急性腹痛54例临床分析

目的增加对急性腹痛病因的认识,提高诊断率,探讨治疗方法。方法随机抽取以急性腹痛为主要表现的内科住院例54例进行病因、症状、体征、辅助检查及治疗的统计分析。结果以急性腹痛为主要临床表现的疾病病变部位,腹腔内器官占92.6%,腹腔外器官占7.4%,最常见的病因为急性胰腺炎27.8%。结论以急性腹痛为主要表现的疾病病因复杂,病情多变,应特别重视其诊断及治疗。     

西医结合中医治疗急性脑梗死临床研究

目的观察中西医结合治疗急性脑梗死的临床疗效。方法233例急性脑梗死患者随机分为两组,对照组116例采用中医辨证治疗,治疗组117例在此基础上结合西医治疗。采用神经功能缺损评分量表(CSS)进行疗效评价。结果以90d为试验终点时间。治疗组总有效率74.11%(83/112例),优于对照组的60.36%(67/111例)。经统计学处理差异均有显著性,治疗组优于对照组(P<0.05)。结论中医辨证结合西医治疗急性脑梗死有较为明显的疗效优势。     

急性闭合性颅脑损伤患者肠道菌群变化

目的观察急性闭合性颅脑损伤患者肠道细菌数量的变化。方法选取交通事故导致的12例急性闭合性颅脑损伤的男性患者的粪便进行细菌培养,观察细菌数量的变化。测定血浆去甲肾上腺素浓度。结果颅脑损伤组与对照组相比乳酸杆菌数量明显减少,大肠杆菌数量显著增多(P值均<0.01)。颅脑损伤后血浆去甲肾上腺素水平较对照组明显升高(P<0.01)。结论急性闭合性颅脑损伤可引起肠道菌群失调。     

氯消毒副产物及其影响因素的研究概况

氯消毒因其效果可靠、操作简便、价格便宜等优点而成为污水消毒中广泛采用的方法,但氯消毒过程中产生的有毒副产物可能带来的化学安全问题则成为越来越受关注的焦点.本文主要是研究氯消毒副产物的生成及其影响因素,为污水氯消毒实践中消毒条件的选择、水质控制及相关水质安全标准的制定提供依据.     

Cytotoxic activity to acute myeloid leukemia cells by Antp-TPR hybrid peptide targeting Hsp90

We previously reported that Antp-TPR hybrid peptide inhibited the interaction of Hsp90 with TPR2A and had selective cytotoxic activity discriminating between normal and cancer cells to induce cancer cell death. In this study, we investigated the cytotoxic activity of Antp-TPR peptide toward acute myeloid leukemia (AML) cells. It was demonstrated that Antp-TPR peptide induced AML cell death in cell lines such as U937, K562, THP-1, and HL-60 via activation of caspases 3 and 7, and disruption of mitochondrial membrane potential. Conversely, Antp-TPR peptide did not reduce the viability of normal cells including peripheral blood mononuclear cells (PBMCs), although both geldanamycin and 17-AAG, small-molecule inhibitors of Hsp90, mediated cytotoxicity to these normal cells at low concentrations. In addition, mutation analysis of TPR peptide demonstrated that the highly conserved amino acids Lys and Arg were critical to the cytotoxic activity. These results indicated that Antp-TPR hybrid peptide would provide potent and selective therapeutic options in the treatment of AML.     

三氧化二砷联合全反式维甲酸治疗初发急性早幼粒细胞白血病50例疗效观察

目的 观察三氧化二砷(ATO)联合全反式维甲酸(ATRA)治疗初发急性早幼粒细胞白血病(APL)的疗效.方法 98例初发APL患者分为对照组和治疗组,对照组48例,治疗组50例.对照组采用常规ATRA+DA双诱导方案治疗;治疗组采用ATRA每天25 mg/m2,ATO每天0.15 mg/kg(ATRA后第10天开始)联合治疗,直至完全缓解(CR),CR后接受ATO和ATRA联合巩固治疗.比较两组CR率、PML-RAR α融合基因转阴时间及5年无病生存率.结果 对照组和治疗组CR率分别为89.5%(43/48)和90.0%(45/50),获得CR时间分别为(30.0±5.1)d和(28.1±4.4)d,两组CR率(x2=-0.068,P=0.946)及获得CR时间(t=1.757,P=0.083)相比差异均无统计学意义.在所有获得CR的患者中,3例分别在CR后第276、385和394天复发.所有患者发病时PML-RAR α融合基因均阳性,对照组和治疗组CR时分别有25.0%(5/20)和29.4%(5/17)转阴,巩固后分别有92.5%(37/40)和97.6%(41/42)转阴.对照组和治疗组5年无病生存率分别为(85.3±5.9)%和(87.6±5.6)%,差异无统计学意义(x2=0.232,P=0.630).结论 ATO联合ATRA能有效治疗初发APL患者,可以作为常规化疗方案外的另一选择.     

