亚麻酸对乳蛋白理化性质与免疫球蛋白G/E结合能力的影响

目的 探索亚麻酸对乳蛋白理化性质与免疫球蛋白(immunoglobulin,Ig) G/E (IgG/IgE)结合能力的影响。方法 以α-乳白蛋白和β-乳球蛋白为实验材料,通过非还原性十二烷基硫酸钠聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)、扫描电镜、粒径以及Zeta电位、间接竞争酶联免疫吸附试验法(enzyme-linked immunosorbent assay,ELISA)评价亚麻酸对乳蛋白理化性质以及IgG/IgE结合能力的影响。结果 亚麻酸能够增强乳蛋白的IgG/IgE结合能力,并且通过扫描电镜、粒径以及Zeta电位结果发现,亚麻酸的加入导致α-乳白蛋白和β-乳球蛋白的颗粒变大,粒径变大,并且稳定性更强,说明亚麻酸诱导乳蛋白发生了分子间的聚合,形成高聚物。结论 亚麻酸可诱导α-乳白蛋白和β-乳球蛋白形成聚合物,并能促进IgG/IgE结合能力增强。     

Covalent immobilization of antibodies for the preparation of immunoaffinity chromatographic supports

Immunosorbents in immunoaffinity chromatography (IAC) are prepared by immobilizing expensive antibodies without guidelines for ensuring the best coupling efficiencies, and avoiding low binding capacities. Covalent immobilization of antibodies on N-hydroxysuccinimide (NHS)-activated Sepharose 4 Fast Flow resin was optimized using human IgG via full factorial design with incubation times (4, 9, 14, 19 and 24 h), temperatures (4°C and 20°C) and coupling reaction buffers (sodium bicarbonate and triethanolamine). The best coupling efficiency (CE) (83.4 ± 8.7%) was reached with triethanolamine buffer, 14 h and 4°C. Comparison of antibody isotypes (IgG or IgM) by a nested factorial analysis suggested that antibodies in the IgG isotype presents the best coupling efficiency.     

静脉滴注丙种球蛋白治疗急性毛细支气管炎的研究

应用静脉滴注丙种球蛋白（IVIg）治疗毛细支气管炎25例,以30例应用新鲜血浆或血液者为对照,结果无论是临床症状、体征,还是体液免疫功能变化,均较对照组好转、恢复快,并且没有出现任何后遗症和死亡病例。     

壳聚糖／明胶磁性微球的反相悬浮乳液包埋法制备研究

在油包水（W／O）体系中,采用反相悬浮乳液包埋法,以戊二醛为交联剂,实现了壳聚糖和明胶（Gs／Gel）微球对Fe304磁性粒子的包裹．其制备的磁性微球粒径在3～8μm范围,分散性和磁响应性良好;磁性微球可较好地吸附BSA和亲和层析兔抗β-CNIgG,对后者的吸附率在48h稳定后可达到42．75％,为实验室制备高质量免疫磁性微球奠定了基础．     

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 µg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies.     

A study on indirect radiolabeling of IgG with carrier free ~(188)Re

188Re labeled monoclonal antibodies are potential candidates for use in radioimmunotherapy. S-Bz-MAG3 as a bifunctional chelating agent was used for labeling of IgG with carrier free 188Re by pre-radiolabel-ing of the chelating approach. The conjugation conditions were optimized. The stability of 188Re-MAG3-IgG in vitro was high. The results may be useful to the studies of 188Re labeled MAbs for radioimmunotherapy.     

人巨细胞病毒ISCOMs的研制

应用巨细胞病毒被膜蛋白与Quil A结合制备ISCOMs疫苗,经小鼠的免疫学实验发现,ISCOMs实验组IgG及IgM水平明显高于其它对照组,其IgG1、IgG2a、IgG2b也较其它组为高。提示该疫苗有可能在预防人体HCMV原发感染中发挥作用。     

He-Ne激光照射对小鼠血清IgG的影响

目的：研究低能量激光对小鼠免疫功能的影响，方法：以不同剂量的He-Ne激光照射小鼠，分阶段测试小鼠血清IgG浓度，并与对照组对比分析。结果：中等剂量He-Ne激光照射可提高小鼠血清IgG水平，改善其免疫状态。结论：激光照射可改善机体体液免疫水平。     

IgG Antibody from Hen Egg Yolks: Purification by Ethanol Fractionation

A procedure was developed for large-scale preparation of IgG antibodies from egg yolks. The supernatant from egg yolks was obtained after an initial 9–fold dilution with water. The lipids in the supernatant were then almost completely eliminated from the water-soluble protein fraction containing the antibody, by precipitation with 60% ethanol and filtration. Yolk antibody was purified from the lipid-free water-soluble protein fraction by ethanol fractionation at final concentration 30% (pH unadjusted), and again at 25% (pH 7.4). The purified fraction was composed of >99% pure IgG. Recovery of antibody was calculated as 40%.     

The anti‐allergenic properties of milk kefir and soymilk kefir and their beneficial effects on the intestinal microflora

Food allergy is now recognized as a worldwide problem, and like other atopic disorders its incidence appears to be increasing. Kefir is reported to possess the ability to reduce intestinal permeation of food antigens; however, no experimental study has clearly evaluated the relationships between kefir consumption, allergen‐specific IgE response, and intestinal microflora. The aim of this study was to evaluate the effect of oral consumption of milk kefir and soymilk kefir on in vivo IgE and IgG1 production induced by ovalbumin (OVA) in mice. The effects of kefir administration on the murine intestinal microflora were also examined. Oral administration of milk kefir and soymilk kefir for 28 days significantly increased the fecal populations of bifidobacteria and lactobacilli, while it significantly decreased those of Clostridium perfringens. Milk kefir and soymilk kefir also significantly decreased the serum OVA‐specific IgE and IgG1 levels for both groups, but not those of the IgG2a analogues. Consumption of milk kefir and soymilk kefir suppressed the IgE and IgG1 responses and altered the intestinal microflora in our supplemented group, suggesting that milk kefir and soymilk kefir may be considered among the more promising food components in terms of preventing food allergy and enhancement of mucosal resistance to gastrointestinal pathogen infection. Copyright © 2006 Society of Chemical Industry