黑果枸杞花青素对Aβ_(42)致痴呆模型大鼠记忆力及抗氧化活性研究

采用双侧海马CA1区注射Aβ42构建阿尔茨海默症(Alzheimer′s disease,AD)动物模型,通过穿梭箱被动回避记录行为学数据;以大鼠血清和脑组织中的总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活力及还原型谷胱甘肽(GSH)、丙二醛(MDA)和蛋白质羰基(PC)含量作为评价指标,分析黑果枸杞花青素(OPC)样品对AD模型大鼠记忆力以及体内抗氧化活性的影响。结果表明,模型组动物的记忆能力显著下降,而黑果枸杞花青素组可改善AD模型大鼠的记忆损伤;同时,发现灌胃剂量为80 mg/kg的黑果枸杞花青素组能显著提高AD大鼠血清和脑组织中T-SOD、CAT活力和GSH含量,并降低MDA和蛋白质羰基含量水平。综合上述结果可知,本研究所述黑果枸杞花青素具有良好的增强体内抗氧化活性和提高AD大鼠记忆力的作用,并具有预防AD的潜在功效。     

SO_4~(2-)/SnO_2-高岭土催化合成食用香精丙酸正丁酯

使用价格相对便宜的锡类化合物和高比表面积的高岭土,采用超声波沉淀及微波干燥方法制得性价比较高的固体超强酸催化剂SO42-/SnO2-高岭土,并应用于食用香精丙酸正丁酯的合成反应。考察了该酯化反应中酸醇物质量之比、催化剂用量、反应时间、带水剂种类及用量等因素对酯化率的影响、并对催化剂的重复使用情况进行了考察。得到最佳反应条件:醇酸摩尔比为2∶1,催化剂用量为反应物料总量的3%,反应温度114¹16℃,正丁醇作带水剂,反应时间为4 h,此时酯化率达97.02%,高于多数文献值,催化剂重复使用第3次酯化率仍达到86.58%,经GCMS分析知产品纯度达96.68%。     

Synthesis and purification of trisulphoindigo and reversed-phase high performance liquid chromatographic determination of trisulphoindigo in FD & C Blue No.2

The effect of temperature on product formation during the sulphonation of indigo with concentrated sulphuric acid and 30 % fuming sulphuric acid was studied with the aid of high performance liquid chromatographic (HPLC) analysis. Trisulphoindigo was prepared by the sulphonation of indigo with concentrated sulphuric acid at 160°C. The di- and tetra-sulphoindigo side products formed during the reaction were removed by preparative HPLC to yield a product of high chromatographic purity. The trisulphoindigo content of the food dye FD & C Blue No. 2 and the purity of commercially available trisulphoindigo were determined by HPLC analysis.     

Investigation on microstructures and electrochemical performances of hydrogen storage alloys

In order to improve the cycle stability of La–Mg–Ni system (A₂B₇-type) alloy electrode, a small amount of Co was added in the La_0.75Mg_0.25Ni_3.5 alloy. The effects of Co content on the microstructures and electrochemical performances of the alloys were investigated in detail. The results by XRD and SEM show that the alloys have a multiphase structure which is composed of the LaNi₅, (La,Mg)₂Ni₇ two major phases and a small amount of the LaNi₂ phase. The cell volumes of the LaNi₅ and phases enlarge with the increasing Co content in the alloys. With the increasing Co content, some electrochemical properties and kinetic parameters of the alloy, involving the discharge capacity, high-rate discharge ability (HRD), the polarization resistance (R_p), the loss angle (ψ) and the limiting current density (I_L), first increase and then decrease. The addition of Co slightly improves the cycle stabilities of the alloy electrodes. The mechanism of the efficiency loss of the experimental alloy was investigated by means of SEM and X-ray photoelectron spectroscopy (XPS). The results indicate that the fundamental reasons for the capacity decay of the La_0.75Mg_0.25Ni_3.5Co_x (x=0,0.2,0.4,0.6) alloy electrodes are the pulverization of the alloy particle and corrosion/oxidation of La and Mg in alkaline electrolyte.     

