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Siming LiuAuthor VitaeRuo YuanAuthor Vitae Yaqin ChaiAuthor VitaeHuilan SuAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(1):388-394
In this work, a novel label-free amperometric immunosensor has been constructed for detecting α-1-fetoprotein (AFP) based on nanocomposite of horseradish peroxidase (HRP) labeled carbon nanotubes (CNTs). First, the gold nanoparticles (AuNPs) were electrodeposited on the surface of the glass carbon electrode by electrochemical reduction of gold chloride tetrahydrate (HAuCl4) to immobilize horseradish peroxidase labeled carbon nanotubes (HRP-CNTs). Then HRP-CNTs bioconjugate was immobilized on the surface of the electrodeposited AuNPs layer by the combination of forces (coordination and electrostatic force). Subsequently, it was immersed into gold colloidal nanoparticles (GNPs) solution, which was used to immobilize antibody biomolecules (anti-AFP). Enhanced sensitivity was obtained by using bioconjugates featuring HRP labeled (HRP-CNTs), which had lager specific surface area and good electronic catalysis (current response signal) compared to carbon nanotubes. Under optimized conditions, the linear ranges were from 0.2 to 200 ng mL−1 with a detection limit of 0.067 ng mL−1 (at an S/N of 3). The proposed immunosenor showed good precision, acceptable stability and reproducibility and could be used for the detection AFP in normal human serum, which provided a potential alternative tool for the detection of protein in clinical diagnosis. 相似文献
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基于NTP的高精度时钟同步系统实现 总被引:10,自引:1,他引:9
Windows操作系统内置的NTP授时精度不高,分辨率最高只有10ms。给出一个基于Windows操作系统的计算机网络同步时钟实现方案,该方案可以有效提高计算机时钟同步精度,在LAN中时钟同步精度达250μs。同时采用了校正时钟频率误差算法,校正后的时钟长期计时误差能达到10天少于1s。 相似文献
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The conventional game theory is concerned with how rational individuals make decisions when they are faced with known payoffs. In the real world, sometimes the payoffs are not known and have to be estimated, and sometimes the payoffs are only approximately known. This paper develops a solution method for the two-person zero-sum game where the payoffs are imprecise and are represented by interval data. Since the payoffs are imprecise, the value of the game should be imprecise as well. A pair of two-level mathematical programs is formulated to obtain the upper bound and lower bound of the value of the game. Based on the duality theorem and by applying a variable substitution technique, the pair of two-level mathematical programs is transformed to a pair of ordinary one-level linear programs. Solving the pair of linear programs produces the interval of the value of the game. It is shown that the two players in the game have the same upper bound and lower bound for the value of the imprecise game. An example illustrates the whole idea and sheds some light on imprecise game. 相似文献
998.
A property-based attestation protocol for TCM 总被引:1,自引:0,他引:1
This paper presents a property attestation protocol for the security chip TCM (trusted cryptographic module) via analyzing the problems of the current property attestation, which is built on the property attestation model with the online trust third party. In the protocol the prover utilizes the zero-knowledge proof by the attribute certificates, configuration commitment and TCM signature, and attests its configuration and status which are compliant with the declarative security property. The protocol is ch... 相似文献
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