大豆异黄酮在军用功能食品中的应用

介绍了大豆异黄酮的基本概念及生物学功能,并列举了其在军用抗疲劳功能食品、军用耐缺氧功能食品、军用抗辐射功能食品、预防骨质疏松功能食品中的应用,并对大豆异黄酮在军用功能食品中的应用作一展望。     

Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria lobata Ohwi)

ABSTRACT: Isoflavones and their glycosides were isolated from kudzu root, and formononetin-7- O -glucoside (ononin) was identified from the plant for the first time. Among isolated compounds, the antioxidant potency of 5 major compounds was determined using (1) 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay, (2) thiocyanate assay in the linoleic acid model system, and (3) lipoxygenase inhibition assay. The study indicates that 5 compounds act as free-radical scavengers and inhibit both linoleic acid peroxidation and lipoxygenase activity. The 3 different assays for determining antioxidant activity revealed that activity in one test did not necessarily correlate with activity in another test. Arachidonic acid release in the intact HL-29 cancer cell, biochanin A, showed strong inhibition of about 70%, at a concentration of 50 μ.M.     

微波法预处理提取大豆异黄酮的研究

本研究以低温脱脂豆粕为原料，利用微波对其进行前处理，以提高浸提效率。试验结果表明，适当的微波处理不但可以提高大豆异黄酮的溶出率，同时可提高提取操作的选择性，是一种有效的处理方法。     

大孔吸附树脂吸附大豆异黄酮的特性研究

以分离和提纯大豆异黄酮为目的 ,研究了AB 8、NKA 9,DM 30 1三种大孔吸附树脂对大豆异黄酮的吸附特性。结果表明 :AB 8树脂在低温下有利于对大豆异黄酮的吸附 ,3种树脂的平衡吸附量依次为AB 8>DH 30 1 >NKA 9。另外 ,AB 8树脂固定床的吸附穿透曲线表明 ,高浓度样液以较低的流速通过树脂层可以提高动态吸附的吸附速率。AB 8树脂对吸附的大豆异黄酮洗脱量大而且速度快 ,解吸容易 ,其次是DM 30 1树脂 ,而NKA 9树脂不仅洗脱速率低 ,而且耗费洗脱液。     

无机膜超滤法制备大豆异黄酮的研究

用80%乙醇水溶液提取大豆异黄酮,比较孔径为50 nm和10 nm的无机陶瓷膜在0.13MPa下超滤大豆乙醇提取液的效果,制备较高纯度的大豆异黄酮。超滤结果表明,超滤可以脱除69.4%胶质,66%蛋白质;滤过液色泽变浅,澄清度显著提高;浸提液中大豆异黄酮的组成及含量在超滤前后没有明显变化。冷冻干燥得到大豆异黄酮,纯度可达23.6%。     

盐酸水解大豆异黄酮的工艺研究

研究了用酸水解大豆异黄酮分子上的部分糖基，使异黄酮部分水解生成低糖，高活性苷元的方法。并通过HPLC分析得到大豆异黄酮酸解最佳反应条件为：0.1mol/L盐酸：时间3h；温度40℃；盐酸体积原料比5:1。金雀异黄素含量5.95%，分解率为5.25%。     

大豆异黄酮的研究进展

本文综述了近年来对大豆异黄酮的研究进展。品种、栽培环境、加工工艺等条件对大豆及其食品中的异黄酮的含量与种类均有影响。动物实验、临床研究以及流行病学调查表明,大豆异黄酮具有很强的抗癌活性以及预防骨质疏松症、更年期综合症、心血管疾病等生理功能。     

大孔树脂对豆粕中异黄酮吸附特性的研究

选用高效液相色谱法及紫外分光光度法检测溶液中大豆异黄酮的含量。比较3种不同型号大孔树脂对大豆异黄酮的吸附解吸性质，从中筛选出效果较好的ADS-21型树脂进行静态及动态动力学特性研究，得到该树脂在室温条件下对大豆异黄酮的吸附及解吸动力学曲线。     

Isoflavone Conversion of Black Soybean by Immobilized Rhizopus spp.

Isoflavones exhibit many bioactive benefits, such as antioxidation, antimutagenic effects, and reduction of symptoms of postmenopause conditions. Aglycone forms of isoflavone have shown more biological effects over their glycosylated ones. Most isoflavones in nature, however, exist as glycosylated forms and cannot easily be absorbed in the intestines. The purpose of this study was to develop a fermentation process of black soybean to produce functional food using a Luffa cylindrical fiber biofilm reactor. Three filamentous-fungi, including Rhizopus oligosporus (BCRC 31996), Rhizopus oligosporus (NTU-5), and Rhizopus oryzae (BCRC 30894), were cultivated in black soybean medium. The assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect of fermented broth and isoflavone aglycosylation rate of genistin and daidzin were evaluated as indicators to determine the most suitable strain. After a 2-day cultivation in flasks, DPPH radical scavenging effect of R. oligosporus NTU-5 reached 83%, which is the maximum value among three strains. R. oligosporus NTU-5 was subsequently chosen as target strain and used for the scale-up of fungal-based aerobic growth. The optimal cultivation condition obtained in a 5-L bioreactor with Luffa cylindrical fiber was 30°C, 0.1 vvm aeration, 100 rpm agitation, 10% inoculum volume, and 6% (w/v) black soybean as medium. After a 6-day cultivation, R. oligosporus NTU-5 converted isoflavones to their aglycones, daidzein and genistein, and these were 59.7 and 23.6 μM, respectively. Isoflavone aglycosylation rate of daidzin and genistin were 77.6 and 31.8%, respectively. The DPPH scavenging effect was 62%.     

大豆异黄酮的纯化工艺研究

采用大孔吸附树脂、硅胶、葡聚糖凝胶及反相硅胶RP-18等4种填料的柱层析法纯化大豆异黄酮。结果表明,四种层析填料的纯化效果均较好,大豆异黄酮纯度达30％以上,其中以葡聚糖凝胶为填料的柱层析法分离纯化效果最佳,纯度可达55．45％,提取率为90％。