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991.
Dr. Veronika Papoušková Dr. Pavel Kadeřávek Olga Otrusinová Alžbeta Rabatinová Dr. Hana Šanderová Jiří Nováček Dr. Libor Krásný Prof. Vladimír Sklenář Dr. Lukáš Žídek 《Chembiochem : a European journal of chemical biology》2013,14(14):1772-1779
The partially disordered δ subunit of RNA polymerase was studied by various NMR techniques. The structure of the well‐folded N‐terminal domain was determined based on inter‐proton distances in NOESY spectra. The obtained structural model was compared to the previously determined structure of a truncated construct (lacking the C‐terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C‐terminal domain. Various 15N relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C‐terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transient contacts of the C‐terminal tail with the N‐terminal domain and with itself were identified. A propensity of the C‐terminal domain to form β‐type structures was obtained by chemical shift analysis. Comparison with the paramagnetic relaxation enhancement indicated a well‐balanced interplay of repulsive and attractive electrostatic interactions governing the conformational behavior of the C‐terminal domain. The results showed that the δ subunit consists of a well‐ordered N‐terminal domain and a flexible C‐terminal domain that exhibits a complex hierarchy of partial ordering. 相似文献
992.
Yasser Kiani 《热应力杂志》2013,36(12):1495-1518
Based on the uncoupled thermoelasticity assumptions, axisymmetric thermally induced vibrations of a circular plate made of functionally graded materials (FGMs) are analyzed. Each thermomechanical property of the circular plate is assumed to be functions of temperature and thickness coordinate. Solution of the transient one-dimensional heat conduction equation with the arbitrary type of time-dependent boundary conditions is carried out employing the central finite difference method combined with the Crank–Nicolson time marching scheme. Afterwards, with the establishment of the associated Hamilton's principle and the accountancy of the von Kármán type of geometrical non-linearity, the motion equations are obtained with the aid of the conventional multi-term Ritz method. The solution of highly coupled non-linear motion equations is obtained utilizing a hybrid iterative Newton–Raphson–Newmark scheme. After validating the developed computer code, some parametric studies are accomplished to show the influences of various involved parameters. It is shown that temperature dependency, geometrical non-linearity, plate thickness, power law index, and the type of thermal in-plane and out-of-plane mechanical boundary conditions, all affect the temporal evolution of plate characteristics. 相似文献
993.
An account is given of an experimental investigation of the normal snarling of highly twisted monofilaments, those used being vulcanized rubber and nylon. An earlier theoretical analysis is corrected, and the experimental results show that, after this correction, the theory put forward for the mechanical properties of the snarling mechanism holds reasonably well for elastic filaments. Although, as would be expected, there are larger deviations from the theory for viscoelastic filaments, the theory still gives a good indication of the behaviour of these filaments under torsion. 相似文献
994.
Graphite/copper composites with high thermal conductivity were fabricated by tungsten addition, which formed a thin tungsten carbide layer at the interface. The microstructure and thermal conductivity of the composite material were studied. The results indicated that the insertion of tungsten carbide layer obviously suppressed spheroidization of copper coating on the graphite particles during the sintering process, and decreased the interfacial thermal resistance of the composites. Compared with the graphite/copper composites without tungsten, the thermal conductivity of the obtained composites was increased by 43.6%. 相似文献
995.
Single-lap shear behaviour of carbon–epoxy composite bolted aircraft fuselage joints at quasi-static and dynamic (5 m/s and 10 m/s) loading speeds is studied experimentally. Single and multi-bolt joints with countersunk fasteners were tested. The initial joint failure mode was bearing, while final failure was either due to fastener pull-through or fastener fracture at a thread. Much less hole bearing damage, and hence energy absorption, occurred when the fastener(s) fractured at a thread, which occurred most frequently in thick joints and in quasi-static tests. Fastener failure thus requires special consideration in designing crashworthy fastened composite structures; if it can be delayed, energy absorption is greater. A correlation between energy absorption in multi-bolt and single-bolt joint tests indicates potential to downsize future test programmes. Tapering a thin fuselage panel layup to a thicker layup at the countersunk hole proved highly effective in achieving satisfactory joint strength and energy absorption. 相似文献
996.
Surface–groundwater (SW–GW) interactions constitute a critical proportion of the surface and groundwater balance especially during dry conditions. Conjunctive management of surface and groundwater requires an explicit account of the exchange flux between surface and groundwater when modelling the two systems. This paper presents a case study in the predominantly gaining Boggabri–Narrabri reach of the Namoi River located in eastern Australia. The first component of the study uses the Upper Namoi numerical groundwater model to demonstrate the importance of incorporating SW–GW interactions into river management models. The second component demonstrates the advantages of incorporating groundwater processes in the Namoi River model.Results of the numerical groundwater modelling component highlighted the contrasting groundwater dynamics close to, and away from the Namoi River where lower declines were noted in a near-field well due to water replenishment sourced from river depletion. The contribution of pumping activities to river depletion was highlighted in the results of the uncertainty analysis, which showed that the SW–GW exchange flux is the most sensitive to pumping rate during dry conditions. The uncertainty analysis also showed that after a drought period, the 95% prediction interval becomes larger than the simulated flux, which implies an increasing probability of losing river conditions. The future prospect of a gaining Boggabri–Narrabri reach turning into losing was confirmed with a hypothetical extended drought scenario during which persistent expansion of groundwater pumping was assumed. The river modelling component showed that accounting for SW–GW interactions improved the predictions of low flows, and resulted in a more realistic calibration of the Namoi River model.Incorporating SW–GW interactions into river models allows explicit representation of groundwater processes that provides a mechanism to account for the impacts of additional aquifer stresses that may be introduced beyond the calibration period of the river model. Conventional river models that neglect the effects of such future stresses suffer from the phenomenon of non-stationarity and hence have inferior low flow predictions past the calibration period of the river model. The collective knowledge acquired from the two modelling exercises conducted in this study leads to a better understanding of SW–GW interactions in the Namoi River thus leading to improved water management especially during low flow conditions. 相似文献
997.
