W6Mo5Cr4V2/A100复合材料的制备及摩擦性能

为了提高A100钢的摩擦学性能,采用气雾化法制备纯A100钢粉末及分别添加10％、17％、23％W6Mo5Cr4V2高速钢的W6Mo5Cr4V2/A100合金粉末,再利用粉末冶金工艺制备W6Mo5Cr4V2/A100复合材料.研究不同含量W6Mo5Cr4V2钢对复合材料力学性能和摩擦性能的影响,并探讨其磨损机理.结果表...     

X100管线钢双丝埋弧焊接头微观组织与力学性能

利用光学显微镜、扫描电子显微镜、拉伸及冲击材料试验机等对X100钢级管线钢组织与力学性能进行了研究.结果表明,X100钢级管线钢焊缝区组织主要为针状铁素体和粒状贝氏体,热影响区组织有多边形铁素体存在,且晶粒粗大,发生了软化和脆化.焊接接头最高抗拉强度和断后伸长率分别达805 MPa,10.7%.接头的冲击吸收功(-10℃)大于110 J;剪切面积百分比(-10℃)平均值达到85%,呈现为韧性断裂.硬度测试结果显示,热影响区硬度外焊高于内焊.     

石墨材料在无油润滑领域的应用研究

采用石墨为主要原料,添加适量的二硫化钼和氟化石墨,制成高效固体润滑材料,应用于无油润滑轴承中,其各项性能指标达到日本Ｓａｎｋｙｏ公司和Ｏｉｌｌｅｓｓ公司同类产品的水平。     

Microstructure and corrosion performance of X100 steel in the oilfield produced water

ABSTRACT

The corrosion performances of X100 steels in the oilfield produced water with different temperature (30°C, 40°C, 60°C) under the simulated working conditions were studied by potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) test. Energy dispersive spectroscopy (EDS) was used to measure the element composition of the sample after corrosion. The phase and chemical compositions of the specimens were analyzed by X-ray diffraction (XRD) and Raman spectrometry. The electrochemical results showed that the corrosion resistance of the specimen exposed for 24 hours increased with the increase of the testing temperature, indicating the better protective effect due to the corrosion product film formed under high temperature despite the impact of turbulence. The corrosion products consist of Fe₃O₄, Fe₂O₃, α-FeOOH, and γ-FeOOH. The corrosion mechanism model of X100 steel was established and the corrosion behavior was analyzed under simulated working conditions.     

Contributing to Understand the Crosstalk between Brain and Periphery in Methylmercury Intoxication: Neurotoxicity and Extracellular Vesicles

Human exposure to methylmercury (MeHg) is currently high in regions such as the Amazon. Understanding the molecular changes associated with MeHg-induced neurotoxicity and the crosstalk with the periphery is essential to support early diagnoses. This work aimed to evaluate cellular and molecular changes associated with behavioral alterations in MeHg acute exposure and the possible changes in extracellular vesicles (EVs) number and S100β content. Adults male Wistar rats were orally treated with 5 mg/kg for four days. Behavioral performance, molecular and histological changes in the cerebellum, and plasma EVs were assessed. MeHg-intoxicated animals performed significantly worse in behavioral tests. MeHg increased the number of GFAP+ cells and GFAP and S100β mRNA expression in the cerebellum but no change in NeuN+ or IBA-1+ cells number was detected. The number of exosomes isolated from plasma were decreased by the metal. S100B mRNA was detected in circulating plasma EVs cargo in MeHg exposure. Though preliminary, our results suggest astrocytic reactivity is displaying a protective role once there was no neuronal death. Interestingly, the reduction in exosomes number could be a new mechanism associated with MeHg-induced neurotoxicity and plasma EVs could represent a source of future biomarkers in MeHg intoxication.     

