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81.
J G De Nobel F M Klis A Ram H Van Unen J Priem T Munnik H Van Den Ende 《Yeast (Chichester, England)》1991,7(6):589-598
To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease. 相似文献
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84.
本文通过对蜂窝陶瓷载体成型过程的分析,指出在其成型过程中存在的问题,提出了采用液压同步剪解决这些问题的方法。论文分析了同步剪的工作原理,给出了其液压原理图及其设计特点。 相似文献
85.
大型结构物的称重通过多个千斤顶同步顶升实现。大型结构物往往存在柔度大、重量分布不均、支点跨距大的特点。在称重过程中需要进行计算机同步控制,实现结构物的平稳顶升和下降。大型结构物自动称重以工控机为核心,基于现场总线技术,对压力传感器、位移传感器、状态设置开关、油泵换向阀和油管超高压电磁阀等设备进行网络监控,对多个支点同步协调控制和结构物的精确称重,该系统已在多个油气田工程的建造中得到实际应用。 相似文献
86.
介绍了利用高炉鼓风机基础实施不易地扩容改造的设计工艺方法,该方法不仅降低了设计、施工、安装费用,而且缩短了施工周期,降低了固定资产投资。 相似文献
87.
Akitoshi Iwata Shinji Ichikawa Mutuwo Tomita Shinji Doki Shigeru Okuma 《Electrical Engineering in Japan》2006,155(3):62-69
This paper presents a novel on‐line parameter identification method for sensorless control of Synchronous Reluctance Motors (SynRMs). Although conventional sensorless control methods based on mathematical models usually need some complex measurements of motor parameters in advance, the proposed identification method does not require them and can be realized on‐line. The proposed method identifies motor parameters under sensorless control, so rotor position and velocity cannot be used to identify these parameters. However, the proposed method does not need rotor position and velocity, and identified parameters are not affected by these estimation errors. The sensorless control using identified motor parameters is realized, and the effectiveness of the proposed method is verified by experimental results. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(3): 62–69, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20258 相似文献
88.
介绍了盘式永磁无刷电动机的原理和结构,探讨了其设计特点,解决了磁路分析计算和电机主要尺寸确定以及磁极形状选择的问题。 相似文献
90.
Kallie Christopher S.; Schrater Paul R.; Legge Gordon E. 《Canadian Metallurgical Quarterly》2007,33(1):183
Walking without vision results in veering, an inability to maintain a straight path that has important consequences for blind pedestrians. In this study, the authors addressed whether the source of veering in the absence of visual and auditory feedback is better attributed to errors in perceptual encoding or undetected motor error. Three experiments had the following results: No significant differences in the shapes of veering trajectories were found between blind and blindfolded participants; accuracy in detecting curved walking paths was not correlated with simple measures of veering behavior; and explicit perceptual cues to initial walking direction did not reduce veering. The authors present a model that accounts for the major characteristics of participants' veering behavior by postulating 3 independent sources of undetected motor error: initial orientation, consistent biases in step direction, and, most important, variable error in individual steps. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献