Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats

Transplantation of various types of stem cells as a possible therapy for stroke has been tested for years, and the results are promising. Recent investigations have shown that the administration of the conditioned media obtained after stem cell cultivation can also be effective in the therapy of the central nervous system pathology (hypothesis of their paracrine action). The aim of this study was to evaluate the therapeutic effects of the conditioned medium of hiPSC-derived glial and neuronal progenitor cells in the rat middle cerebral artery occlusion model of the ischemic stroke. Secretory activity of the cultured neuronal and glial progenitor cells was evaluated by proteomic and immunosorbent-based approaches. Therapeutic effects were assessed by overall survival, neurologic deficit and infarct volume dynamics, as well as by the end-point values of the apoptosis- and inflammation-related gene expression levels, the extent of microglia/macrophage infiltration and the numbers of formed blood vessels in the affected area of the brain. As a result, 31% of the protein species discovered in glial progenitor cells-conditioned medium and 45% in neuronal progenitor cells-conditioned medium were cell type specific. The glial progenitor cell-conditioned media showed a higher content of neurotrophins (BDNF, GDNF, CNTF and NGF). We showed that intra-arterial administration of glial progenitor cells-conditioned medium promoted a faster decrease in neurological deficit compared to the control group, reduced microglia/macrophage infiltration, reduced expression of pro-apoptotic gene Bax and pro-inflammatory cytokine gene Tnf, increased expression of anti-inflammatory cytokine genes (Il4, Il10, Il13) and promoted the formation of blood vessels within the damaged area. None of these effects were exerted by the neuronal progenitor cell-conditioned media. The results indicate pronounced cytoprotective, anti-inflammatory and angiogenic properties of soluble factors secreted by glial progenitor cells.     

热管空冷器在淬火介质控温过程中的应用

针对连续淬火介质在工作中存在的冷却问题，提出由空气冷却代替水冷系统，研制出的热管空冷器可以精确控制连续淬火介质温度，保证热处理工件的金相组织和力学性能，在实际应用中取得较大成效。     

冷却介质对ZG30CrMn2Si2NiMo组织和力学性能的影响

研究了不同冷却介质对ZG30CrMn2Si2NiMo铸钢力学性能及组织的影响.试验结果表明,奥氏体化后空冷ZG30CrMn2Si2NiMo组织为板条贝氏体型铁素体和残余奥氏体组织,奥氏体化后淬火ZG30CrMn2Si2NiMo组织为板条马氏体、贝氏体型铁素体和残余奥氏体组织.ZG30CrMn2Si2NiMo获得板条马氏体、贝氏体型铁素体和残余奥氏体组织时,具有良好的力学性能.     

利用"正火液"作为高铬铸铁磨球淬火介质的生产实践

高铬铸铁件在没有添加贵重元素的条件下,利用"正火液"配入适量的水作为淬火介质进行了试验和生产.实践证明,产品在热处理淬火时从未发生炸裂现象,同时完全达到了要求的力学性能指标.生产的锤头其使用寿命为高锰钢的6～8倍,为低铬白口铸铁的4～6倍.     

采用中频炉冶炼LCC低温钢的实践与应用

介绍了中频炉炼LCC钢时 ,通过选择合适的炉衬和包衬材料 ,严谨的操作工艺 ,降低钢水中的气体和夹杂 ,从而使LCC钢低温性能合格率大大提高     

高密度钨合金热静液挤压流动粘度的数值计算

采用粘性流体的运动微分方程分析工作介质在锥模中的挤压流动，通过分析工作介质的粘度对热静液挤压的影响，指出了合理的选择工作介质的成分使得粘度适宜，可以使热静液挤压力最小，而且能够避免挤压件缺陷的产生。挤压介质的粘度是关系到坯料变形流动的润滑性能及挤压变形流动性能的关键。在此基础上，给出了挤压介质的粘度的数学关系式，为热静液挤压工艺奠定重要的理论基础。     

我国型钢生产技术进步20年及展望

按小型材、棒线材、中型材、轨梁和大型材分类 ,对 2 0年来我国在型钢生产领域的主要技术进步进行了总结 ,对其生产现状进行了分析 ,对其发展前景进行了展望     

磁性材料产业现状与展望

介绍磁性材料的主要品种，如永磁体、软磁体和磁记录介质材料的行业现状及技术进步。     

Preparation of solar selective absorbing CuO coating for medium temperature application

A new method of preparing CuO solar selective absorbing coating for medium temperature is presented. After pretreatment, brass was overlaid with CuO by chemical plating. The effects of reactant concentration, reaction temperature and reaction time on the absorptivity of CuO coating were investigated. The optimized condition of preparing CuO coating was obtained. The CuO coating was analyzed with X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). In order to prolong the period of use, the CuO coating was protected by TiO₂. The experiment shows that the TiO₂/CuO coating is more heat-resistant, acid-resistant, and wear resistant than CuO coating, without losing absorptivity markedly. The TiO₂ coating can reduce emissivity and protect the CuO coating. Translated from Journal of Tianjin University, 2006, 39(12): 1485–1489 [译自: 天津大学学报]     

火焰原子吸收光谱法测定高纯石英粉中痕量钙

系统地研究了用空气——乙炔火焰原子吸收光谱分析法测定高纯石英粉中钙的最佳条件，建立了14％的乙醇作为增感剂，在2％盐酸介质中，0．4％ LaCl3溶液作为释放剂，用塞曼火焰原子吸收法测定的可行性方案，探讨了共存元素对钙的干扰及其消除。方法操作简便快速，干扰少，方法相对标准偏差小于3％，适用于日常分析石英粉中的杂质。