全文获取类型
收费全文 | 7648篇 |
免费 | 689篇 |
国内免费 | 326篇 |
专业分类
电工技术 | 53篇 |
技术理论 | 1篇 |
综合类 | 409篇 |
化学工业 | 2564篇 |
金属工艺 | 138篇 |
机械仪表 | 172篇 |
建筑科学 | 243篇 |
矿业工程 | 67篇 |
能源动力 | 46篇 |
轻工业 | 2759篇 |
水利工程 | 57篇 |
石油天然气 | 103篇 |
武器工业 | 14篇 |
无线电 | 247篇 |
一般工业技术 | 526篇 |
冶金工业 | 532篇 |
原子能技术 | 70篇 |
自动化技术 | 662篇 |
出版年
2024年 | 66篇 |
2023年 | 173篇 |
2022年 | 789篇 |
2021年 | 699篇 |
2020年 | 284篇 |
2019年 | 241篇 |
2018年 | 218篇 |
2017年 | 235篇 |
2016年 | 261篇 |
2015年 | 351篇 |
2014年 | 395篇 |
2013年 | 470篇 |
2012年 | 436篇 |
2011年 | 510篇 |
2010年 | 367篇 |
2009年 | 379篇 |
2008年 | 384篇 |
2007年 | 376篇 |
2006年 | 335篇 |
2005年 | 333篇 |
2004年 | 257篇 |
2003年 | 221篇 |
2002年 | 167篇 |
2001年 | 131篇 |
2000年 | 73篇 |
1999年 | 68篇 |
1998年 | 69篇 |
1997年 | 56篇 |
1996年 | 60篇 |
1995年 | 47篇 |
1994年 | 37篇 |
1993年 | 29篇 |
1992年 | 25篇 |
1991年 | 27篇 |
1990年 | 17篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 13篇 |
1986年 | 5篇 |
1985年 | 15篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1980年 | 3篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1960年 | 1篇 |
排序方式: 共有8663条查询结果,搜索用时 10 毫秒
71.
特征选择和维数约简在机器学习、模式识别和数据挖掘领域是很常用的方法,它们之间也具有一定的联系,但对它们的融合应用目前很少研究,从而融合特征选择和维数约简的思路被提出.该思路融合了主成分分析方法和遗传算法,提出PGS方法,并把它应用于基因microarray数据的预测分类,取得了较好的效果. 相似文献
72.
73.
Antonino B. DAssoro Roberto Leon-Ferre Eike-Benjamin Braune Urban Lendahl 《International journal of molecular sciences》2022,23(11)
The Notch signaling pathway is an architecturally simple signaling mechanism, well known for its role in cell fate regulation during organ development and in tissue homeostasis. In keeping with its importance for normal development, dysregulation of Notch signaling is increasingly associated with different types of tumors, and proteins in the Notch signaling pathway can act as oncogenes or tumor suppressors, depending on the cellular context and tumor type. In addition to a role as a driver of tumor initiation and progression in the tumor cells carrying oncogenic mutations, it is an emerging realization that Notch signaling also plays a role in non-mutated cells in the tumor microenvironment. In this review, we discuss how aberrant Notch signaling can affect three types of cells in the tumor stroma—cancer-associated fibroblasts, immune cells and vascular cells—and how this influences their interactions with the tumor cells. Insights into the roles of Notch in cells of the tumor environment and the impact on tumor-stroma interactions will lead to a deeper understanding of Notch signaling in cancer and inspire new strategies for Notch-based tumor therapy. 相似文献
74.
75.
Rui Li Yan Zhao Zhen Sun Zhenying Wu Honglun Wang Chunxiang Fu Hongbo Zhao Feng He 《International journal of molecular sciences》2022,23(12)
Plant laccase genes belong to a multigene family, play key roles in lignin polymerization, and participate in the resistance of plants to biotic and abiotic stresses. Switchgrass is an important resource for forage and bioenergy production, yet information about the switchgrass laccase gene family is scarce. Using bioinformatic approaches, a genome-wide analysis of the laccase multigene family in switchgrass was carried out in this study. In total, 49 laccase genes (PvLac1 to PvLac49) were identified; these can be divided into five subclades, and 20 of them were identified as targets of miR397. The tandem and segmental duplication of laccase genes on Chr05 and Chr08 contributed to the expansion of the laccase family. The laccase proteins shared conserved signature sequences but displayed relatively low sequence similarity, indicating the potential functional diversity of switchgrass laccases. Switchgrass laccases exhibited distinct tissue/organ expression patterns, revealing that some laccases might be involved in the lignification process during stem development. All five of the laccase isoforms selected from different subclades responded to heavy metal. The immediate response of lignin-related laccases, as well as the delayed response of low-abundance laccases, to heavy-metal treatment shed light on the multiple roles of laccase isoforms in response to heavy-metal stress. 相似文献
76.
77.
78.
79.
随着乳胶产品的广泛应用,如何鉴定乳胶中的天然成分以及对毒害化学物质的风险监控成为关注的焦点.采用红外光谱法和水提蛋白质法,利用天然乳胶中所含蛋白质的特征,可准确、快速地鉴定天然乳胶与合成聚异戊二烯乳胶.同时分析了乳胶生产过程中存在的危害化学物质风险及检测限量. 相似文献
80.
Guixiang Tang Xuanbo Zhong Wei Hong Jianfei Li Yue Shu Lulu Liu 《International journal of molecular sciences》2022,23(12)
Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2. 相似文献