PSE-HPLC 法测定食品中叔丁基羟基茴香醚和2,6- 二叔丁基羟基甲苯

目的：建立测定食品中抗氧化剂叔丁基羟基茴香醚(BHA)和2,6- 二叔丁基羟基甲苯(BHT)加压溶剂萃取-高效液相色谱法(PSE-HPLC 法)。方法：采用正交试验对影响PSE 萃取效率的温度、压力、萃取溶剂、萃取时间进行优化,联合HPLC 进行测定,并确定分别以BHA 提取量、BHT 提取量以及总量为评价指标的最优条件。结果：PSE-HPLC 法测定BHT 和BHA 的相对标准偏差(RSD)为0.5%～3.1%,并且在1.0～200.0μg/mL 范围内色谱峰面积与组分质量浓度均有很好的线性相关性(r ≥ 0.9997),检出限为0.05μg/mL,在最优条件下的回收率为92.60%～97.80%。结论：PSE-HPLC 法简便、快速、效率高,方法的重现性、线性相关性以及检出限理想,适用于食品中BHT 和BHA 含量及两者总量的同时快速检测。     

The effect of rubber closures on the haze state of ceftriaxone sodium for injection

The migration of volatile components into the headspace of glass vials from rubber closures is a potential source of haze formation in reconstituted solutions of powders for parenteral administration. In this paper, a headspace method was used to extract volatile substances from different types of rubber closures used in ceftriaxone sodium for injection whose clarity were poor. The extracts obtained were analyzed by gas chromatography (GC)-mass spectrometry (MS). An antioxidant in rubber closures, butylated hydroxytoluene (BHT), was found to affect the haze state of ceftriaxone sodium for injection.     

塑胶跑道抗老剂BHT在非固体原料中含量与成品中释放量关系探究

建立了气相色谱-质谱法测定塑胶跑道非固体原料中2,6-二叔丁基对甲苯酚(BHT)含量的测试方法,在(1～100)mg/L范围内,方法线性相关系数R²=0.99795,检出限为0.012g/kg,定量限为0.040g/kg,RSD在(1.29～2.56)%。将含有BHT的非固体原料制作成塑胶跑道成品,用GB 36246-2018中总挥发性有机化合物(TVOC)释放量测试方法测试BHT释放量。建立非固体原料中BHT含量与成品中BHT释放量的相关性,二者具有较好的线性关系,线性相关系数R²=0.96965。     

迷迭香提取物在油脂和肉类制品中的应用

本主要对迷迭香提取物和其他的抗氧化剂BHT,BHT,VE在油脂及肉类制品中抗氧化性做了研究与对比。     

抗氧化剂BHT和维生素E对菜籽油的抗氧化研究

油脂及富含油脂的食品易发生氧化酸败,为了防止其氧化最常见的方法是加入抗氧化剂.BHT和维生素E是两种常见的抗氧化剂.本文以酸价(AV)和过氧化值(POV)为指标,研究了单独使用BHT以及BHT和维生素E(VE)复配使用在(60±1)℃加速氧化下对菜籽油贮存稳定性的影响.研究结果表明:(1)在单独使用BHT作为抗氧化剂时,BHT对菜籽油有一定抗氧化性,其添加量为0.2‰时抗氧化效果最好,其抗氧化效果顺序为0.20‰＞0.05‰＞0.25‰ ＞0.15‰＞0.01‰;(2)在BHT和VE复配使用时比单独使用BHT效果好,其最佳配比浓度为0.10‰BHT和0.10‰VE,其抗氧化作用效果的顺序为0.10‰ BHT+0.10‰ VE＞0.15‰BHT +0.05‰VE＞0.10‰BHT +0.05‰VE ＞0.05‰BHT+0.05‰VE＞0.05‰BHT+0.10‰ VE＞0.10‰ BHT +0.15‰VE＞0.05‰BHT +0.15‰VE ＞0.15‰ BHT +0.15‰ VE ＞0.15‰ BHT+0.10‰ VE.     

Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity

We compared the phenolic metabolites and antioxidant activities of Thymus lotocephalus G. López & R. Morales wild plants and in vitro cultures using different extraction solvents. HPLC-DAD analysis allowed the identification and quantification of phenolic (caffeic and rosmarinic) acids and flavones (luteolin and apigenin) in extracts from both sources. The in vitro cultures accumulated large amounts of rosmarinic acid. However, extracts from both sources were able to neutralise free radicals in different test systems (TEAC and ORAC assays), to form complexes with Fe(2+) and to protect mouse brains against Fe(2+)-induced lipid peroxidation. The solvent significantly influenced the phenolic content and antioxidant activity of the extracts, water/ethanol being the most efficient for the extraction of antioxidant phytochemicals. We conclude that in vitro cultures of T. lotocephalus represent a promising alternative for the production of valuable natural antioxidants and an efficient tool for the in vitro biosynthesis of rosmarinic acid, therefore avoiding the need to exploit populations of wild plants.     

