Immobilization of Lathyrus cicera Amine Oxidase on Magnetic Microparticles for Biocatalytic Applications

Amine oxidases are enzymes belonging to the class of oxidoreductases that are widespread, from bacteria to humans. The amine oxidase from Lathyrus cicera has recently appeared in the landscape of biocatalysis, showing good potential in the green synthesis of aldehydes. This enzyme catalyzes the oxidative deamination of a wide range of primary amines into the corresponding aldehydes but its use as a biocatalyst is challenging due to the possible inactivation that might occur at high product concentrations. Here, we show that the enzyme’s performance can be greatly improved by immobilization on solid supports. The best results are achieved using amino-functionalized magnetic microparticles: the immobilized enzyme retains its activity, greatly improves its thermostability (4 h at 75 °C), and can be recycled up to 8 times with a set of aromatic ethylamines. After the last reaction cycle, the overall conversion is about 90% for all tested substrates, with an aldehyde production ranging between 100 and 270 mg depending on the substrate used. As a proof concept, one of the aldehydes thus produced was successfully used for the biomimetic synthesis of a non-natural benzylisoquinoline alkaloid.     

聚乙烯吡咯烷酮对酸诱导液相沉积体系中GeO_2颗粒形貌的影响

在室温下,通过酸诱导液相沉积的方法,制备出了二氧化锗(GeO2)颗粒。制得的GeO2颗粒通过扫描电子显微镜(SEM),透射电子显微镜(TEM)和X射线衍射(XRD)进行表征。实验结果表明,向前驱液中加入酸,可以诱导锗酸根离子转化成立方体型的GeO2颗粒析出。而向反应液中再加入少量的聚乙烯吡咯烷酮(PVP)后,会析出纺锤体型的GeO2晶体颗粒。     

基于复合材料理论的土壤——根系力学研究

从微观的角度研究了根系——土壤复合体的宏观力学性质,并通过计算机模拟试验,研究了土壤和根系——土壤复合体的应变。结果表明,生态防护对加固土壤、保护边坡体有可支持的力学依据,能够有效的提高土壤的力学性质。     

The Use of Distinctive Monoclonal Antibodies in FMD VLP- and P1-Based Blocking ELISA for the Seromonitoring of Vaccinated Swine

The serum neutralization (SN) test has been regarded as the “gold standard” for seroconversion following foot-and-mouth disease virus (FMDV) vaccination, although a high-level biosafety laboratory is necessary. ELISA is one alternative, and its format is constantly being improved. For instance, standard polyclonal antisera have been replaced by monoclonal antibodies (MAbs) for catching and detecting antibodies, and inactive viruses have been replaced by virus-like particles (VLPs). To the best of current knowledge, however, no researchers have evaluated the performances of different MAbs as tracers. In previous studies, we successfully identified site 1 and site 2 MAbs Q10E and P11A. In this study, following the established screening platform, the VLPs of putative escape mutants from sites 1 to 5 were expressed and used to demonstrate that S11B is a site 3 MAb. Additionally, the vulnerability of VLPs prompted us to assess another diagnostic antigen: unprocessed polyprotein P1. Therefore, we established and evaluated the performance of blocking ELISA (bELISA) systems based on VLPs and P1, pairing them with Q10E, P11A, S11B, and the non-neutralizing TSG MAb as tracers. The results indicated that the VLP paired with S11B demonstrated the highest correlation with the SN titers (R² = 0.8071, n = 63). Excluding weakly positive serum samples (SN = 16–32, n = 14), the sensitivity and specificity were 95.65% and 96.15% (kappa = 0.92), respectively. Additionally, the P1 pairing with Q10E also demonstrated a high correlation (R² = 0.768). We also discovered that these four antibodies had steric effects on one another to varying degrees, despite recognizing distinct antigenic sites. This finding indicated that MAbs as tracers could not accurately detect specific antibodies, possibly because MAbs are bulky compared to a protomeric unit. However, our results still provide convincing support for the application of two pairs of bELISA systems: VLP:S11B-HRP and P1:Q10E-HRP.     

复合电耙道浅孔留矿采矿法试验研究

针对老柞山金矿矿体形态复杂,下盘矿体膨胀的开采技术条件,提出并试验了复合电耙道浅孔留矿采矿法．获得了显著的经济效益和社会效益,对类似矿山具有推广应用价值。     

相变换热器应用于电站锅炉的探讨

阐述了复合相变换热器是一种新型的换热器,可以控制低温受热面金属不发生低温腐蚀,降低烟气的排放温度,该技术可用于各种燃煤、燃油、燃气锅炉、窑炉、注气炉、加热炉等换热设备,目前复合相变换热器应用于工业锅炉和循环流化床已有不少成功案例[1-3],而应用于大型电站锅炉还很少有报道,结合某330 MW电站锅炉尾部加装复合相变换热器的运行情况,对复合相变换热器应用于电站锅炉的运行情况及优缺点做了简要探讨。     

超声电机定子的简化复合梁模型裂纹扩展分析与实验

超声电机工作环境非常复杂,压电陶瓷极易发生断裂失效。为了分析研究电场、预压力及裂纹位置对压电陶瓷断裂失效的影响,将超声电机的定子沿周向简化为弹性复合梁模型,并在压电陶瓷上预制贯穿裂纹。首先用Ansys有限元软件对简化模型进行仿真,分析得到增大电场、预压力能增加裂纹尖端应力值,裂纹更加容易扩展。然后通过实验验证得到增大电场强度裂纹更加容易扩展。     

熔融碳酸盐燃料电池(MCFC)用γ-LiAlO_2粉末的制备

本文简介了熔融碳酸盐燃料电池(MCFC)及其优点;叙述了合成LiAlO_2的方法,用Al_2O_3(a,γ)成功地制得了稳定的适合于MCFC用的γ-LiAlO_2,在MCFC工作温度(923K)下,β-LiAlO_2是不稳定的;973-1073K时,β-LiAlO_2向γ-LiAlO_2转变速度明显增大;a-LiAlO_2要在1073K以上转变为γ-LiAlO_2.     

8605运输巷复合顶板巷道稳定性控制研究

为探究高应力情况下复合顶板的治理控制方式,以煤峪口矿8605工作面复合顶板巷道为工程背景,研究了高应力复合顶板的变形破坏机理,并确定采用“帮顶同治”的围岩控制策略.现场应用实践表明,8605工作面运输巷表面位移变化稳定时间长,锚杆索受力平稳,巷道完整性控制效果好,能够满足矿井生产技术需要.