Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B₁ (AFB₁) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond between the residues H44 and L100. The stability of scFv (H4) increased from a GdnHCl₅₀ of 2.4 M to 4.2 M after addition of the H44-L100 disulfide bond. Size exclusion chromatography revealed that the scFv (H44-L100) mutant existed primarily as a monomer, and no aggregates were detected. An affinity assay indicated that scFv (H4) and the scFv (H44-L100) mutant had similar IC₅₀ values and affinity to AFB₁. Our results indicate that interdomain disulfide bonds could stabilize scFv without affecting affinity.     

任意分布的均值的渐近有效估计

讨论一般随机分布的均值的区间估计问题,给出了一种求任意分布均值的置信区间的抽样方案：证明此抽样方案渐近有效,得出了关于任意分布均值的置信区间的新结果。     

一类分式规划的对偶定理

本文在预不变凸性条件下,利用有效解概念,结出一类分式线性规划的一些对偶定理,这些定理为求解这类分式线性规划提供了简捷方法。     

澳大利亚变电站在线状态监视系统

电力市场的竞争促使电力公司降低电网检修成本，减少停电的次数，尤其是减少因设备缺陷而造成的被迫停电频率和停电时间。为实现这一目标，有效的状态监视将是一个强有力的工具。文中介绍澳大利亚TransGrid公司采用在线状态监测技术的设计理念、应用中的问题及解决措施，最后示出应用效果。     

A WELL-CONDITIONED MATRIX FOR (kuX)X WITH DISCONTINUOUS k

The traditional tridiagonal matrix approximating the one-dimensional heat equation is ill-conditioned when heat conductivity changes radically. An algebraic reformulation of the tridiagonal produces a well-conditioned matrix. Additional variables are rates q=−ku_x at interfaces between radical changes in k. A reduced matrix amounts to a coarse approximation.     

考虑压敏效应的技术极限井距计算方法研究

传统技术极限井距计算方法未考虑特低渗透油藏压敏效应对注采井间驱替压力及启动压力梯度的影响,导致计算结果与矿场实际情况存在较大差距.针对这一实际问题,该研究通过建立考虑压敏效应油水井附近驱替压力公式及变启动压力梯度计算公式,基于传统技术极限井距计算公式,推导了考虑压敏效应的技术极限井距计算公式,并对模型进行参数分析.结果...     

A robust preconditioning technique for the extended finite element method

The extended finite element method enhances the approximation properties of the finite element space by using additional enrichment functions. But the resulting stiffness matrices can become ill‐conditioned. In that case iterative solvers need a large number of iterations to obtain an acceptable solution. In this paper a procedure is described to obtain stiffness matrices whose condition number is close to the one of the finite element matrices without any enrichments. A domain decomposition is employed and the algorithm is very well suited for parallel computations. The method was tested in numerical experiments to show its effectiveness. The experiments have been conducted for structures containing cracks and material interfaces. We show that the corresponding enrichments can result in arbitrarily ill‐conditioned matrices. The method proposed here, however, provides well‐conditioned matrices and can be applied to any sort of enrichment. The complexity of this approach and its relation to the domain decomposition is discussed. Computation times have been measured for a structure containing multiple cracks. For this structure the computation times could be decreased by a factor of 2. Copyright © 2010 John Wiley & Sons, Ltd.     

空间结构健康监测研究现状与展望

空间结构是大跨度、大空间和大面积建筑结构的主要形式,在国家基础设施与城市建设领域有广泛应用,其结构安全性至关重要。空间结构健康监测通过传感技术准确获取结构响应,实时反映结构的性能状态,为空间结构施工、运营与管理决策提供科学的依据与指导。经过多年的研究和工程实践,已经发展了适合大型空间结构的物联网无线传感监测技术,建立了多维数据分析理论,并在国家体育场、国家速滑馆、北京大兴国际机场航站楼等重大工程中实现规模化应用,取得了阶段性研究进展。通过对空间结构健康监测的研究及应用现状进行系统总结,疏理空间结构健康监测的特点,围绕荷载与响应多维传感及大面域传输网络综述了其传感监测技术的研究现状,归纳了结构荷载分析、响应分析以及结构状态评估的监测数据分析理论。同时,基于融合大数据、云计算以及人工智能展望其未来研究方向,旨在进一步推动空间结构健康监测的创新与发展。     

机翼极限环颤振的CFD/CSD全隐式紧耦合方法

针对轴承振动实时状态监控的需要,从符号动力学角度提出了符号聚合近似与Lempel-ziv复杂度（SAX-LZC）融合的振动监控参数。首先,以Logistic映射和Duffing方程为对象,从理论角度验证了SAX-LZC对动力学结构表征的准确性,并验证了该指标的抗噪能力和计算效率;其次,将SAX-LZC指标与信息熵、样本熵、多分段Lempel-ziv复杂度等动力学参数性能进行了综合对比;最后,从实验角度对轴承早期微弱异常进行了监测,并对轴承典型故障进行了特征提取。理论研究结果表明,SAX-LZC具有动力学结构表征准确、抗噪能力好、计算高效简洁等优点,克服了常规动力学参数工程应用能力弱的问题。实验研究结果表明,SAX-LZC对早期微弱异常有准确的监测,对不同种类故障具有较好的区分度,弥补了时域和频域对轴承微弱故障表征能力不足的缺陷,是一种轴承振动实时状态监控与故障特征提取的有效参数。