长岛海缆和风电场对末端电压的影响与解决方案

风电场实际发电量受来风的影响从零至最大发电量之间变化,使风电场与主网连接点的电压变动率很大。长岛风电场通过110kV海缆与大陆主电网连接,由于电缆的电容电感分布参数加剧了电压的变化。当主网往风电场送电并且输送的无功较小时需要补偿感性无功以补充电缆的充电电容,而风电场往主网送电时在风电场处需补偿容性无功。     

基于需求响应的虚拟电厂多时间尺度优化调度

随着我国不断加快新型电力系统建设,风电、光伏等越来越多的分布式资源大规模并网,但其随机性、波动性及分散性的特点给电力系统带来了多重不确定性。提出了一种考虑价格型需求响应的虚拟电厂多时间尺度优化调度方法,在日内调度模型中引入用户价格型需求响应,对用户负荷进行精准调控;采用基于蒙特卡罗与曼哈顿概率距离的场景生成与削减法处理风光出力、电价不确定性,减少日前日内出力偏差,再结合日前调度构成了多时间尺度优化调度模型,并采用滚动优化得到日内优化调度结果。以湖南某小型虚拟电厂系统进行算例仿真,结果表明,所提多时间尺度优化调度策略能够精确预测风光出力及用户负荷,在有效抑制功率波动的同时保证了系统的经济性。     

The Impact of Plant Phytochemicals on the Gut Microbiota of Humans for a Balanced Life

The gastrointestinal tract of humans is a complex microbial ecosystem known as gut microbiota. The microbiota is involved in several critical physiological processes such as digestion, absorption, and related physiological functions and plays a crucial role in determining the host’s health. The habitual consumption of specific dietary components can impact beyond their nutritional benefits, altering gut microbiota diversity and function and could manipulate health. Phytochemicals are non-nutrient biologically active plant components that can modify the composition of gut microflora through selective stimulation of proliferation or inhibition of certain microbial communities in the intestine. Plants secrete these components, and they accumulate in the cell wall and cell sap compartments (body) for their development and survival. These compounds have low bioavailability and long time-retention in the intestine due to their poor absorption, resulting in beneficial impacts on gut microbiota population. Feeding diets containing phytochemicals to humans and animals may offer a path to improve the gut microbiome resulting in improved performance and/or health and wellbeing. This review discusses the effects of phytochemicals on the modulation of the gut microbiota environment and the resultant benefits to humans; however, the effect of phytochemicals on the gut microbiota of animals is also covered, in brief.     

沈阳机床技改项目弱电智能化设计

沈阳机床（集团）有限责任公司的弱电智能化设计成功地将智能卡应用系统与MES有效的集成,为国内首创。生产物流条码化管理使其物流管理水平走在了国内机械行业的前列。系统采用了优化的制冷机群控、冷却塔群控技术,并在保持冷冻水大温差的条件下采用了变冷冻水温度的节能环保设计。全网冗余设计、弹性架构并融合安全的计算机网络充分保证厂区网络高可靠性、可扩展性和安全性。设计充分体现了信息化融合工业化的数字化工厂理念。     

Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis

Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type IIS restriction recognition sites. In the case of cloning CRISPR constructs by using golden gate (GG) cloning, this narrows down the scope of guide RNA (gRNA) picks because the selection of a good gRNA for successful genome editing requires some obligation of fulfillment, and it is unwanted if a good gRNA candidate cannot be picked only because it has an internal type IIS restriction recognition site. In this article, we have shown that the presence of a type IIS restriction recognition site in a gRNA does not affect cloning and subsequent genome editing. After each step of GG reactions, correct insertions of gRNAs were verified by colony color and restriction digestion and were further confirmed by sequencing. Finally, the final vector containing a Cas12a nuclease and four gRNAs was used for Agrobacterium-mediated citrus cell transformation. Sequencing of PCR amplicons flanking gRNA-2 showed a substitution (C to T) mutation in transgenic plants. The knowledge derived from this study could widen the scope of GG cloning, particularly of gRNAs selection for GG-mediated cloning into CRISPR vectors.