Molecular modeling of single polypeptide chain of calcium-binding protein p26olf from dimeric S100B()

P26olf from olfactory tissue of frog, which may be involvedin olfactory transduction or adaptation, is a Ca²⁺-binding proteinwith 217 amino acids. The p26olf molecule contains two homologousparts consisting of the N-terminal half with amino acids 1–109and the C-terminal half with amino acids 110–217. Eachhalf resembles S100 protein with about 100 amino acids and containstwo helix–loop–helix Ca²⁺-binding structural motifsknown as EF-hands: a normal EF-hand at the C-terminus and apseudo EF-hand at the N-terminus. Multiple alignment of thetwo S100-like domains of p26olf with 18 S100 proteins indicatedthat the C-terminal putative EF-hand of each domain containsa four-residue insertion when compared with the typical EF-handmotifs in the S100 protein, while the N-terminal EF-hand ishomologous to its pseudo EF-hand. We constructed a three-dimensionalmodel of the p26olf molecule based on results of the multiplealignment and NMR structures of dimeric S100B(ßß)in the Ca²⁺-free state. The predicted structure of the p26olfsingle polypeptide chain satisfactorily adopts a folding patternremarkably similar to dimeric S100B(ßß). Each domainof p26olf consists of a unicornate-type four-helix bundle andthey interact with each other in an antiparallel manner formingan X-type four-helix bundle between the two domains. The twoS100-like domains of p26olf are linked by a loop with no sterichindrance, suggesting that this loop might play an importantrole in the function of p26olf. The circular dichroism spectraldata support the predicted structure of p26olf and indicatethat Ca²⁺-dependent conformational changes occur. Since theC-terminal putative EF-hand of each domain fully keeps the helix–loop–helixmotif having a longer Ca²⁺-binding loop, regardless of the four-residueinsertion, we propose that it is a new, novel EF-hand, althoughit is unclear whether this EF-hand binds Ca²⁺. P26olf is a newmember of the S100 protein family.     

An analytical approach to reveal the addition of heat-denatured whey proteins in lab-scale cheese making

A simple analytical procedure for the detection of self-aggregated heat-denatured whey proteins (HDWP) in model cheeses was developed. The principle of the approach lies in the solubilization of the cheese matrix by a sodium citrate solution (0.2 M, pH 7.0) resulting in the dissociation of the casein micelles and the insolubilization of HDWP aggregates, which are collected in the pellet after a centrifugation step. The reliability of the procedure was tested in lab-scale cheeses from peroxidase-positive pasteurized milk with different protein-based ingredients (microparticulated whey protein concentrate, milk protein concentrate, whey protein isolate and Ricotta cheese) at concentrations ranging from 0.2 to 1.2% protein (w/v on cheese milk). A linear relationship between the amount of the HDWP added to cheese milk and that recovered from model cheeses was observed. Heat-damage indicators, furosine and lysinoalanine, showed levels in the experimental cheese samples not related with added HDWP, but represented a source of information on the ingredients other than liquid milk. Overall, in the model cheeses, the proposed method was an easy-to-apply and reliable tool for the evaluation of the presence of HDWP-based products. Further investigation is required for the application to real cheeses and for the evaluation of possible interferences from proteolysis during ripening.     

X100管线钢应变时效行为研究

通过不同温度和预应变量下X100管线钢的应变时效试验,对比了应变时效前后X100管线钢力学性能和显微组织的变化,研究了应变时效对X100管线钢力学性能和金相组织的影响。研究结果表明,应变时效可使X100管线钢的强度、屈强比和韧脆转变温度升高;应变时效对X100管线钢纵向力学性能的影响比横向大;应变时效可使X100管线钢组织细化,并且随着应变量的增大,加剧了应变时效的影响。     

X100管线钢埋弧焊用烧结焊剂的研制

介绍了X100管线钢埋弧焊用烧结焊剂的渣系选择,分析了碱度范围确定及焊接工艺性和力学性能,通过与X100匹配焊丝H03MnNi3MoTiB进行双丝埋弧焊接试验,其焊缝和热影响区质量均满足API SPEC 5L标准要求,但焊道表面存在黑斑,经能谱分析得知焊道表面黑斑成分为氧化亚铁,并设想提出通过增强熔渣的保护作用和降低焊缝氧含量进而消除黑斑现象的方案,从而得到优异的X100管线钢焊剂配方。