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槟榔炮制阶段优势霉菌分析及其防霉剂的研制 总被引:1,自引:1,他引:1
分别采用营养琼脂培养基、高盐察氏培养基、牛肉膏蛋白胨培养基、高氏1号培养基、马铃薯营养琼脂培养基(加入山梨醇)分析炮制阶段槟榔中微生物菌群的基本构相,其中霉菌为主要菌群,数量为总菌落数的30%左右;进一步对炮制阶段槟榔中的霉菌进行分离纯化,经斜面和湿室栽片培养后,所得青霉菌株数为菌株总数的40%左右,表明青霉为优势霉菌。分别采用钠他霉素、脱氢醋酸钠及其复合防霉剂对青霉进行抑菌活性测定,三者的MIC值分别为10,90,80mg/kg,抑菌圈直径分别为8.4,9.8,9.1nim,同时确定钠他霉素与脱氢醋酸钠以1:500混合时对青霉的防霉效果最佳。 相似文献
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Microbial enhanced oil recovery (MEOR) applies biotechnology to improve residual crude oil production from substratum reservoir. MEOR includes in-situ MEOR and ex-situ MEOR. The former utilizes microbial growth and metabolism in the reservoir, and the latter directly injects desired active products produced by microbes on the surface. Taking biosurfactant-producing strain Pseudomonas aeruginosa WJ-1 for research objects, in-situ enhanced oil recovery and ex-situ enhanced oil recovery by biosurfactant-producing strain WJ-1 were comparatively investigated in sand-pack columns.The results showed that P.aeruginosa WJ-1 really proliferated in sand-pack columns, produced 2.66 g/L of biosurfactant, altered wettability, reduced oil-water interfacial tension (IFT) and emulsified crude oil under simulated in-situ process. Results also showed that higher biosurfactant concentration, lower IFT, smaller average diameters of emulsified crude oil were obtained in in-situ enhanced oil recovery experiment than those in ex-situ enhance oil recovery experiment. Similar wettability alteration was observed in both in-situ and ex-situ enhanced oil recovery experiment. The flooding experiments in sand-pack columns revealed that the recovery of in-situ was 7.46%/7.32% OOIP (original oil in place), and the recovery of the ex-situ was 4.64%/4.49% OOIP. Therefore, in-situ approach showed greater potential in enhancing oil recovery in contrast with ex-situ approach. It is recommended that the stimulation of indigenous microorganisms rather than injection of microbial produced active products should be applied when MEOR technologies were employed. 相似文献
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Abstract In this article, biosurfactant production from low-cost and renewable substrate and its application in petroleum industry were studied. Pseudomonas aeruginosa DG30 grew on used vegetable oil and accumulated 15.56 g/L of biosurfactant at 72 hr. The biosurfactant exhibited excellent surface activity and stability. Sand package tests showed an approximately 20% increase of oil recovery by the treatment of bacterial culture broth. Single-well stimulation tests were conducted in a heavy oil reservoir in Dagang Oilfield, Tianjin, China. The blockages in the wellbore areas were removed effectively and more than 616 tons of crude oil was enhanced. 相似文献
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