Multi-Component HPLC Analysis and Antioxidant Activity Characterization of Extracts from Dipsacus sativus (Linn.) Honck

A highly reliable high-performance liquid chromatography method was developed and validated for determining quality of extracts from the leaves of Dipsacus sativus (Linn.) Honck. Under optimum conditions, the chromatography profile of eight compounds was collected, with compound saponarin as marker. The analysis results indicated that saponarin and isovitexin were the main active components. Pharmacological experiments proved that extracts from the leaves showed significant antioxidant activity. This work could serve to validate the usefulness of high-performance liquid chromatography analyses in traditional Chinese medicine.     

黄兰挥发油的化学成分研究

采用同时蒸馏萃取法(SDE)分别提取6月份采摘的黄兰叶、花和果实的挥发油,运用毛细管气相色谱-质谱联用法结合计算机检索对其挥发油进行了化学成分分析。分别从黄兰叶、花和果实的挥发油中鉴定出了17、35和38种化合物。用面积归一化法测定了3种挥发油中各种成分的相对质量分数,各占总峰面积的92.18%、99.23%和99.24%。3种挥发油化学组成各有异同,主要成分有β-芳樟醇、大牻牛儿烯B、石竹烯、桉叶醇、β-榄香烯等。黄兰挥发油中富含大量在香料和医药行业有重要用途的高生物活性化合物。     

樟树果红色素的提取及性质研究

研究了樟树果红色素的提取条件和理化性质。结果表明, pH值1.0时的20%乙醇是樟树果红色素的最佳提取剂;樟树果红色素属花青素类色素,易溶于水和酸性乙醇;pH值对色素影响明显,在酸性条件下色泽稳定且具有热稳定性。光照能加快色素降解。金属离子Na⁺、Ca²⁺、Al³⁺、Cu²⁺、Zn²⁺对色素色泽无影响,而Fe³⁺、Pb²⁺有不良影响。色素的抗氧化性能较差而抗还原性能较好。蔗糖、葡萄糖和盐等添加剂对色素无影响。     

Structure and Antioxidant Activities of Proanthocyanidins from Elephant Apple (Dillenia indica Linn.)

Proanthocyanidins were isolated and purified from fruits of elephant apple (Dillenia indica Linn.) and their structural and bioactive properties were examined. Bate‐Smith alcoholysis, FTIR, and ¹³C NMR spectra revealed that elephant apple proanthocyanidins (EAPs) contained a dominant amount of B‐type procyanidins (PC) with a minor amount of B‐type prodelphinidins (PD) but no A‐type interflavan linkage. ¹³C NMR spectrum indicated that the cis isomer was dominant in EAPs. The electron spray ionization and matrix‐assisted laser desorption ionization time of flight mass spectra of EAPs showed the clear ion peaks corresponding to B‐type PC dimer to B‐type PD with degree of polymerization of 11. EAPs had strong antioxidant activity, which was evidenced by the high oxygen radical scavenging capacity at 1.06 × 10⁴ μmol TE/g and ferric reducing antioxidant power of 2320 μmol Fe(II)/g. The results suggest that EAPs could be extracted to be used as promising functional food materials.     

响应面法优化槐花杏鲍菇酱工艺的研究

以新鲜的槐花和杏鲍菇为主要原料,研发槐花杏鲍菇酱.利用单因素试验分别研究槐花添加量、杏鲍菇添加量、大豆油添加量、辣椒添加量4个因素对槐花杏鲍菇酱感官品质的影响.在单因素试验的基础上,采用响应面法优化获得生产槐花杏鲍菇酱的最优工艺条件:槐花添加量21％、杏鲍菇添加量20％、大豆油添加量25％、辣椒添加量0.4％,在此条件...     

超临界CO2萃取沙棘油工艺条件的优化

在萃取时间为３ｈ,ＣＯ２流量为３￣４ｋｇ／ｈ条件下,按不同萃取压力和不同萃取温度、分离温度萃取沙棘籽油。试验结果表明,萃取压力为２８￣３０ＭＰａ,萃取温度为４８￣５０℃,分离温度为环境温度时,超临界ＣＯ２萃取沙棘籽油的提取工艺条件为最佳,此条件下的沙棘籽油得率可达６．８％以上。     

沙棘制取维生素试验研究

本文介绍了沙棘的生物学特性、生态学特性、生态功能及其经济价值。通过试验获取了沙棘果汁维生素C、E、A原的含量的数据,为我国沙棘的药用提供了宝贵的资料;同时,本文也为沙棘栽培和沙棘制药工作者提供了参考资料。     

The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn.

Humulus lupulus Linn. is a traditional medicinal and edible plant with several biological properties. The aims of this work were: (1) to evaluate the in vitro antifungal activity of H. lupulus ethanolic extract; (2) to study the in vitro and in vivo antifungal activity of isoxanthohumol, an isoprene flavonoid from H. lupulus, against Botrytis cinerea; and (3) to explore the antifungal mechanism of isoxanthohumol on B. cinerea. The present data revealed that the ethanolic extract of H. lupulus exhibited moderate antifungal activity against the five tested phytopathogenic fungi in vitro, and isoxanthohumol showed highly significant antifungal activity against B. cinerea, with an EC₅₀ value of 4.32 µg/mL. Meanwhile, it exhibited moderate to excellent protective and curative efficacies in vivo. The results of morphologic observation, RNA-seq, and physiological indicators revealed that the antifungal mechanism of isoxanthohumol is mainly related to metabolism; it affected the carbohydrate metabolic process, destroyed the tricarboxylic acid (TCA) cycle, and hindered the generation of ATP by inhibiting respiration. Further studies indicated that isoxanthohumol caused membrane lipid peroxidation, thus accelerating the death of B. cinerea. This study demonstrates that isoxanthohumol can be used as a potential botanical fungicide for the management of phytopathogenic fungi.     

海南红厚壳花浸膏、精油的提取及化学成分分析

分别用水蒸气蒸馏法、溶剂法以及CO2超临界萃取法,对海南红厚壳鲜花进行精油、浸膏提取。3种不同提取方法的得油率分别为0.059%、0.33%、0.20%。用GC-MS技术对其进行了化学成分分析鉴定,并比较不同提取方法所得产物化学成分的异同。水蒸气蒸馏法所得精油共鉴定出25种化合物,主要成分为w(大根香叶烯)=22.62%、w(反式-石竹烯)=12.03%、w(β-倍半水芹烯)=19.00%;石油醚浸提所得浸膏鉴定出25种化合物,主要成分为w(大根香叶烯)=21.24%、w(β-倍半水芹烯)=9.07%、w(高香叶烯)=5.69%;超临界CO2萃取所得浸膏鉴定出19种化合物,主要成分为w(大根香叶烯-D)=17.70%、w(反式-石竹烯)=15.43%、w(16-贝壳松烯)=11.55%、w(α-柯拜烯)=10.62%。     

银杏叶化学成分及其应用研究进展

银杏叶提取物广泛用于食品、保健品、化妆品和药品,是国内外开发利用的热点。本文重点介绍国内外银杏叶开发概况、银杏叶中黄酮类、萜内酯、聚戊烯醇和多糖类化合物等活性物的化学结构,及其药理作用和临床应用,为银杏叶有效成分的综合加工利用提供基础。