6C型肺炎链球菌抗血清的制备及鉴定

目的制备6C型肺炎链球菌抗血清,并进行鉴定。方法将经美国单克隆抗体方法鉴定为6C型的肺炎链球菌制成菌体抗原免疫家兔,制备抗血清;将抗血清与6A、6B和6C型肺炎链球菌进行荚膜肿胀和凝集试验;用6A、6B及6A和6B型肺炎链球菌吸附血清后,再次检测其与各型肺炎链球菌的凝集效价。结果免疫前的兔血清与6A、6B和6C型肺炎链球菌均未出现荚膜肿胀及凝集反应。免疫后的血清与6A、6B和6C型的凝集效价分别为1∶64、1∶32和1∶64;经6A或6A和6B型吸附后,与6A、6B和6C型的凝集效价分别为(-)、(-)和1∶8;经6B型吸附后,与6A、6B和6C型的凝集效价分别为1∶16、(-)和1∶16。结论6C型为新的肺炎链球菌血清型,已成功制备出6C型抗血清,可用于血清分型研究。     

RAC-OVA抗原和抗RAC单克隆抗体的筛选

在进行酶联免疫反应实验操作过程中,为了保证材料质量及使用的延续性,在使用前分别对两对抗原抗体进行了筛选和评价.主要是从抗体灵敏度的测定、抗体特异性的测定和抗体效价的测定这三个方面采选择灵敏度好、特异性强和效价高的一对抗原抗体.其中抗体灵敏度的测定中,单位1(江南大学)的抗体IC50的平均值为3.22 μg/L,灵敏度较...     

Modeling of frost growth and frost properties with airflow over a flat plate

A physical model of frost layer growth and frost properties with airflow over a flat plate at subfreezing temperature was developed. Frost roughness was measured, and an empirical correlation for the average frost roughness was suggested. Heat and mass transfer coefficients were calculated using the modified Prandtl mixing-length scheme containing the effects of both frost roughness and turbulent boundary layer thickness. Frost thermal conductivity was theoretically analyzed by solving the combined equations of air equivalent conductivity and thermal conductivity of the frost inner layer. Based on the present model, heat and mass transfer coefficient, frost thermal conductivity, frost thickness, frost mass concentration and frost density with time and space were estimated. The model showed good agreement with the basic trends of the test data taken from other literature. Spatial and temporal changes of heat flux and frost surface temperature were also investigated.     

痘苗病毒滴度测定用国家标准品的制备

目的制备病毒载体疫苗滴度测定用国家标准品。方法以鸡胚成纤维细胞制备痘苗病毒,经密度梯度超速离心纯化后,加入天花疫苗保护剂,分装冻存,分发至我国4个不同实验室,按照统一的结晶紫染色蚀斑法实验方案进行协作标定,每次实验稀释后取2个稀释度,每个稀释度设5个平行孔,计算各实验室检测结果的几何均数,对标准品中痘苗病毒滴度进行赋值。检测病毒标准品于不同温度下保存不同时间的稳定性,并对病毒标准品的各项质量指标进行检定。结果 4个实验室共测定44次,4个实验室间的检测结果差异无统计学意义(P>0.05),各实验室检测结果的几何平均数为3.05×106PFU/ml,95%CI为2.00×106～4.65×106PFU/ml,标准差为0.09,总变异系数1.41%。制备的痘苗病毒标准品于4℃可稳定放置10 d,37℃放置稳定性较差。制备的痘苗病毒标准品装量检查合格(1 ml/支);pH值为6.9;无菌试验合格;分装重量精密性为0.95%。结论制备的痘苗病毒标准品可作为痘病毒载体疫苗滴度测定用标准品。     

