Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor. 相似文献
1. 1. System design optimization and validation for single-speed heat pump by S.K. Fischer and C.K. Rice, Oak Ridge National Laboratories.
2. 2. Analysis of on/off cycling for an air-to-air heat pump operating in the heating mode by W.A. Miller, Oak Ridge National Laboratories.
3. 3. Field measured cycling, frosting and defrosting losses for a high efficiency air source heat pump by V.D. Baxter and J.C. Moyers, Oak Ridge National Laboratories.
4. 4. Design and available energy analysis of a heating-only residential heat pump for the Western Pacific Northwest by D.E. Elger, C.M. Reistad and S. Lang, Oregon State University.
5. 5. A study of heat pump service life by Nance C. Lovvorn, Alabama Power Company and Carl C. Hiller, Electric Power Research.
Résumé
1. 1. Optimisation de la conception des systèmes et application à la pompe à chaleur à une seule vitesse;
2. 2. analyse du cycle par tout ou rien d'une pompe à chaleur air-air fonctionnant en mode de chauffage;
3. 3. pertes en fonctionnement cyclique, par givrage et dégivrage mesurées sur place pour une pompe à chaleur de grand rendement dont la source est l'air;
4. 4. conception et analyse de l'énergie disponible d'une pompe à chaleur uniquement pour le chauffage de locaux résidentiels sur la côte du Pacifique nord-ouest occidental;
5. 5. étude de la durée de vie d'une pompe à chaleur.
Les noms et les addresses des auteurs se trouvent dans le sommaire anglais. 相似文献
The purpose of this study is to clarify damage process of “Type IV cracking” in weldment of a 2.25Cr-1Mo steel and to propose a micro damage prediction method. From continuous observation under a creep-fatigue test, it was found that spherical shape voids initiated and grew on grain boundaries in fine grain region and these voids grow continuously by changing their shape to crack-like. Both spherical and crack-like void growth rate equations were derived from the proposed void growth model. It was indicated that measured void growth rate under the creep-fatigue loading was well predicted by the growth rate equations. 相似文献