基于氧电极的乙醇微生物传感器的在线检测系统

乙醇氧化菌消耗利用乙醇,并产生溶解氧的特性,结合 Clark 氧电极技术设计一种新的乙醇微生物传感器检测方法.该微生物为嗜有机甲基杆菌,是以乙醇作为唯一的碳源生长的菌株.采用利于微生物细胞生长的 PVA-海藻酸盐溶胶-凝胶包埋法,对筛选、分离得到的乙醇氧化细菌固定处理,通过 Clark 氧电极对催化乙醇产生的溶解氧进行...     

Design and evaluation of a collaborative learning environment

Collaboration becomes an essential competency in the current knowledge society. In this study, a collaborative learning environment was designed to facilitate students in group collaboration. Instructional support strategies of friendship and meaningful learning tasks were applied to promote collaboration. Scaffolding strategies such as writing progress reports and developing product versions were used to coordinate and monitor the collaborative learning environment. In addition, the online file sharing tool DriveHQ was used to facilitate collaboration. Four classes of students used the learning environment to complete their final project in pairs. Results showed friendship and meaningful learning tasks helped to promote individual accountability and positive interdependence, and the use of progress reports and product versions was useful for coordinating and monitoring the learning process. However, additional strategies might be needed for more effective collaboration to take place. Issues involved in this study are discussed.     

Learning teamwork skills in university programming courses

University courses about computer programming usually seek to provide students not only with technical knowledge, but also with the skills required to work in real-life software projects. Nowadays, the development of software applications requires the coordinated efforts of the members of one or more teams. Therefore, it is important for software professionals to master the sort of skills that assure the success of teamwork, such as communication, leadership, negotiation, or team management. However, these abilities are difficult to teach, one of the reasons being that they require true commitment from the students. However, today students are taking a more and more passive role in their own education, two of the more evident consequences being the increase in dropout rates and the decrease in marks obtained in exams. The NUCLEO e-learning framework has been designed to promote the effective acquisition of teamwork skills and, at the same time, to promote the more active participation of the students in their own learning process. NUCLEO adopts a socio-constructivist pedagogical approach that pursues the development of communities of practice for Problem Based Learning. Our research has rooted the design decisions of NUCLEO in the analysis of its socio-cultural environment with Activity Theory, which considers conflicts within groups as the impetus of their evolution and the forges of their environments. This paper presents the analysis of the main features of NUCLEO according to Activity Theory, as well as the experimental results obtained with the framework in three different case studies in university courses.     

The provision of online public goods: Examining social structure in an electronic network of practice

Electronic networks of practice are computer-mediated social spaces where individuals working on similar problems self-organize to help each other and share knowledge, advice, and perspectives about their occupational practice or common interests. These interactions occur through message postings to produce an on-line public good of knowledge, where all participants in the network can then access this knowledge, regardless of their active participation in the network. Using theories and concepts of collective action and public goods, five hypotheses are developed regarding the structural and social characteristics that support the online provision and maintenance of knowledge in an electronic network of practice. Using social network analysis, we examine the structure of message contributions that produce and sustain the public good. We then combine the results from network analysis with survey results to examine the underlying pattern of exchange, the role of the critical mass, the quality of the ties sustaining participation, the heterogeneity of resources and interests of participants, and changes in membership that impact the structural characteristics of the network. Our results suggest that the electronic network of practice chosen for this study is sustained through generalized exchange, is supported by a critical mass of active members, and that members develop strong ties with the community as a whole rather than develop interpersonal relationships. Knowledge contribution is significantly related to an individual's tenure in the occupation, expertise, availability of local resources and a desire to enhance one's reputation, and those in the critical mass are primarily responsible for creating and sustaining the public good of knowledge. Finally, we find that this structure of generalized exchange is stable over time although there is a high proportion of member churn in the network.     

Estimating life-form cover fractions in California sage scrub communities using multispectral remote sensing

The California sage scrub (CSS) community type in California's Mediterranean-type ecosystems is known for its high biodiversity and is home to a large number of rare, threatened, and endangered species. Because of extensive urban development in the past fifty years, this ecologically significant community type is highly degraded and fragmented. To conserve endangered CSS communities, monitoring internal conditions of communities is as crucial as monitoring distributions of the community type in the region. Vegetation type mapping and field sampling of individual plants provide ecologically meaningful information about CSS communities such as spatial distribution and species compositions, respectively. However, both approaches only provide spatially comprehensive information but no information about internal conditions or vice versa. Therefore, there is a need for monitoring variables which fill the information gap between vegetation type maps and field-based data. A number of field-based studies indicate that life-form fractional cover is an effective indicator of CSS community health and habitat quality for CSS-obligated species. This study investigates the effectiveness of remote sensing approaches for estimating fractional cover of true shrub, subshrub, herb, and bare ground in CSS communities of southern California. Combinations of four types of multispectral imagery ranging from 0.15 m resolution scanned color infrared aerial photography to 10 m resolution SPOT 5 multispectral imagery and three image processing models - per-pixel, object-based, and spectral mixture models - were tested.An object-based image analysis (OBIA) routine consistently yielded higher accuracy than other image processing methods for estimating all cover types. Life-form cover was reliably predicted, with error magnitudes as low as 2%. Subshrub and herb cover types required finer spatial resolution imagery for more accurate predictions than true shrub and bare ground types. Positioning of sampling grids had a substantial impact on the reliability of accuracy assessment, particularly for cover estimates predicted using multiple endmember spectral mixture analysis (MESMA) applied to SPOT imagery. Of the approaches tested in this study, OBIA using pansharpened QuickBird imagery is one of the most promising approaches because of its high accuracy and processing efficiency and should be tested for more heterogeneous CSS landscapes. MESMA applied to SPOT imagery should also be examined for effectiveness in estimating factional cover over more extensive habitat areas because of its low data cost and potential for conducting retrospective studies of vegetation community conditions.     

