Ultra-Stretchable Monofilament Flexible Sensor with Low Hysteresis and Linearity based on MWCNTs/Ecoflex Composite Materials

Wearable human–computer interaction equipment is a common technology, which can improve the comfort, convenience, and safety of the human body, and also can monitor human health. The flexible and wearable tensile sensor can be conveniently installed on clothes or directly connected to the body. This provides a convenient, timely, and portable solution for the detection of human motion. Therefore, wearable electronic equipment is gaining more attention. In this paper, a highly stretchable, flexible, and sensitive strain sensor which is based on multi-walled carbon nanotubes/Ecoflex nanocomposites is reported. A monofilament tensile sensor obtains good linearity (10.77%), low hysteresis (1.63%), good stability (6000 cycles under 100% strain), and ultra-high strain range (ε = 1300%). This ultra-stretchable sensor has potential applications in human motion monitoring, medical rehabilitation, health monitoring, human–computer interaction, and soft robots.     

聚乙二醇化重组人生长激素的性质及活性

目的　研究聚乙二醇化重组人生长激素的性质及活性。方法　通过分子筛高效液相 (SizeexclusionHPLC)、毛细管等电聚焦 (CIEF)、圆二色谱 (CD)以及荧光光谱等方法检测单聚PEG- GH的纯度、等电点、二级结构以及分子构象 ;采用妊娠兔肝细胞放射受体分析法 (RRA)检测单聚PEG GH的体外受体亲和力 ,采用未成年去垂体大鼠体重增加法检测体内生物活性。结果　分子筛高效液相图谱上仅出现一个峰 ,表明样品纯度很高 ,等电点范围为 5 .1～ 5 .2 ,与未修饰的rhGH的等电点相一致 ;CD谱与荧光光谱均表明单个mPEG ALD的修饰对rhGH的二级结构以及分子构象没有明显影响 ;与未修饰的rhGH相比 ,单聚PEG GH体外受体亲和力明显降低 ;但体内作用时间更长 ,且更加有效。结论　此结果为进一步研究和开发长效重组人生长激素提供了理论依据。     

重组人EC-SOD包涵体的稀释复性及重折叠后蛋白的纯化

目的确定重组人ECSOD包涵体体外稀释复性和复性后蛋白纯化的适宜方法。方法通过对pH、温度、包涵体蛋白浓度、尿素和精氨酸浓度及氧化还原对比率的定性定量分析,研究重组人ECSOD包涵体体外稀释复性的基本条件。采用金属离子亲和柱(IMAC)和肝素亲和柱(Heparinsepharose)对复性蛋白进行纯化,比较纯化效率的差异。结果最佳复性条件为pH8.5,尿素浓度为1.5molL,精氨酸浓度大于0.3molL,GSH∶GSSG=4∶1;复性温度及包涵体蛋白终浓度越高,复性效率越低。IMAC和Heparinsepharose亲和柱均可用来纯化复性蛋白溶液,但Heparinsepharose纯化效率较高。结论确定了重组人ECSOD包涵体体外稀释复性的最佳条件及复性后蛋白纯化的方法。     

重组人白细胞介素-10在大肠杆菌中的表达及生物学活性分析

目的在大肠杆菌中表达重组人白细胞介素-10(Interleukin-10,IL-10),并检测其生物学活性。方法将IL-10基因重组到质粒pET11c中,转化BL21(DE),提取质粒,经酶切鉴定和测序分析;在25℃用低浓度的IPTG诱导表达,对包涵体IL-10稀释复性;经ELISA检测其含量,MC/9细胞增殖法检测其生物学活性。结果工程菌IL-10/pET11c/BL21诱导表达的目的蛋白以可溶性和包涵体两种形式存在,Westernblot鉴定证实为IL-10蛋白,两种形式的IL-10均具有一定的生物学活性。结论已成功地在大肠杆菌中表达了IL-10,为进一步纯化和制备IL-10的基因工程药物打下了基础。     

仿人智能橡胶制品的精密硫化控制

分析了橡胶制品硫化成型的对象特性，选择了硫化成型过程控制策略，研究了控制算法及其工程应用。研究结果表明，选择仿人智能控制作为硫化成型过程的控制策略时，可实现速度快、精度高的控制，且具有极强的鲁棒性。     

