Vital-Fluorochromization of Microorganisms Using 3′,6′-Diacetylfluorescein to Determine Damages of Cell Membranes and Loss of Metabolic Activity by Ozonation

Cell suspensions of Escherichia coli as a model for bacterial populations in wastewaters were treated with ozone as a disinfectant in a continuously operated pilot plant with a plug flow reactor. Suspensions with an initial number of CFU (colony forming units) of 10⁸ mL^?1 were ozonized with ozone concentrations up to 12 mg/L. Metabolic activities and membrane functions break down with increasing ozone concentrations. The fluorochromization using 3′,6′-diacetylfluorescein (FDA) proved to be a suitable method for the detection of an alteration in permeability of the cell membranes and an inactivation of metabolic activity. By fluorescence microscopic and photometric investigations it could be clearly demonstrated that, in the case of disinfection with ozone, inactivation of the cells includes first of all a damage of the cell membranes. In contrast to the determination of the number of CFU, fluorochromization allows the detection of alteration in metabolic activities.     

Second-law-based thermodynamic analysis of two-stage and mechanical-subcooling refrigeration cycles

Thermodynamic analysis of HFC-134a vapor-compression refrigeration cycles is investigated by both the first and second laws of thermodynamics. Second-law analysis is carried out for both two-stage and mechanical-subcooling refrigeration cycles. The analysis is performed on each of the system components to determine their individual contribution to the overall system irreversible losses. It is found that most of the losses are due to a low compressor efficiency. Irreversibilities of expansion valves and condenser are also significant. In addition, it is shown that the optimum inter-stage pressure for two-stage and mechanical-subcooling refrigeration systems is very close to the saturation pressure corresponding to the arithmetic mean of the refrigerant condensation and evaporation temperatures. These results are compared with the existing practice in the industry. Furthermore, theoretical results of a two-stage refrigeration system performance are also compared with experimental values for a CFC-22 system.     

Impact of uncertainties on estimations of heat pump cycle performance

Sensitivity analysis is used to show how uncertainties in basic factors, such as heat transfer, pressure drop, compressor efficiency, and thermophysical properties, influence the result of heat pump cycle performance estimations. By comparing the results of this analysis with uncertainty levels of estimations and experimental determinations, it is established to what extent improvements in accuracy are needed. The investigation was carried out for several different applications and, as working fluids, both pure substances and non-azeotropic mixtures were examined. It was found that evaporation heat transfer and compressor efficiency are two areas where improvements are needed. It is also shown that estimates using very accurate, substance-specific, equations of state differ from those using more general types by up to 1 % for COP and 2% for capacity.     

The antimicrobial effect of wine on Listeria innocua in a model stomach system

A model stomach, containing a food matrix and a synthetic gastric fluid, was used to study the bactericidal effect of ingested wine on Listeria innocua. Volumes of wine equivalent to the ingestion of one glass and half a bottle, led, over a period of less than 2 h, to a reduction of 3 and 4 logarithmic cycles of the initial population respectively. The influence of ethanol and organic acids, wine constituents with known antimicrobial properties, was investigated. Ethanol exhibited a higher bactericidal effect than the mixture of the main wine organic acids. When testing the organic acids separately, malic and lactic acids were found to have the strongest effect. The combination of ethanol with the organic acids acted synergistically but to a lesser extent than wine itself. The results suggest that the ingestion of wine during a meal may diminish the quantity of Listeria persisting further in the alimentary tract.     

粗甲醇在内燃机上的清洁高效使用

在内燃机排气废热的作用下。液体粗甲醇加压汽化后引入裂解器催化分解为富氢混合气，在上止点附近喷入气缸，发动机采用米勒循环，其功率与效率优于同等结构奥托发动机。     

人支气管上皮靶细胞形态学参数的测量

支气管上皮靶细胞形态学参数对计算受氡子体照射的支气管的剂量是很重要的。本文介绍利用奥尔新兰-PAS 染色的外科切除人肺组织切片,测量基底细胞粘液细胞和的胞核深度与数目。结果表明,叶、段和小支气管的粘液细胞核深度分别为相应节段基底细胞的61%、69%和73%;此三节段粘液细胞的数目分别为基底细胞的53%、29%和28%,平均为37%。     

压水堆核燃料循环的经济分析程序介绍

所开发的压水堆核燃料循环分析计算机经济程序包括12个子程序,代表着压水堆整个核燃料循环各种不同的工艺过程。本程序能算出压水堆核电站核燃料循环中燃料费用对发电成本的影响,给出各工艺过程对燃料成本的敏感度分析,并尽可能给出燃料循环中燃料材料及服务的价格数据.     

步进式加热炉汽化冷却节电初探

介绍了在首钢第一线材厂步进式加热炉汽化冷却设计中，以优化设计改进现有设备和技术，实现了高效节电的效果。     

Excel的常用函数在单元格中的粘贴要素

以十种函数为例,讨论了在EXCEL电子表格软件中的单元格里粘贴函数的应用要素,即参数的数量、参数的种类、参数之间的关系、函数的书写及粘贴过程等。例举少许易懂函数样例,有助于人们理解、发挥函数功能的强大作用,提高工作效率。     

吸入短寿命氡子体动物肺组织剂量与体重关系的探讨

本文介绍吸入短寿命氡子体的不同种动物肺组织吸收剂量与体重的关系。实验结果表明,吸收剂量随体重的增加而减小。