Bleeding Disorders in Primary Fibrinolysis

Fibrinolysis is a complex enzymatic process aimed at dissolving blood clots to prevent vascular occlusions. The fibrinolytic system is composed of a number of cofactors that, by regulating fibrin degradation, maintain the hemostatic balance. A dysregulation of fibrinolysis is associated with various pathological processes that result, depending on the type of abnormality, in prothrombotic or hemorrhagic states. This narrative review is focused on the congenital and acquired disorders of primary fibrinolysis in both adults and children characterized by a hyperfibrinolytic state with a bleeding phenotype.     

Targeting Intra-Pulmonary P53-Dependent Long Non-Coding RNA Expression as a Therapeutic Intervention for Systemic Lupus Erythematosus-Associated Diffuse Alveolar Hemorrhage

Diffuse alveolar hemorrhage (DAH) in systemic lupus erythematosus (SLE) is associated with significant mortality, requiring a thorough understanding of its complex mechanisms to develop novel therapeutics for disease control. Activated p53-dependent apoptosis with dysregulated long non-coding RNA (lncRNA) expression is involved in the SLE pathogenesis and correlated with clinical activity. We examined the expression of apoptosis-related p53-dependent lncRNA, including H19, HOTAIR and lincRNA-p21 in SLE-associated DAH patients. Increased lincRNA-p21 levels were detected in circulating mononuclear cells, mainly in CD4+ and CD14+ cells. Higher expression of p53, lincRNA-p21 and cell apoptosis was identified in lung tissues. Lentivirus-based short hairpin RNA (shRNA)-transduced stable transfectants were created for examining the targeting efficacy in lncRNA. Under pristane stimulation, alveolar epithelial cells had increased p53, lincRNA-p21 and downstream Bax levels with elevated apoptotic ratios. After pristane injection, C57/BL6 mice developed DAH with increased pulmonary expression of p53, lincRNA-p21 and cell apoptosis. Intra-pulmonary delivery of shRNA targeting lincRNA-p21 reduced hemorrhage frequencies and improved anemia status through decreasing Bax expression and cell apoptosis. Our findings demonstrate increased p53-dependent lncRNA expression with accelerated cell apoptosis in the lungs of SLE-associated DAH patients, and show the therapeutic potential of targeting intra-pulmonary lncRNA expression in a pristane-induced model of DAH.     

91例剖宫产产后出血临床分析

目的探讨剖宫产产后出血的原因及临床特点。方法以我院2007年1月至2009年12月91例剖宫产产后出血患者为研究组,以我院同期行剖宫产未发生出血者2157例为对照组。比较分析2组患者产科情况,总结分析研究组产后出血量、出血原因和治疗方法。结果本组剖宫产产后出血91例,占同期剖宫产总数的4.05%,经积极治疗,无一例死亡;研究组平均年龄、多胎妊娠及伴有合并症比例显著高于对照组(P<0.01);剖宫产产后出血原因主要有宫缩乏力、胎盘因素及手术因素3类,其构成比分别为60.33%、24.18%和5.49%;结论剖宫产产后出血主要原因复杂,临床上应早期预见并消除产后出血的危险因素,术中加强规范操作,科学合理的处置产后出血,以减少死亡率。     

序贯性通气治疗重症急性左心衰竭伴呼吸衰竭的临床观察

目的观察有创-无创序贯通气对重症急性左心衰伴呼吸衰竭的治疗效果。方法对24例常规治疗无效的重度急性左心衰伴呼吸衰竭患者加用序贯通气治疗,比较治疗前后的临床症状、生命体征及血气分析结果。结果 24例患者20例经序贯通气后低氧血症较前明显好转,生命体征及血气分析指标也有明显改善(P<0.05)。结论序贯通气能及时有效地缓解急性左心衰竭所致的低氧血症和高碳酸血症,是抢救重症急性左心衰发作的有效措施,值得临床推广。