苏云金芽胞杆菌不同发酵阶段的补糖发酵

通过苏云金芽胞杆菌工程菌BMB005发酵不同阶段补加葡萄糖发酵试验,研究了补糖对苏云金芽胞杆菌发酵的影响.结果表明:对数生长期补加葡萄糖可以提高生物量,进而使发酵液中杀虫晶体蛋白的质量分数提高33 1%,而稳定期补加葡萄糖则是通过提高菌体合成晶体蛋白的能力而使发酵液中晶体蛋白的质量分数提高18 1%. 30 L发酵罐补糖实验结果表明:在起始碳源质量浓度为3 0 g/dL的情况下,对数生长期补加1 0 g/dL葡萄糖可以将发酵液中杀虫晶体蛋白的质量分数和生物效价分别提高37%和51%;稳定期补加 1 0 g/dL葡萄糖可以将发酵液中杀虫晶体蛋白的质量分数和生物效价分别提高33 7%和75 1%;而且稳定期补加比对数生长期补加葡萄糖更有利于增效因子的合成.     

抗幽门螺杆菌卵黄抗体的制备及其效价的评定

本论文研究了抗幽门螺杆菌蛋黄抗体(IgY—HP)的制备方法。以HP超声粉碎上清和HP尿素酶混合抗原物免疫鸡蛋，从蛋黄中提取纯化得到了很高纯度的IgY—HP。ELISA实验表明，IgY—HP的抗HP效价和一般IgY相比提高了32倍。其可以应用于食品保健或各种HP相关胃病的抗菌免疫治疗中。     

Curcumin inhibits influenza virus infection and haemagglutination activity

Curcumin (diferuloylmethane) is a widely used spice and colouring agent in food. Accumulated evidence indicates that curcumin is associated with a great variety of pharmacological activities, including an anti-microbial effect. In this study, the anti-influenza activity of curcumin was evaluated. Our results demonstrated that treatment with 30 μM curcumin reduced the yield of virus by over 90% in cell culture. The EC₅₀ determined using plaque reduction assays was approximately 0.47 μM (with a selective index of 92.5). Time of drug addition experiments demonstrated curcumin had a direct effect on viral particle infectivity that was reflected by the inhibition of haemagglutination; this effect was observed in H1N1 as well as in H6N1 subtype. In contrast to amantadine, viruses did not develop resistance to curcumin. Furthermore, by comparison of the antiviral activity of structural analogues, the methoxyl groups of curcumin do not play a significant role in the haemagglutinin interaction.     

影响干法腈纶成纤聚合物特性粘度的因素研究

介绍了干法腈纶生产中,影响纺丝原液性能的主要指标是聚合物的特性粘度,考察催化剂、铁离子、pH值、聚合反应温度、烘干条件对特性粘度的影响,提出在生产中控制这些影响因素的对策。     

不同品种马属动物的免疫效果分析

目的分析不同品种马属动物的免疫效果。方法对不同地区、不同品种的马属动物进行分群,采用相同的破伤风类毒素及免疫方法免疫,分析不同群之间的抗毒素效价、免疫成功率、形成免疫耐受的程次、马匹淘汰率的差异及平均免疫寿命。结果不同群之间免疫效果存在差异,身体素质和健康状况不同的马匹,免疫效果不同。结论要生产高效价的免疫血清,应选择高质量的马匹,可采用育种方法培育出适合免疫血清生产用的优良马匹。     

The effect of stationary and sweeping frequency AC electric fields on frost crystal removal on a cold plate

The effect of stationary and sweeping frequency AC electric fields on frost crystals growth and frost control/removal on a cold plate was studied for the first time in this paper. The main results of this study showed that the presence of AC electric fields can greatly affect both the frost crystals growth pattern and mass accumulation on cold surfaces. The ice surface electrical properties and basic electrostatics were used to explain the main findings in this paper. Up to 46% frost reduction was obtained when the electric field frequency spanned 370 Hz to 7.5 kHz while the applied voltage was 14.5 kV. Two different sets of environmental conditions were tested, which showed that the plate temperature placed an important effect on frost crystals growth under electric fields. An optimum application time of the AC electric fields was found based on least frost mass accumulation on the cold plate.