AbstractErosion–corrosion is encountered in a large variety of engineering industries. In such environments, protective coatings are used. In this investigation, erosion–corrosion of the Ni–20Cr coating on nickel and iron based superalloys has been investigated by subjecting them to the boiler of coal fired thermal power plant at the temperature zone of 540°C for 1000 h duration. The erosion–corrosion kinetics of the plasma sprayed Ni–20Cr coating on different superalloys has been investigated. XRD, SEM with EDS and EPMA have been used to analyse the eroded–corroded products along the surface and cross-section. Main phases identified in all the Ni–20Cr coated superalloys after exposure are NiO, Cr2O3, Al2O3 and SiO2. Aluminium has penetrated from the bond coat to the top coat along the splat boundaries. Oxides of chromium, nickel and aluminium are recognized as protective oxides for boiler environment. The probable mechanism of attack for Ni–20Cr coating in the given boiler environment is discussed. 相似文献
998.
Javed Syed Rahmath Ulla Baig Salem Algarni Y.V.V. Satyanarayana Murthy Mohammad Masood Mohammed Inamurrahman 《International Journal of Hydrogen Energy》2017,42(21):14750-14774
The rapid growth of vehicular pollution; mostly running on the diesel engine, emissions emerging are the concerns of the day. Owing to clean burn characteristics features, Hydrogen (H2) as a fuel is the paradigm of the researcher. Extensive research presented in the literature on H2 dual fueled diesel engine reveals, the significant role of H2 in reducing emissions and enhancing the performance of a dual fueled diesel engine. With meager qualitative experiment data, the feasibility to develop an efficient Artificial Neural Network (ANN) model is investigated, the developed model can be utilized as a tool to investigate the H2 dual fueled diesel engine further. In the process of developing an ANN model, engine load and H2 flow rate are varied to register performance and emission characteristics. The creditability of the experiment is ascertained with uncertainty analysis of measurable and computed parameters. Leave-out-one method is adopted with 16 data sets; seven training algorithms are explored with eight transfer function combinations to evolve a competent ANN model. The efficacy of the developed model is adjudged with standard benchmark statistic indices. ANN model trained with Broyden, Fletcher, Goldfarb, & Shanno (BFGS) quasi-Newton backpropagation (trainbfg) stand out the best among other algorithms with regression coefficient ranging between 0.9869 and 0.9996. 相似文献
999.
Magnesium is one of the lightest structural metals that has been used in different industries such as automobile, aerospace and electronics. However, in fusion joining of magnesium alloys, porosity is one of the main drawbacks to achieve a weld with desirable properties. The oxide layer existing on the surface of magnesium alloy is one of the causes of pore formation in the weld bead. In the current study, a fiber laser with a power of up to 4 kW is used to weld samples in a zero-gap lap joint configuration. Two groups of samples are studied: as-received (AR) surfaces (where an oxide layer remains on the surface) and treated surfaces. The surface treatment includes two techniques: mechanically removed (MR) and the use of a plasma arc (PA) as a preheating source. Also, a separate set of experiments are designed for preheating samples in a furnace for comparison with the PA-treated results. To reveal the chemical compositions of the welds and metal sheet surfaces, an energy dispersive spectroscopy (EDS) is performed. Surface chemical compositions are tested by X-ray photoelectron spectroscopy-reflected electron energy loss spectroscopy (XPS-REELS) to characterize the surface composition on AR and PA-treated samples. The dynamic behavior of the weld pool and laser-induced plasma plume is monitored in real-time using a high speed CCD camera to investigate the stability of the laser welding process. The presence of the oxide layer at the faying surface of two overlapped sheets results in an unstable process. The obtained results reveal that the preheating procedure can effectively mitigate pore formation at the interface of the two overlapped sheets. 相似文献
1000.
Thermal conductivity of SiCp/Cu composites was usually far below the expectation, which is usually attributed to the low real thermal conductivity of matrix. In the present work, highly pure Cu matrix composites reinforced with acid washed SiC particles were prepared by the pressure infiltration method. The interfacial microstructure of SiCp/Cu composites was characterized by layered interfacial products, including un-reacted SiC particles, a Cu–Si layer, a polycrystalline C layer and Cu–Si matrix. However, no Cu3Si was found in the present work, which is evidence for the hypothesis that the formation of Cu3Si phase in SiC/Cu system might be related to the alloying elements in Cu matrix and residual Si in SiC particles. The thermal conductivity of SiCp/Cu composites was slightly increased with the particle size from 69.9 to 78.6 W/(m K). Due to high density defects, the real thermal conductivity of Cu matrix calculated by H–J model was only about 70 W/(m K). The significant decrease in thermal conductivity of Cu matrix is an important factor for the low thermal conductivity of SiCp/Cu composites. However, even considered the significant decrease of thermal conductivity of Cu matrix, theoretical values of SiCp/Cu composites calculated by H–J model were still higher than the experimental results. Therefore, an ideal particle was introduced in the present work to evaluate the effect of interfacial thermal resistance. The reverse-deduced effective thermal conductivities of ideal particles according to H–J model was about 80 W/(m K). Therefore, severe interfacial reaction in SiCp/Cu composites also leads to the low thermal conductivity of SiCp/Cu composites. 相似文献