The effect of Twitter on college student engagement and grades

Despite the widespread use of social media by students and its increased use by instructors, very little empirical evidence is available concerning the impact of social media use on student learning and engagement. This paper describes our semester‐long experimental study to determine if using Twitter – the microblogging and social networking platform most amenable to ongoing, public dialogue – for educationally relevant purposes can impact college student engagement and grades. A total of 125 students taking a first year seminar course for pre‐health professional majors participated in this study (70 in the experimental group and 55 in the control group). With the experimental group, Twitter was used for various types of academic and co‐curricular discussions. Engagement was quantified by using a 19‐item scale based on the National Survey of Student Engagement. To assess differences in engagement and grades, we used mixed effects analysis of variance (ANOVA) models, with class sections nested within treatment groups. We also conducted content analyses of samples of Twitter exchanges. The ANOVA results showed that the experimental group had a significantly greater increase in engagement than the control group, as well as higher semester grade point averages. Analyses of Twitter communications showed that students and faculty were both highly engaged in the learning process in ways that transcended traditional classroom activities. This study provides experimental evidence that Twitter can be used as an educational tool to help engage students and to mobilize faculty into a more active and participatory role.     

Prefetching in Content Distribution Networks via Web Communities Identification and Outsourcing

Content distribution networks (CDNs) improve scalability and reliability, by replicating content to the “edge” of the Internet. Apart from the pure networking issues of the CDNs relevant to the establishment of the infrastructure, some very crucial data management issues must be resolved to exploit the full potential of CDNs to reduce the “last mile” latencies. A very important issue is the selection of the content to be prefetched to the CDN servers. All the approaches developed so far, assume the existence of adequate content popularity statistics to drive the prefetch decisions. Such information though, is not always available, or it is extremely volatile, turning such methods problematic. To address this issue, we develop self-adaptive techniques to select the outsourced content in a CDN infrastructure, which requires no apriori knowledge of request statistics. We identify clusters of “correlated” Web pages in a site, called Web site communities, and make these communities the basic outsourcing unit. Through a detailed simulation environment, using both real and synthetic data, we show that the proposed techniques are very robust and effective in reducing the user-perceived latency, performing very close to an unfeasible, off-line policy, which has full knowledge of the content popularity.     

Making Workflow Change Acceptable

Virtual professional communities are supported by network information systems composed from standard Internet tools. To satisfy the interests of all community members, a user-driven approach to requirements engineering is proposed that produces not only meaningful but also acceptable specifications. This approach is especially suited for workflow systems that support partially structured, evolving work processes. To ensure the acceptability, social norms must guide the specification process. The RENISYS specification method is introduced, which facilitates this process using composition norms as formal representations of social norms. Conceptual graph theory is used to represent four categories of knowledge definitions: type definitions, state definitions, action norms and composition norms. It is shown how the composition norms guide the legitimate user-driven specification process by analysing a case on the development of an electronic law journal.     

Cynipid gall-wasp communities correlate with oak chemistry

Host-plant association data, gathered from field surveys conducted throughout Florida and from the literature, were used to identify the specificity of cynipid gall inducers to one or more of six Quercus species that occur at Archbold Biological Station, Lake Placid, Florida, USA, including the red oaks Q. laevis, Q. myrtifolia, and Q. inopina, and the white oaks Q. chapmanii, Q. geminata, and Q. minima. Quercus myrtifolia had the highest cynipid richness and diversity (37 cynipid species, Shannon H = 3.61, Simpson's D = 0.97), followed by Q. chapmanii, Q. laevis, Q. inopina, Q. geminata, and finally Q. minima (10 species, H = 2.30, D = 0.90). All cynipid species showed strong fidelity to a particular host plant or a restricted set of host plants. An ordination of gall-wasp host associations indicated that the cynipid communities of each oak species were distinct and specific to a given oak species. Leaf samples taken from each oak species were analyzed for condensed and hydrolyzable tannins, total phenolics, lignin, cellulose and hemicellulose, nitrogen, and carbon. All of these chemical traits, with the exception of carbon, differed by oak species, and the differences were strongly correlated with the axes of the cynipid--species ordination. These results suggest that gall-wasp occurrence is influenced by oak chemistry and imply that experimental studies of cynipid gall inducers that examine host-plant chemistry and female oviposition choice and larval performance will yield useful insights.     

采油微生物发酵液中各组分对原油量测定的影响

原油量的测定是原油及其产品分析的必要环节,根据芳香烃在255nm具有特征吸收峰,可以通过测定芳香烃量间接表征原油量。本研究采用紫外分光光度法测定原油量,分析采油微生物发酵液中各组分、pH和采油微生物细胞内含物对原油量测定的影响。结果表明,上述因素对该方法测定原油量均没有显著影响,采用紫外分光光度法可以快速、准确地测定微生物采油过程中的原油量。