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61.
62.
本文合成了以Eu3+为中心离子,芳香羧酸、邻菲咯啉和噻酚甲酰三氟丙酮为第一配体,油酸为第二配体的七种新的铕三元荧光配合物。通过红外光谱分析、紫外光谱分析、元素分析、EDTA配位滴定分析以及电导率的测定对铕三元荧光配合物的组成及结构进行表征。通过荧光光谱分析,确定了铕三元荧光配合物的最佳激发波长,在最佳激发波长下测定了它们的发射光谱。结果表明,所有铕三元荧光配合物的荧光发射光谱均相似,发出了铕离子的5D0-7F2,5D0-7F1,5D0-7F0(很弱)特征光,荧光表现为红色。在含油酸配体的铕三元荧光配合物中,各第一配体向铕离子传递光能的能力为:邻菲咯啉>噻吩甲酰三氟丙酮>大茴香酸>间氯苯甲酸>苯甲酸>对甲基苯甲酸>对羟基苯甲酸。 相似文献
63.
探讨荧光原位杂交技术预测人大肠癌细胞内在放射敏感性的可行性.应用荧光原位杂交技术检测人大肠癌动物模型照射后癌细胞2号染色体易位畸变并分析其与照射后肿瘤生长曲线的相关性.结果表明,放疗使Lovo移植瘤的增长明显延缓,Lovo细胞的放射敏感性高于SW480细胞.X射线照射后肿瘤荧光原位杂交技术检测结果显示:Lovo细胞的2号染色体诱导易位畸变量显著大于SW480细胞(p<0.05).荧光原位杂交技术能快捷、可靠地预测人大肠癌细胞的放射敏感性,从而为临床大肠癌病人放疗的选择提供重要参考依据. 相似文献
64.
Determination of L3 subshell X-ray production cross-sections and fluorescence yields of Pb, Th and U
nder
im
ek 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2000,170(3-4):293-298
A study of L3 subshell X-ray production cross-sections and fluorescence yields by using characteristic K X-rays as the exciting radiation is described. Only the L3 subshells of Pb, Th and U were excited by the characteristic K X-rays of the elements used as secondary sources. The cross-sections for the production of Ll, L and Lβ groups of L3 subshell X-rays of Pb, Th and U have been measured using photoionization by Rb, Nb and Mo K X-rays. The measured L3 X-ray production cross-section values for Pb, Th and U are in good agreement with the theoretical ones evaluated using L3 subshell fluorescence yield ω3, fractions of the radiative width F3i and L3 subshell photoionization cross-section σ3. The L3 subshell fluorescence yields (ω3) have also been computed using the presently measured cross-section values and the theoretical L3 subshell photoionization cross-section values. These results are compared with the theoretically predicted values. 相似文献
65.
《Ceramics International》2020,46(7):8928-8934
Multifunctional nanomaterials composed of magnetic and fluorescent nanoparticles have been one of the most extensive pursuits because of the potential application in bio-research. In this paper, we demonstrated an efficient method by coupling CdSe/CdS/ZnS quantum dots (QDs) with Fe3O4 magnetic nanoparticles(MNPs) while functionalized multiwall carbon nanotubes (f-MWCNTs) were used as matrix to synthesize a kind of magnetic fluorescent nanocomposite. Compared with other matrix materials, carbon nanotubes have the advantages of high surface areas and good biocompatibility. The incorporation of f-MWCNTs supplies plenty of nucleation sites for the preferential growth of Fe3O4 nanoparticles, avoiding the agglomeration phenomenon of Fe3O4 MNPs in traditional co-precipitation method. Moreover, the un-reacted functional groups of f-MCNTs can further adsorb biological species and drugs, averting the decline of fluorescent intensity caused by the modification of biological species and drugs. The synthetic product maintains the unique properties of rapid magnetic response and efficient fluorescence, which shows a broad application prospect in fluorescent labeling, biological imaging, cell tracking and drug delivery. 相似文献
66.
提出了一种采用基于电流模的放大器和滤波器电路设计方法,并利用第二代电流传输器CCⅡ实现了三阶巴特沃思低通电流模滤波器。在对第二代电流传输器结构和原理进行分析的基础上,利用CCⅡ设计了三阶巴特沃思低通电流模滤波器,并把R和C提取到CCⅡ芯片外面使它更好应用于低频荧光信号测量。电路经过Pispice的仿真证明了电路的可行性,从而提高了荧光信号检测电路的速度和信噪比,使得检测精度大大提高。本文提出的方法正确、原理清楚、电路可行。并且,电流模技术为微弱荧光信号的低噪声测量、放大和滤波提供了一条可行的技术路线。 相似文献
67.
The effect of pH on the competition between polyphosphate-accumulating organisms and glycogen-accumulating organisms 总被引:24,自引:0,他引:24
In enhanced biological phosphorus removal (EBPR) processes, glycogen-accumulating organisms (GAOs) may compete with polyphosphate-accumulating organisms (PAOs) for the often-limited carbon substrates, potentially resulting in disturbances to phosphorus removal. A detailed investigation of the effect of pH on the competition between PAOs and GAOs is reported in this study. The results show that a high external pH ( approximately 8) provided PAOs with an advantage over GAOs in EBPR systems. The phosphorus removal performance improved due to a population shift favouring PAOs over GAOs, which was shown through both chemical and microbiological methods. Two lab-scale reactors fed with propionate as the carbon source were subjected to an increase in pH from 7 to 8. The phosphorus removal and PAO population (as measured by quantitative fluorescence in situ hybridisation analysis of "Candidatus Accumulibacter phosphatis") increased in each system, where the PAOs appeared to out-compete a group of Alphaproteobacteria GAOs. A considerable improvement in the P removal was also observed in an acetate fed reactor, where the GAO population (primarily "Candidatus Competibacter phosphatis") decreased substantially after a similar increase in the pH. The results from this study suggest that pH could be used as a control parameter to reduce the undesirable proliferation of GAOs and improve phosphorus removal in EBPR systems. 相似文献
68.
An experimental study has been performed to detect the occurrence of nanosized carbon particulates below the soot laden zone of a co-flowing partially premixed flame. Samples have been extracted from different points across the flame and passed through DI water. Absorption and fluorescence spectroscopies have been performed with the collected water suspensions. The occurrence of carbon nanoparticles is evident across the inner flame front. In addition, evidence of naphthalene has also been found inside the inner rich premixed flame. The concentration of naphthalene decreases while that of the carbon nanoparticles increases as the inner flame front is reached. The stability of the nanoparticles in the sample has been ensured by observing that the change in fluorescence quantum yield from the sample over a long duration is small. The band gap energy has been evaluated using the absorption data to characterize the likely structures of the particles in the collected suspension. Two kinds of particles having different zones of band gap energy are found in the flame. Dynamic light scattering measurements show that the particle size grows with the increase in height in the lower part of the flame. While, at 3 and 6 mm elevations the particles are observed to be below 2.5 nm in diameter, the particles at 10 mm elevation are found in the size range of 2.5-5.5 nm. 相似文献
69.
70.
W. Liou 《Journal of microscopy》1990,157(2):239-245
A technique is given for the preparation of a sheet of epithelial cells from the capsule of the crystalline lens. A new method is described for fixation and staining with fluorescent phalloidin or actin antibody in order to localize the actin cytoskeleton in this tissue. Optical section of the preparation resolves such actin features as apical polygonal arrays, sequestered actin bundles, perinuclear actin aggregates, observed here for the first time, and filamentous networks in the basal region of the cell. This method is superior to previous ones in its ability to preserve actin-abundant sectors distinctively. 相似文献