全文获取类型
收费全文 | 22469篇 |
免费 | 2265篇 |
国内免费 | 494篇 |
专业分类
电工技术 | 25篇 |
综合类 | 778篇 |
化学工业 | 6625篇 |
金属工艺 | 170篇 |
机械仪表 | 322篇 |
建筑科学 | 142篇 |
矿业工程 | 44篇 |
能源动力 | 131篇 |
轻工业 | 12705篇 |
水利工程 | 20篇 |
石油天然气 | 2440篇 |
武器工业 | 1篇 |
无线电 | 241篇 |
一般工业技术 | 808篇 |
冶金工业 | 216篇 |
原子能技术 | 51篇 |
自动化技术 | 509篇 |
出版年
2024年 | 211篇 |
2023年 | 513篇 |
2022年 | 1387篇 |
2021年 | 1497篇 |
2020年 | 906篇 |
2019年 | 985篇 |
2018年 | 795篇 |
2017年 | 898篇 |
2016年 | 815篇 |
2015年 | 919篇 |
2014年 | 1133篇 |
2013年 | 1433篇 |
2012年 | 1428篇 |
2011年 | 1426篇 |
2010年 | 1053篇 |
2009年 | 952篇 |
2008年 | 877篇 |
2007年 | 1143篇 |
2006年 | 1059篇 |
2005年 | 905篇 |
2004年 | 775篇 |
2003年 | 604篇 |
2002年 | 538篇 |
2001年 | 397篇 |
2000年 | 328篇 |
1999年 | 350篇 |
1998年 | 247篇 |
1997年 | 180篇 |
1996年 | 230篇 |
1995年 | 225篇 |
1994年 | 209篇 |
1993年 | 158篇 |
1992年 | 141篇 |
1991年 | 95篇 |
1990年 | 70篇 |
1989年 | 76篇 |
1988年 | 55篇 |
1987年 | 54篇 |
1986年 | 36篇 |
1985年 | 40篇 |
1984年 | 27篇 |
1983年 | 9篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1980年 | 29篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
51.
雷斯 《石油化工腐蚀与防护》2006,23(5):35-37,41
文章介绍了中国石油化工股份有限公司齐鲁石化分公司胜利炼油厂第二常减压蒸馏装置的总体情况,分析总结了装置在2000-2005年期间停工闲置及开工运行过程中设备腐蚀情况和防护中所做的工作,可供相似类型、相似状态的装置借鉴。 相似文献
52.
53.
为了研究遗传密码子对表达调控的影响,利用PCR重叠延伸法,对萝卜抗真菌蛋白Rs-AFP2基因编码序列区的部分核苷酸进行沉默突变,构建突变体Rs-AFPm.序列分析表明,PCR产物全长240bp,有一个阅读框,编码的蛋白由29个氨基酸的信号肽和51个氨基酸的抗真菌蛋白组成.突变体与突变前的Rs-AFP2基因相比,在编码区第3号氨基酸Lys相差一个碱基(TTG→TTA),第5号氨基酸Gln相差一个碱基(CAG→CAA),第6号稀有密码子Arg相差两个碱基(CAG→CGA).重新合成引物,将切除信号肽的Rs-AFP2基因和Rs-AFPm基因与原核表达载体pET-21b(+)分别重组到大肠杆菌BL21菌株.IPTG诱导后,二者均得到了表达.软件分析显示,突变前pETAFPo表达产物占全菌蛋白的3%,突变后pETAFPm的表达产物占全菌蛋白含量的8%;表达蛋白主要以包涵体的形式存在,包涵体经超声波破碎后,蛋白质复性,抑菌结果表明,pETAFPm表达产物的抑菌半径大于pETAFP2表达产物的抑菌半径.这些都说明改造后的Rs-AFPm基因与Rs-AFP2基因相比,已有效地提高表达量. 相似文献
54.
G
V Vijaya T Gireesh Gajanan S Bhat 《Journal of the science of food and agriculture》2002,82(5):493-496
The effect of enzymatic hydrolysis of proteins in milk using neutrase on the growth of the probiotic strain Bifidobacterium bifidus was evaluated by estimation of microbial growth, acidity, viscosity and flavour production. A significant increase in the growth of B bifidus was observed in neutrase‐hydrolysed milk. The setting time of bifidus‐cultured milk was advanced by about 12 h at 5% degree of hydrolysis. Enzymatic hydrolysis of proteins prior to cultivation also significantly increased the viscosity of the product. An approximately 60% increase in viscosity of the product was observed in neutrase‐hydrolysed milk. Production of steam‐volatile monocarbonyls as an indication of development of flavour was also higher in neutrase‐hydrolysed milk. The concentration of steam‐volatile monocarbonyls was 2.47 µmol per 100 ml in neutrase‐hydrolysed milk but only 1.84 µmol per 100 ml in control milk at the setting point of the curd. © 2002 Society of Chemical Industry 相似文献
55.