防老剂BHT的提纯及其纯度检测

为提高和准确测定防老剂BHT的纯度,采用重结晶和区域熔融法对工业品防老剂BHT进行提纯,并用高效液相色谱仪和差示扫描量热仪对样品进行纯度检测。高效液相色谱法(HPLC)的标准曲线法测得的回收率在100.19%~100.28%之间,相对标准差(RSD)为0.05%;提纯品防老剂BHT的纯度为99.09%,RSD为0.08%。用差示扫描量热法(DSC)测得提纯品纯度为99.16%,RSD为0.14%。纯度达到国际同类产品水平。     

Antioxidant capacity of medicinal plants from the Province of Córdoba (Argentina) and their in vitro testing in a model food system

The total phenols content (Folin–Ciocalteau assay) and antioxidant capacity (ferric reducing/antioxidant power – FRAP) of 41 plants from Córdoba (Argentina) were analyzed. Phenol content ranged from 8.2 to 100.2 mg GAE/g. FRAP ranged from 85.2 to 1862.0 μmol of Fe(II)/g. Capparis atamisguea had the lowest values of total phenols content and antioxidant capacity (8.2 mg GAE/g and 85.2 μmol of Fe(II)/g, respectively), while Ligaria cuneifolia exhibited the highest values (100.2 mg GAE/g and 1862.0 μmol of Fe(II)/g, respectively). A significant linear correlation (p < 0.05) was found (0.9125) between phenols content and antioxidant capacity. Results support the idea that these plants may be a good source of natural antioxidants for food applications. Plants from the Asteraceae family (the most representative of the Córdoba flora) were further tested for their DPPH radical scavenging activity. Some plant extracts were tested in a simple food system to investigate to their potential use in foods.     

BHT反应工艺和设备的改进

ＢＨＴ是一种通用型抗氧剂,在工业生产装置中有连续和间歇两种反应工艺流程,小规模装置多采用间歇搅拌釜式反应器,但存在着单位反应器容积生产能力低,异丙烯单耗高的缺陷。作者在新装置设计中,通过反应工艺和动力学特性分析,对反应工艺和设备进行改进。生产实践验证,这一改进有效地克服了原间歇反应工艺的缺陷．     

日粮添加BHT对饲喂氧化豆油黄羽肉鸡肌肉品质和抗氧化能力的影响

本试验旨在研究2,6-二叔丁基-4-甲基苯酚(BHT)对饲喂氧化豆油日粮黄羽肉鸡肌肉品质及抗氧化能力的影响。试验采用2×2因子设计,因素1为日粮中的豆油,按其品质分为新鲜豆油(MDA=13.4 nmol/mL)和氧化豆油(MDA=367.7 nmol/mL),因素2为日粮中BHT含量,按添加剂量分为两水平(0、125 mg/kg)。240羽1日龄优质黄羽肉鸡被随机分成4个处理组,每组6个重复,每个重复10只鸡。结果表明:与新鲜豆油组相比,氧化豆油组肌肉滴水损失和丙二醛MDA含量显著增加(p<0.05);肌肉内还原型谷胱甘肽(GSH)含量、总超氧化物歧化酶(T-SOD)活力和肌肉总抗氧化能力(T-AOC)显著降低(p<0.05);腿肌肌肉中γ-谷氨酰半胱氨酸合成酶调节亚单位(γ-GCLm)和γ-谷氨酰半胱氨酸合成酶催化亚单位(γ-GCLc)的mRNA表达量显著降低(p<0.05)。与不含BHT日粮组相比,日粮中添加125 mg/kg BHT能够显著降低肌肉中MDA含量、肌肉滴水损失及蒸煮损失(p<0.05),能够显著提高肌肉T-SOD和CAT酶活(p<0.05),显著提高肌肉中CAT和γ-GCLm的mRNA表达量(p<0.05)。结论:日粮中添加BHT能够通过提高黄羽肉鸡机体抗氧化能力,改善肉品质,缓解氧化豆油造成的氧化损伤。