieffer Dough & Gluten Extensibility Rig, and small-scale baking of hearth loaves. The effect of protein content was either positive or negative on hearth loaf characteristics, form ratio, and area, depending on the amount of the largest glutenin polymers in the flour. Ascorbic acid brought out the potential in the wheat flour known as protein quality. Ascorbic acid and DATEM strengthened the doughs and improved hearth bread characteristics.