低频电子标签基带处理器的设计与实现

设计一款新型低频电子标签基带处理器,提出一种新颖的异步解码机制,解决了100% 振幅键控调制模式下时钟中断的问题,利用指令头解析与片选信号相结合的指令解析机制,提高了指令解析速度,并综合应用能量管理、结构优化等多种低功耗设计方法提高电子标签响应速度和抗干扰能力,降低芯片功耗与面积。该芯片采用SMIC 0.18 μm 2P4M标准CMOS工艺实现。与国外同类芯片相比,该设计以较小的面积与功耗实现了更高的性能。     

基于纳米材料的电化学生物传感器研究进展

该文主要从纳米尺寸材料电极的构建以及纳米材料作为生物分子指示剂两部分展开讨论,描述了依赖于阵列纳米管排列的生物分子与电极间的直接电子传递,纳米管、纳米颗粒为基质的纳米电极的构建,以及以金纳米颗粒、DNA量子点和蛋白为基础的多路分析技术与负载于CNT的新的纳米生物标记,特别讨论了纳米电化学方法在检测DNA和免疫传感器领域取得的研究进展.     

基于DNA技术的加密方案

DNA密码是伴随着DNA计算的研究而出现的密码学新领域。利用DNA合成技术、PCR扩增技术以及DNA数字编码技术,结合传统密码学提出了一种基于DNA技术的加密方案。方案利用引物对于PCR扩增技术的特殊作用,提出要以引物和编码方式为密钥,采用传统的加密方法对明文进行加密预处理,可有效防止可能词作为PCR引物进行攻击。生物学困难问题和密码学计算困难问题为该方案提供了双重的安全保障,安全性分析表明该加密方案具有很强的保密强度。     

基于动态连接池的数据集成中间件的设计与实现

介绍数据集成中间件技术的基本概念与体系结构,分析现有数据库连接池的工作原理和工作机制.在得出现有连接池局限性的基础上,设计出动态连接池体系结构.通过采用基于中间数据库和变化日志表的异构数据复制模式和以虚拟订阅表的方式构建的全局查询模式,将动态连接池中间件扩展成为数据集成中间件,从而有效解决异构数据库间的数据共享与交换问题.     

一种基于现场总线的高效率双机热备实现方案

讨论了双机热备技术并着重探讨了一种基于总线的双机热备方式的优点及实现,并介绍了此方法在闸门群集控中的应用。此方法简单易行、技术可靠、实时性高,硬件又不需要增加额外投资,在工控领域具有很高的推广应用价值。     

一种基于模糊聚类的构造进化树方法

各种生物之间的进化史可以通过构建进化树来讨论,因此进化树的研究成了一个研究热点。提出将利用DNA序列的4D表示所得相似矩阵视为模糊矩阵,再利用最大树法来构建进化树的方法。该方法不需要多序列比对,计算简单,实验验证了该方法的有效性。     

基于改进非支配遗传算法的DNA编码序列优化方法

针对DNA计算中的编码序列设计问题,分析了DNA编码序列设计的目标和需要满足的约束条件,并建立了相应的数学模型。通过将约束条件引入非支配排序过程,提出了一种改进的NSGA-Ⅱ算法。实验结果表明,该算法具有良好的收敛特性和种群多样性,能为可控的DNA计算提供可靠的编码序列。     

Molecular modeling of tricyclic compounds with anilino substituents and their intercalation complexes with DNA sequences

Although 9-anilinoacridines are among the best studied antitumoral intercalators, there are few studies about the effect of isosteric substitution of a benzene moiety for a heterocycle ring in the acridine framework. According to these studies, this approach may lead to effective cytotoxic agents, but good cytotoxic activity depends on structural requirements in the aniline ring which differ from those in 9-anilinoacridines. The present paper deals with molecular modeling studies of some 9-anilino substituted tricyclic compounds and their intercalation complexes (in various DNA sequences) resulting from docking the compounds into various DNA sequences. As expected, the isosteric substitution in 9-anilinoacridines influences the LUMO energy values and orbital distribution, the dipole moment, electrostatic charges and the conformation of the anilino ring. Other important differences are observed during the docking studies, for example, changes in the spatial arrangement of the tricyclic nucleus and the anilino ring at the intercalation site. Semiempirical calculations of the intercalation complexes show that the isosteric replacement of a benzene ring in the acridine nucleus affects not only DNA affinity but also base pair selectivity. These findings explain, at least partially, the different structural requirements observed in several 9-anilino substituted tricyclic compounds for cytotoxic activity. Thus, the data presented here may guide the rational design of new agents with different DNA binding properties and/or a cytotoxic profile by isosteric substitution of known intercalators.     

Extraction of genomic DNA and detection of single nucleotide polymorphism genotyping utilizing an integrated magnetic bead-based microfluidic platform

This study presents a new magnetic bead-based microfluidic platform, which integrates three major modules for rapid leukocytes purification, genomic DNA (gDNA) extraction and fast analysis of genetic gene. By utilizing microfluidic technologies and magnetic beads conjugated with CD_15/45 antibodies, leukocytes in a human whole blood sample can be first purified and concentrated, followed by extraction of gDNA utilizing surface-charge switchable, DNA-specific, magnetic beads in the lysis solution. Then, specific genes associated with genetic diseases can be amplified by an on-chip polymerase chain reaction (PCR) process automatically. The whole pretreatment process including the leukocytes purification and gDNA extraction can be performed in an automatic fashion with the incorporation of the built bio-separators consisting of microcoils array within less than 20 min. The detection of single nucleotide polymorphism (SNP) genotyping of methylenetetra-hydrofolate reductase (MTHFR) C677T region associated with an increased risk of genetic diseases was further performed to demonstrate the capability of the proposed system. The extracted gDNA can be transported into a micro PCR chamber for on-chip fast nucleic acid amplification of detection genes with minimum human intervention. Hence, the developed system may provide a powerful automated platform for pretreatment of human leukocytes, gDNA extraction and fast analysis of genetic gene.