Application of functionalised carbon nanotubes immobilised into chitosan films in amperometric enzyme biosensors

A new approach for building a bio-conductive interface for enzyme immobilisation is described. This strategy permits very simple preparation of the enzyme biosensor and also reveals direct electron transfer features. A graphite-epoxy resin composite (GrEC) electrode modified with functionalised multi-wall carbon nanotubes (MWCNTs) immobilised by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide together with N-hydroxysuccinimide (EDC–NHS) in a chitosan (Chit) matrix was prepared and characterised by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of hexaammineruthenium (III) chloride. It was then used as a base for glucose oxidase (GOx) immobilisation by the simple method of crosslinking with glutaraldehyde (GA) with bovine serum albumin (BSA) as carrier protein. The resulting mediator-free biosensor was applied to the determination of glucose in amperometric mode at different applied potentials and the mechanism of reaction was also investigated by cyclic voltammetry, with and without dissolved oxygen in solution. Analytical parameters, as well as reproducibility, repeatability and stability were determined. Interferences were assessed using different compounds usually present in natural samples, such as wines, juices or blood, in order to evaluate the selectivity of the developed biosensor. The novel combination of carbon nanotubes immobilised with chitosan crosslinked with EDC–NHS and glucose oxidase immobilised by crosslinking with glutaraldehyde offers an excellent, easy to make biosensor for glucose determination without interferences.     

基于事件驱动的动态免疫分簇路由算法*

为了降低应用于突发事件监测的无线传感器网络的能量消耗,设计实现了一种基于事件驱动的动态免疫分簇路由算法。将生物免疫系统的工作机制应用到无线传感器网络事件驱动的动态分簇算法中,事件作为抗原,传感器节点作为抗体,抗体对抗原有记忆保存的功能,使得相似的抗原再次出现时对事件及时响应。相似事件再次发生且传感器节点符合能量要求时,可以直接调用抗体中的记忆,对事件进行快速建簇,节省了簇的建立过程所消耗的大量能量,增加了网络的数据传输量,延长了网络的生命周期。仿真结果表明,生物免疫机制的学习记忆特性可以有效提高事件驱动的动态分簇算法的网络性能。     

一种基于组策略的过滤式特征选择算法

MRMR算法具有快速、高效等优势,在处理高维数据方面较为流行。基于此,提出一种基于组策略的MRMR改进算法（MRMRE）,该算法不仅考虑单个特征属性的相关性与冗余性,同时针对特征组间的相互关系进行研究。算法以MRMR算法为框架,以CCA作为度量基准,选择SVMs作为基分类器,使其特征选择效果提升。在UCI机器学习数据库中图像与基因序列数据集上的大量实验表明：与MRMR算法相比,所提出的算法其特征选择结果具有更高的结果稳定性与分类精度。     

Multi‐Unit Bayesian Auction with Demand or Budget Constraints

We consider the problem of revenue maximization on multi‐unit auctions where items are distinguished by their relative values; any pair of items has the same ratio of values to all buyers. As is common in the study of revenue maximizing problems, we assume that buyers' valuations are drawn from public known distributions and they have additive valuations for multiple items. Our problem is well motivated by sponsored search auctions, which made money for Google and Yahoo! in practice. In this auction, each advertiser bids an amount b_i to compete for ad slots on a web page. The value of each ad slot corresponds to its click‐through‐rate, and each buyer has her own per‐click valuations, which is her private information. Obviously, a strategic bidder may bid an amount that is different with her true valuation to improve her utility. Our goal is to design truthful mechanisms avoiding this misreporting. We develop the optimal (with maximum revenue) truthful auction for a relaxed demand model (where each buyer i wants at most d_i items) and a sharp demand model (where buyer i wants exactly d_i items). We also find an auction that always guarantees at least half of the revenue of the optimal auction when the buyers are budget constrained. Moreover, all of the auctions we design can be computed efficiently, that is, in polynomial time.     

不锈钢活性炭滤器的氯腐蚀与防腐

本文就活性炭在预处理中脱氯的不锈钢滤器的氯腐蚀机理进行了探讨，并提出了不锈钢活性炭脱氯滤器的防腐方法和措施。     

石墨粒度对低炭镁炭砖性能的影响

以电熔镁砂和4种粒度(≤0.149mm、 ≤0.074mm、≤0.044mm和≤0.01mm)的天然鳞片 石墨为主原料,在石墨含量为4%的条件下,研究了 石墨粒度对低炭镁炭砖的物理性能、抗氧化性和热震 稳定性的影响。结果表明:用细石墨取代较粗石墨 (粒度≤0.149mm)制成的镁炭砖,其物理性能、抗氧 化性和热震稳定性都有了明显的改善,且都以加入粒 度≤0.074mm石墨的效果最好。其原因是细石墨的 加入改善了镁炭砖的基质结构。石　墨     

建设项目设计阶段投资控制系统的建立与分析

针对建设设计阶段独立使用价值工程、优化设计和限额设计等方法进行投资控制的局限性,借鉴系统论、控制论、信息论等思想和方法,建立了设计阶段投资控制系统。系统主要由探测元件、控制元件和执行元件组成,探测元件进行设计阶段的信息收集;控制元件通过审核将各项投资控制活动与投资控制目标进行对比,提出应对策略;执行元件完成应对策略。通过投资控制系统的执行,实现对设计阶段各项活动的全面系统控制,从管理层面上解决设计阶段投资控制活动割裂问题,进而改善设计阶段整体投资控制效果。     

A method for observing the three-dimensional morphologies of inclusions in steel

A method for observing the three-dimensional morphologies of inclusions by deeply eroding a steel sample with a kind of organic solution composed of bromine water,acetone,and HC1 (volume ratio,45∶45∶10...     

关于文博馆品牌建设的思考——以南通纺织职业技术学院文博馆为例

作为大学文化建设的重要窗口,南通纺织职业技术学院文博馆肩负着文化传承、发展与创新的重大责任。为充分彰显其文化教育功能和文化品牌效应,应进一步加强内涵与外延建设,以科研为动力,积极探索文博馆的运行机制。     

An ergonomic comparison of firearm safety mechanisms

As with most machines, the integral safety mechanism on firearms is vital to injury or fatality free operation. Presently, there is little or no standardisation in the design of these mechanisms. In this investigation, five existing designs found on both military and commercial rifles were evaluated ergonomically to determine the most effective characteristics for incorporation into future designs. The designs were evaluated experimentally on ease of use, visual effectiveness and operational impact. Three groups, representing a total of 30 subjects with widely varying experience, were selected. Results strongly suggest that safeties whose actuators are mounted within easy reach of the trigger finger are preferred and have the least operational impact. Subjects also preferred and were more adept at recognising safety status when the indicator was located on the receiver/barrel along the normal line of sight. Subjects more often correctly identified safety status when the indicator utilised colouring, was clearly marked and/or was in the normal line of sight. The results of this research prove that ergonomics can contribute to the understanding of firearm safety dynamics. The two essential components of safety mechanism design identified in this investigation, unambiguous status visibility and impact-free operation, are also likely to have implications in non-firearm safety mechanism design. This is particularly true for devices whose inadvertent operation can cause injury, as well as systems in which operational effectiveness can be jeopardised when attentiveness or operational control are lost in the process of actuating a poorly designed safety mechanism.