Robben J.; der Schueren J.Van; Verhasselt P.; Aert R.; Volckaert G. 《Protein engineering, design & selection : PEDS》1995,8(2):159-165
The deletion of nine residues from the C-terminus of the bacterialchloramphenicol acetyltransferase (CAT) results in depositionof the mutant protein in cytoplasmic inclusion bodies and lossof chloramphenicol resistance in Escherichia coli. This foldingdefect is relieved by C-terminal fusion of the polypeptide withas few as two residues. Based on these observations, efficientpositive selection for the cloning of DNA fragments has beendemonstrated. The cloning vector encodes a C-terminally truncatedCAT protein. Restriction sites in front of the stop codon allowthe insertion of target DNA, resulting in the production ofproperly folded CAT fusion proteins and regained chloramphenicolresistance. The positive selection of recombinants is accomplishedby growth of transformants on chloramphenicol-containing agarplates. The method appears particularly convenient for the cloningof DNA fragments amplified by the PCR because minimal informationto restore CAT folding can be included in the primers. The cloningof random sequences shows that the folding defect can be relievedby fusion to a wide variety of peptides, providing great flexibilityto the positive selection system. This vector may also contributeto the determination of the role of the C-terminus in CAT folding. 相似文献
56.
57.
高含蜡原油管道停输后的再启动问题非常复杂。它涉及因素多,问题的非线性很强。而有足够多神经元的S型三层神经网络理论上可逼近任意有理函数。文章采用误差反向传播网络预测环道实验装置中的启动压力,得到了较好结果。 相似文献
58.
天然多糖类/蛋白质复合材料的研究进展 总被引:5,自引:0,他引:5
在参考了 31篇文献的基础上 ,详述了天然多糖类与蛋白质复合材料的研究进展 相似文献
59.
ABSTRACT: :
Soybeans ( Glycine max ) were soaked and ground to obtain soymilk. The soymilk was cooked in an open tank and held at 85 to 90 deg;C. Yuba films were picked up in 20 min intervals and dried for 20 min. Yuba films were soaked in chicken-flavor solutions (25% and 35%), and baking soda (BS) solutions (0%, 1%, 2%, and 3% BS), and cooked at 100 °C for 30 min, 60 min, and 90 min. TIA decreased (p < 0.05) with the increase of heating time and BS concentration. In vitro protein digestibility (IVPD) decreased with heating time and BS concentration (p < 0.05). Sensory characteristics were affected by flavor concentration. By using 0% BS, 25% of the chicken flavor concentration, and a short heating time method, meat-like products with low TIA, high IVPD, and good sensory characteristics were obtained. 相似文献
Soybeans ( Glycine max ) were soaked and ground to obtain soymilk. The soymilk was cooked in an open tank and held at 85 to 90 deg;C. Yuba films were picked up in 20 min intervals and dried for 20 min. Yuba films were soaked in chicken-flavor solutions (25% and 35%), and baking soda (BS) solutions (0%, 1%, 2%, and 3% BS), and cooked at 100 °C for 30 min, 60 min, and 90 min. TIA decreased (p < 0.05) with the increase of heating time and BS concentration. In vitro protein digestibility (IVPD) decreased with heating time and BS concentration (p < 0.05). Sensory characteristics were affected by flavor concentration. By using 0% BS, 25% of the chicken flavor concentration, and a short heating time method, meat-like products with low TIA, high IVPD, and good sensory characteristics were obtained. 相似文献
60.
Maribel Plascencia‐Jatomea Miguel A Olvera‐Novoa Jos Luis Arredondo‐Figueroa George M Hall Keiko Shirai 《Journal of the science of food and agriculture》2002,82(7):753-759
This study provides information on the use of shrimp head silage protein hydrolysate (SPH) as an alternative protein source for tilapia feeding. Six diets (28% protein, 12% lipid) were prepared where fishmeal protein was replaced at levels of 0 (control), 10, 15, 20, 25 and 30% with the hydrolysate. The diets were supplied to Nile tilapia fry (338 mg initial weight) stocked in plastic recirculating 20 l tanks (10 animals per tank), with three replicates per treatment. After an 8 week experimental period, fish fed the diets containing 10 and 15% SPH showed significantly better performance in terms of final body weight, weight gain (%), mean daily weight gain (mg day?1), specific growth ratio and feed conversion ratio than those fed the control diet (fishmeal as protein source) and higher‐SPH diets. It is concluded that shrimp head hydrolysate is a promising alternative protein source for tilapia feeding, improving growth ratio at dietary inclusion levels as high as 15%. In addition, the diets with added shrimp silage protein were well accepted by the fish, which avidly consumed the feed during the experiment. © 2002 Society of Chemical Industry 相似文献