50年来辽宁省不同等级舒适日数演变及空间区划分析

根据1964—2015年辽宁省23个气象站点的逐日平均气温、相对湿度及平均风速资料,采用人体舒适度指数法计算人体舒适度指数,在季尺度上分析辽宁省不同等级舒适日数演变情况及典型旅游城市最佳旅游期;运用旋转经验正交函数分解法(REOF),分析人体舒适度指数季尺度空间变化特征,并应用ArcGIS软件进行舒适区域划分。结果表明:①辽宁省多年平均冷不舒适日数呈降低趋势,舒适日数呈增加趋势,热不舒适日数也呈微弱增加趋势;舒适程度夏季＞秋季＞春季＞冬季;②除冬季外5座典型旅游城市均适宜旅游,夏季为最佳旅游期;其中,沈阳、本溪更适宜春季旅游,大连更适宜秋季旅游。③辽宁省四季舒适度变化情况可大致划分为辽西、辽中、辽东三个区域,具体划分情况随季节有相应变化,总体舒适度由辽宁东部向西部逐渐递减。     

Utility of stereoscopic displays for indirect-vision driving and robot teleoperation

The effectiveness of an active shutter-glasses stereoscopic display (SD) and a passive polarised SD was evaluated in a live robot-teleoperation task and a simulated indirect-vision driving task in various terrains. Overall, participants completed their tasks significantly faster with the SDs in three-dimensional (3D) mode than with the SDs in the baseline 2D mode. They also navigated more accurately with the SDs in 3D mode. When the effectiveness of the two types of SDs was examined separately, results showed that the active shutter-glasses SD resulted in faster responses and task completion times than the passive polarised SD, though most of the differences failed to reach statistical significance. Perceived workload when interacting with the two SD systems did not differ significantly between the active versus passive display types or between the 3D and 2D modes of operation; however, participants reported more severe discomfort after interacting with the passive polarised SD.     

A 3D analysis of the joint torques developed during driver's ingress–egress motion

Providing an easy ingress–egress (I/E) movement remains a challenge for car designers. I/E has been largely studied in kinematics, but not in dynamics. This study proposes: (1) to evaluate and describe the motor torques developed in the lower limbs and lumbar joints during I/E motions and (2) to analyse the influence of the car geometry and subject anthropometry. An experiment was performed to observe 15 subjects of three anthropometrical groups getting in and out of a car mock-up simulating three different vehicle configurations. Motor torques were extracted using an inverse dynamics analysis. Both ingress and egress motions were primarily characterised by large torques. Overall, the taller a subject and the lower the seat of the vehicle were, the larger the peak torques were. Moreover, peak torques were higher for egress than ingress. These results are discussed in regard to the current knowledge on I/E ergonomics.     

免水型生态卫生设施用降解菌的分离筛选

本文对免水型生态卫生设施中的生物填料及施有机肥农田的土壤进行了人类粪便降解菌的分离。筛选得到的各菌种在实验条件下，降解粪便效果较好，除臭除味，COD下降率在30％左右。其中在土壤中分离出来的9号菌，COD下降率达41％，全部菌种复合培养处理的下降率高达59％。结果表明，实验采用的分离筛选方法是比较有效的，筛选得到了具有较好降解效果的菌种，为降解菌的进一步试验打下了基础。     

Recommending suitable learning scenarios according to learners’ preferences: An improved swarm based approach

The paper presents a new approach for recommending suitable learning paths for different learners groups. Selection of the learning path is considered as recommendations to choosing and combining the sequences of learning objects (LOs) according to learners’ preferences. Learning path can be selected by applying artificial intelligence techniques, e.g. a swarm intelligence model. If we modify and/or change some LOs in the learning path, we should rearrange the alignment of new and old LOs and reallocate pheromones to achieve effective learning recommendations. To solve this problem, a new method based on the ant colony optimisation algorithm and adaptation of the solution to the changing optimum is proposed. A simulation process with a dynamic change of learning paths when new LOs are inserted was chosen to verify the method proposed. The paper contributes with the following new developments: (1) an approach of dynamic learning paths selection based on swarm intelligence, and (2) a modified ant colony optimisation algorithm for learning paths selection. The elaborated approach effectively assist learners by helping them to reach most suitable LOs according to their preferences, and tutors – by helping them to monitor, refine, and improve e-learning modules and courses according to the learners’ behaviour.