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1.
Isothermal nucleic-acid amplification methods such as Loop-Mediated isothermal AMPlification (LAMP) are increasingly appealing alternatives to PCR for use in portable diagnostic system due to the low cost, weight, and power requirements of the instrumentation. As such, interest in developing new probes and other functionality based on the LAMP reaction has been intense. Here, we report on the development of duplexed LAMP assays for pathogen detection using spectrally unique Assimilating Probes. As proof of principle, we used a reaction for Salmonella enterica as a model coupled with a reaction for λ-phage DNA as an internal control, as well as a duplexed assay to sub-type specific quarantine strains of the bacterial wilt pathogen Ralstonia solanacearum. Detection limits for bacterial DNA analyzed in individual reactions was less than 100 genomic equivalents in all cases, and increased by one to two orders of magnitude when reactions were coupled in duplexed formats. Even so, due to the more robust activity of newly available strand-displacing polymerases, the duplexed assays reported here were more powerful than analogous individual reactions reported only a few years ago, and represent a significant advance for incorporation of internal controls to validate assay results in the field. 相似文献
2.
We present a data-driven method for monitoring machine status in manufacturing processes. Audio and vibration data from precision machining are used for inference in two operating scenarios: (a) variable machine health states (anomaly detection); and (b) settings of machine operation (state estimation). Audio and vibration signals are first processed through Fast Fourier Transform and Principal Component Analysis to extract transformed and informative features. These features are then used in the training of classification and regression models for machine state monitoring. Specifically, three classifiers (K-nearest neighbors, convolutional neural networks and support vector machines) and two regressors (support vector regression and neural network regression) were explored, in terms of their accuracy in machine state prediction. It is shown that the audio and vibration signals are sufficiently rich in information about the machine that 100% state classification accuracy could be accomplished. Data fusion was also explored, showing overall superior accuracy of data-driven regression models. 相似文献
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基于子波变换的涡街流量传感器信号分析 总被引:6,自引:0,他引:6
长期以来 ,如何提取潜在噪声下的涡街流量信号一直是个问题。流体流速脉动 ,局部阻力 ,随机振动———所有这些因素都给解决这一问题带来难度。文章应用子波变换消噪理论 ,从软件滤波的角度分析了强噪声干扰下的涡街流量信号 ,并提出了单支重构计数方法。分析结果表明 ,这种方法对低流速流量计量效果很好 ,能够有效地扩展量程下限 相似文献
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介绍一种经济实用的用普通磁带记录与回放超低频信号的方法,该方法在地质,建筑工程和医疗等行业中有一定的实用价值。 相似文献
7.
Guangyan Li W. Ian Hamilton Ged Morrisroe Theresa Clarke 《Cognition, Technology & Work》2006,8(1):30-40
A study was carried out using simulation to investigate driver responses to lineside signals and signs at various approach
speeds. The objectives of the study were: (1) to find out whether train speed would significantly affect signal/sign reading;
(2) to examine at which point certain types of signs or signals could be detected or recognised, and (3) to determine a speed
cut-off level above which certain types of signs or signals are no longer recognisable or detectable. Fifty-seven train drivers
from 12 Train Operating Companies in the UK participated in the trials. Twenty different types of lineside signs and ten types
of signals were tested under six different approach speeds ranging from 100 to 350 km/h (62–218 mph). Driver performance measures
were ‘time remaining to the signal/sign’ at the point of detection or recognition, and reading error rate. The results showed
a significant influence of train speed on driver responses to lineside signals/signs and demonstrated a non-linear relationship
between driver responses to signals/signs and approach speed. This has been used to estimate a maximum approach speed limit
within which a specific signal or sign can be correctly detected or recognised. The findings and implications of the study
are discussed in the paper. 相似文献
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本文提出生种新的带限信号的外推方法,该方法能够很好地抑制噪声干扰,且计算复杂性较低,本文给出算法,证明了收敛性,最后给出了误差计算机实验结果。 相似文献
10.
A damage identification technique for CF/EP composite laminates using distributed piezoelectric transducers 总被引:8,自引:0,他引:8
In this study, a damage identification approach was developed for carbon fibre/epoxy composite laminates with localized internal delamination. Propagation of the Lamb wave in laminates and its interaction with the delamination were examined. The fundamental symmetric Lamb wave mode, S0, and the lowest order shear wave mode, S0′, were chosen to predict damage location. A real-time active diagnosis system was therefore established. This technique uses distributed piezoelectric transducers to generate and monitor the ultrasonic Lamb wave with narrowband frequency. The two-way switches were employed to minimize the number of transducers. A signal-processing scheme based on the time–frequency spectrographic analysis was utilised to extract useful diagnostic information. Also, an optimal identification method was applied on damage searching procedure to reduce errors and obtain the diagnostic results promptly. Experiments were conducted on [0/−45/45/90]s CF/EP laminates to verify this diagnosis system. The results obtained show that satisfactory detection accuracy could be achieved. 相似文献