Ultra-Small ATP-Decorated Gold Nanoparticles for Targeting Amyloid Fibrils in Neurodegenerative Diseases

Ultrafine Gold nanoparticles (Au NPs) functionalized with various biomolecules constitute an alternative to antibodies as anti-amyloidogenic agents. However, generating stable ultrafine Au NPs with high surface activity is challenging. Here, the capacity of phosphate groups in biomolecules is used to stabilize Au NPs. The characteristics of Au NPs decorated with adenosine mono-, di-, and tri-phosphate are compared as well as adenosine and peptide nucleic acid-containing adenosine as controls. Among them, ATP-Au NPs are found to be superior having small size (2–4 nm) and stability (for several months) when analysed by spectroscopy and electron microscopy. Spectroscopy analysis also revealed that each ATP-stabilized Au NP is decorated with 7–8 molecules of ATP. ThT binding analysis and TEM imaging showed that the ATP-Au NPs efficiently prevented amyloid fibril formation in vitro by Aβ-42, α-Synuclein as well as by the Glucosylceramide metabolite, and disaggregated their pre-formed fibrils. NMR analysis revealed the interaction of the ATP-Au NPs with the amyloid fibrils. The ATP-Au NPs are safe toward cultured SH-SY5Y cells and when co-incubated with α-Synuclein amyloids inhibited their cytotoxicity and readily enter the cells to inhibit formation of amyloid fibrils within them. The results indicates the pharmacological potentials of ATP decorated Au NPs.     

镀镍金属化42CrMo钢的微观组织与传感特性研究

采用化学镀镍和电镀镍相结合的方法,对光纤光栅传感器进行保护,然后用钎焊方式将传感器嵌入42Cr Mo钢基体中获得智能金属结构,对其微观组织和传感特性进行研究。结果表明,智能金属结构的灵敏度大大提高,比光纤光栅的灵敏度高1倍。     

切削用量对车削42CrMo钢切削力的影响

在单因素条件下通过改变切削用量车削42CrMo钢试验,得出了切削用量的改变对主切削力、进给力、背向力与总切削力的影响规律,分析了切削用量的改变导致切削力变化的原因,提供了车削42CrMo钢半精加工阶段的参考切削用量。结果表明:背吃刀量和进给量的改变对主切削力的影响较大,而切削速度的改变对主切削力影响较小;总切削力的变化规律与主切削力相似,背吃刀量对进给力的影响较大。     

42CrMo钢加热时奥氏体晶粒长大演化规律

对42CrMo钢在不同加热温度(850~1150℃)和保温时间(0~1200 s)下的奥氏体晶粒长大规律进行了研究。采用金相定量法对加热后材料的奥氏体晶粒度进行测量,建立42CrMo钢加热时奥氏体晶粒长大演化模型。结果表明:奥氏体晶粒尺寸随加热温度升高呈指数关系长大,随保温时间的延长呈近似抛物线形式长大;利用基于唯象理论的Sellars模型,通过非线性回归方法建立42CrMo钢加热时奥氏体晶粒长大演化模型;将该模型导入有限元软件中预报42Cr Mo钢加热时奥氏体晶粒变化,预报结果与实验结果吻合,验证了该模型的正确性。     

1500MPa级高强度钢42CrMoVNb的氢吸附行为

采用阴极充H和H热分析等实验方法,研究了新开发的1500 MPa级高强度钢42CrMoVNb在不同奥氏体化温度淬火和不同温度回火后的H吸附行为,并与常用钢42CrMo进行了对比.结果表明,在淬火态及不同温度回火处理后,42CrMoVNb钢充H试样的H逸出曲线峰值温度θp在200-250℃之间.回火温度从200℃升高到5...     

120装载机履带节以铸代锻的研究

介绍了用ZC-42CrMo材质，以精密铸造的方法，代替通常使用的50Mn材质、锻造方法生产的120立爪装载机履带总成的履带节的研制和应用情况。结果表明，该方法不仅满足履带节力学性能及使用性能要求，而且生产成本显著降低。