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51.
52.
Kang-Yi Lien Chien-Ju Liu Yi-Chien Lin Pao-Lin Kuo Gwo-Bin Lee 《Microfluidics and nanofluidics》2009,6(4):539-555
This study presents a new magnetic bead-based microfluidic platform, which integrates three major modules for rapid leukocytes
purification, genomic DNA (gDNA) extraction and fast analysis of genetic gene. By utilizing microfluidic technologies and
magnetic beads conjugated with CD15/45 antibodies, leukocytes in a human whole blood sample can be first purified and concentrated, followed by extraction of gDNA
utilizing surface-charge switchable, DNA-specific, magnetic beads in the lysis solution. Then, specific genes associated with
genetic diseases can be amplified by an on-chip polymerase chain reaction (PCR) process automatically. The whole pretreatment
process including the leukocytes purification and gDNA extraction can be performed in an automatic fashion with the incorporation
of the built bio-separators consisting of microcoils array within less than 20 min. The detection of single nucleotide polymorphism
(SNP) genotyping of methylenetetra-hydrofolate reductase (MTHFR) C677T region associated with an increased risk of genetic
diseases was further performed to demonstrate the capability of the proposed system. The extracted gDNA can be transported
into a micro PCR chamber for on-chip fast nucleic acid amplification of detection genes with minimum human intervention. Hence,
the developed system may provide a powerful automated platform for pretreatment of human leukocytes, gDNA extraction and fast
analysis of genetic gene. 相似文献
53.
Haiping Wu Shun Huang Ying Zhu Jia Li Xiaoxiao Pang Yongyao Tang Siqiao Li Ping Ji Shijia Ding Wei Cheng Wenyang Li 《Advanced functional materials》2023,33(19):2212669
The accumulation of reactive oxygen species (ROS) and minimal osteogenic raw material in the osteoporotic bone microenvironment greatly inhibits the activity of osteoblasts. Herein, it is originally proposed to construct a biomatrix multifaceted bone microenvironment amendment -Mineralized zippered G4-Hemin DNAzyme hydrogel (MDH)-to improve osteoporotic osteogenic capacity and promote high-quality bone defect repair. The programmed design of the rolling circle amplified DNA hydrogel synthesis system allows the introduction of massive amounts of zippered G4-Hemin DNAzyme in MDH. The zippered G4-Hemin DNAzyme highly mimics the tight catalytic configuration of horseradish peroxidase and exerts excellent enzyme-like activity with considerable ROS molecule scavenging ability. In addition, the DNA amplification by-product pyrophosphate is ingeniously employed as a sufficient phosphorus source, thus constituting an autonomous mineralization system for waste reuse through the introduction of pyrophosphate hydrolase and calcium ions, which deposits in MDH as an osteogenic raw material and addresses the challenge of DNA hydrogel bio-application stability. The remarkable in vitro and in vivo outcomes demonstrate that MDH can effectively improve the oxidative stress status of osteoblasts, restore the balance of mitochondrial membrane potential, and reduce apoptosis, ultimately demonstrating superior osteogenic capacity. 相似文献
54.
Manipulating Electrical and Fluidic Access in Integrated Nanopore‐Microfluidic Arrays Using Microvalves 下载免费PDF全文
55.
Takeo Uchida Keita Abe Yuma Endo Shosei Ichiseki Satoru Akita Shiyun Liu Sho Aradachi Masataka Saito Akihiko Fukuchi Taiyo Kikkawa Theo Dammaretz Ibuki Kawamata Yuki Suzuki Shin‐ichiro M. Nomura Satoshi Murata 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(41)
A new kind of the Vernier mechanism that is able to control the size of linear assembly of DNA origami nanostructures is proposed. The mechanism is realized by mechanical design of DNA origami, which consists of a hollow cylinder and a rotatable shaft in it connected through the same scaffold. This nanostructure stacks with each other by the shape complementarity at its top and bottom surfaces of the cylinder, while the number of stacking is limited by twisting angle of the shaft. Experiments have shown that the size distribution of multimeric assembly of the origami depends on the twisting angle of the shaft; the average lengths of the multimer are decamer, hexamer, and tetramer for 0°, 10°, and 20° twist, respectively. In summary, it is possible to affect the number of polymerization by adjusting the precise shape and movability of a molecular structure. 相似文献
56.
DNA Imaging: DNA Binding Peptide Directed Synthesis of Continuous DNA Nanowires for Analysis of Large DNA Molecules by Scanning Electron Microscope (Small 2/2017) 下载免费PDF全文
57.
58.
Gold Nanorods,DNA Origami,and Porous Silicon Nanoparticle‐functionalized Biocompatible Double Emulsion for Versatile Targeted Therapeutics and Antibody Combination Therapy 下载免费PDF全文
59.
DNA‐Metalization: Synthesis and Properties of Novel Silver‐Containing DNA Molecules (Adv. Mater. 24/2016) 下载免费PDF全文
60.
Imparting Designer Biorecognition Functionality to Metal–Organic Frameworks by a DNA‐Mediated Surface Engineering Strategy 下载免费PDF全文
Weiyu Ning Zhenghan Di Yingjie Yu Pingmei Zeng Chunzhi Di Daquan Chen Xueqian Kong Guangjun Nie Yuliang Zhao Lele Li 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(11)
Surface functionality is an essential component for processing and application of metal–organic frameworks (MOFs). A simple and cost‐effective strategy for DNA‐mediated surface engineering of zirconium‐based nanoscale MOFs (NMOFs) is presented, capable of endowing them with specific molecular recognition properties and thus expanding their potential for applications in nanotechnology and biotechnology. It is shown that efficient immobilization of functional DNA on NMOFs can be achieved via surface coordination chemistry. With this strategy, it is demonstrated that such porphyrin‐based NMOFs can be modified with a DNA aptamer for targeting specific cancer cells. Furthermore, the DNA–NMOFs can facilitate the delivery of therapeutic DNA (e.g., CpG) into cells for efficient recognition of endosomal Toll‐like receptor 9 and subsequent enhanced immunostimulatory activity in vitro and in vivo. No apparent toxicity is observed with systemic delivery of the DNA–NMOFs in vivo. Overall, these results suggest that the strategy allows for surface functionalization of MOFs with different functional DNAs, extending the use of these materials to diverse applications in biosensor, bioimaging, and nanomedicine. 相似文献