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31.
A. KarabchevskyAuthor Vitae S. KarabchevskyAuthor VitaeI. AbdulhalimAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(1):361-365
Surface plasmon resonance (SPR) imaging technique is proposed in which a diverging laser beam at given frequency was used to illuminate the entire sensor surface in Kretschmann-Raether configuration. A pattern of dark intensity line on bright background is observed corresponding to the SPR dip at an angular range depending on the refractive index of the adjacent analyte and monitored by a two-dimensional CCD detector. A novel Radon transform based detection algorithm for the SPR line pattern is proposed, which is non sensitive to the laser speckle noise and improves the accuracy. 相似文献
32.
Flavien PilletAuthor Vitae Carmen RomeraAuthor Vitae Emmanuelle TrévisiolAuthor Vitae Sophie BellonAuthor VitaeMarie-Paule Teulade-FichouAuthor Vitae Jean-Marie FrançoisAuthor Vitae Geneviève PratvielAuthor Vitae Véronique Anton LeberreAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(1):304-309
Surface plasmon resonance imaging (SPRi) is a label free technology for biomolecular interaction, which gives access to binding kinetic parameters from real time acquisition. It offers the possibility to test in a single run a large number of interactions, allowing rapid identification of the most suitable compounds toward a given biological entity. Until now, this technique has proven to be relevant for interaction between relatively large molecules (protein, antibodies, DNA) but has not been challenged yet for the screening of small molecules that can be of interested in the field of drug discovery. As a proof a principle, we have used SPRi to screen for interaction of several small molecules, referred to as G4-ligands on G-quadruplex DNA. This technology allowed to easy discrimination of the binding properties of four G4-ligands on quadruplex DNA models. 相似文献
33.
Xiangyi FangAuthor Vitae Chunlong LiuAuthor VitaeXiaoli ChengAuthor Vitae Yaoling WangAuthor VitaeYucong YangAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(2):760-764
A new type of array immunosensor was developed by combining surface plasmon resonance (SPR) and spectral imaging techniques. The system consisted of a monochromator as the wavelength scanning light source, a polarizer, Kretschmann-Raaether attenuated total reflection (ATR) configuration including array sensor chip, and a CCD camera. The images of transmitting light from ATR were recorded versus the wavelength. By averaging gray scales of the pixels in the area of every gold spot from the image series, the complete spectral resonance curve of all sensing spots on the array can be extracted in parallel. The performance of the developed system was evaluated by analyzing interactions of the anti-CD33 monoclonal antibody to its target leukemic cells using 11 cases of human bone marrow specimens. The specimens were also analyzed with flow cytometry method (FCM) for comparison. The initial results measured by the immunosensor array were corresponded with that of FCM, indicating that the developed parallel method might be clinically suitable for immunophenotyping of acute leukemias. The new sensor array system showed the merits of high-throughput, high sensitivity, high specificity, label free and operation convenient. Spots numbers of the array could be increased if suitable technology were adopted for manipulating the micro bio-liquids on the sensor array chip. 相似文献
34.
S. BaumgartenAuthor VitaeR. RobelekAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(2):798-804
Cells regulate their volume in response to changes in osmolarity of both, their extracellular and intracellular environments. As stability of the cell volume is a compelling exigency for cellular integrity, techniques for a sensitive, time-resolved volume measurement of adherently grown mammalian cells attract considerable interest, especially in the field of cell physiology and biology. In this study we apply a surface plasmon resonance (SPR) based biosensor for the comparative analysis of the volume responses of two renal epithelial cell types to non-isotonic challenges. The on-line, label-free and non-invasive biosensor format shows distinct similarities and differences in the reaction kinetics of the two cell types. Furthermore regulatory volume responses to the osmotic stimuli as well as their inhibition by Gd3+ ions can be observed with a high time-resolution. Limit-of-detection measurements indicate the high sensitivity of the sensor capable of detecting cellular volume responses of adherently grown mammalian cells to osmotic stimuli well below a bioanalytical relevant value of 5 mOsm/kg. 相似文献
35.
36.
Detection of sulphamethazine with an optical biosensor and anti-idiotypic antibodies 总被引:3,自引:0,他引:3
We describe the development of an assay for the detection of sulphamethazine in animal urine with a surface plasmon resonance (SPR) biosensor. In order to obtain a general assay that can easily be transferred to other veterinary drug residues, a monoclonal antibody against sulphamethazine and a corresponding anti-idiotypic antibody were used. The assay had a lower detection limit of 5 μg/l which is well below the maximum residue limits (MRL) of 100 μg/l and can be used for screening purposes in animal urine. The binding reaction between the antibodies as occurring during sensor application was characterized with respect to avidity and kinetic properties. 相似文献
37.
Koyo WatanabeAuthor Vitae 《Sensors and actuators. A, Physical》2012,173(1):36-40
A sensitivity optimization method for the detection of an intermediate layer using a surface plasmon sensor is presented. The dependence of detection sensitivity on distance from the metal surface and the average refractive index over the sensing region are considered. Based on the calculated results, the sensitivity for detecting an intermediate layer in a multilayered sample is determined by varying the refractive index and thickness of each layer. It is shown that, in particular cases, controlling the refractive index and thickness increases the detection sensitivity. The proposed method is useful for designing multilayered samples. 相似文献
38.
Seogil Oh Jungwoo Moon Taewook Kang Surin Hong Jongheop Yi 《Journal of Electroceramics》2006,17(2-4):999-1003
The use of surface plasmon resonance (SPR) spectroscopy has been applied to a wide variety of fields such as biosensors and
surface analysis instruments. In general, a SPR substrate is prepared using self-assembled monolayer (SAM) method of organic
molecules as receptor for the target on a layer of gold or silver. However, mesoporous inorganic materials such as SBA-15
have benefits as sensor substrate for SPR. Mesoporous silica has a large surface area which receptor molecule can be attached
and has a rigid body which has an excellent stability in the extreme condition compared to organic sensing layer. We prepared
an organic modified mesoporous silica and successfully immobilized it on a gold surface, in an attempt to use as a substrate
for SPR spectroscopy. For the comparison of sensitivity of prepared substrate, Pt2+ detection was selected as a model system. Substrate prepared in this study exhibited the capability of selective sensing
for Pt2+ ions. 相似文献
39.
Plasmon (electrostatic) resonances are treated as an eigenvalue problem for specific boundary integral equation. This leads to direct calculation of resonance values of permittivity and resonance frequency. The technique is illustrated by numerical examples of calculation of resonance frequencies for 3D nanoparticles. 相似文献
40.
Surface plasmon resonance sensor based on a single-mode polarization-maintaining optical fiber 总被引:5,自引:0,他引:5
A novel wavelength modulation-based fiber-optic surface plasmon resonance (SPR) sensor is reported which utilizes both polarization separation and broad band radiation depolarization in polarization-maintaining fibers to enhance sensor stability. Theoretical analysis of the sensing structure with ideally separated polarizations based on the mode of expansion and propagation method is presented. The effect of polarization cross-coupling was also analyzed in the approximation of an equivalent bulk optic structure. A laboratory prototype of the fiber-optic SPR sensor was characterized in terms of sensitivity and resolution. Experimental results indicate that this fiber-optic SPR sensor is able to resolve refractive index changes as low as 4×10−6 under moderate fiber deformations. 相似文献