微波辅助提取葛根黄酮的工艺研究

以葛根为原料,乙醇为溶剂,采用微波辅助提取葛根总黄酮。考察了溶剂体积分数、固液比、提取时间、微波功率以及提取次数对提取液葛根总黄酮含量的影响。并在单因素试验的基础上,进行4因素3水平的中心组合试验,通过响应面分析优化确定葛根总黄酮的提取工艺。结果表明,在乙醇体积分数56%、固液比1∶25、提取时间31s、微波功率539W、提取次数为2次的条件下总黄酮含量最高,模型预测值可达7.26mg/g,实际验证值为7.32mg/g。     

栀子根挥发油的成分分析

分别采用超临界CO2萃取法和水蒸气蒸馏法提取栀子根的挥发油.并通过气相色谱-质谱联用技术对其进行分析,以峰面积归一化法对各组分进行定量计算.结果表明,超临界CO2萃取法提取物和水蒸气蒸馏法提取物中分别有65种物质和59种物质被鉴别,占总挥发油的86.546％和73.626％.     

当归红芪超滤物对miR-21-5P靶向调控TGF-β1介导放射性心肌纤维化的干预效应

目的：揭示miR-21-5P靶向调控TGF-β1是介导心肌成纤维细胞活化的关键机制,明确当归红芪超滤物对miR-21-5P靶向调控TGF-β1机制的干预效应。方法：（1）将细胞随机分为正常组、辐照组,辐照组接受6Gry单次照射,后采用RT-PCR检测miR-21-5P,Western Blot检测α-SMA、TGF-β1的表达;（2）细胞随机分为正常组、辐照组、miR-21-5PNC+辐照组、miR-21-5Pinhibitor+辐照组,后采用RT-PCR检测miR-21-5P,Western Blot检测TGF-β1、Smad3、Smad7的表达,免疫荧光染色检测MMP-9、TIMP1表达,EDU染色检测细胞凋亡;（3）将细胞随机分为正常组、辐照组、RAS-RH+辐照组,后采用RT-PCR检测miR-21-5P,Western Blot 检测TGF-β1、Smad3、Smad7的表达,免疫荧光染色检测MMP-9、TIMP1表达,EDU染色检测细胞增殖。结果：（1）辐照组miR-21-5P、α-SMA、TGF-β1的表达明显高于正常组（P<0.01）,表明X线辐射可诱导心肌成纤维细胞活化,同时miR-21-5P、TGF-β1与心肌纤维化的发生密切相关;（2）miR-21-5Pinhibitor+辐照组中miR-21-5P、TGF-β1、Smad3、TIMP1表达明显低于辐照组、miR-21-5PNC+辐照组,MMP-9、Smad7表达明显高于辐照组,EDU染色阳性率降低、细胞数目减少,表明miR-21-5P靶向调控TGF-β1是介导心肌纤维化的关键机制;（3）RAS-RH+辐照组miR-21-5P、α-SMA、TGF-β1、Smad3、TIMP1表达明显低于辐照组,MMP-9、Smad7表达明显高于辐照组,EDU染色阳性率、细胞数目略有降低,表明RAS-RH可通过抑制miR-21-5P靶向调控TGF-β1机制干预放射性心肌纤维化的发生。结论：RAS-RH可通过抑制miR-21-5P靶向调控TGF-β1的机制干预心肌纤维化的发生。     

中药红景天提取物抗氧化活性的研究

用乙醇提取中药红景天的抗氧化物质,并采用羟基自由基体系和超氧阴离子自由基体系对中药红景天提取液的抗氧化性进行研究,并同特丁基对苯二酚(TBHQ)、维生素E进行比较。结果表明,红景天提取液在两种体系中都具有很好的抗氧化能力,且清除超氧阴离子自由基的能力要好于羟基自由基,并大于维生素E。     

HPLC-ESI-MS/MS分析黄芪蜜炙前后活性成分差异

建立了HPLC-ESI-MS/MS联用技术分析生黄芪与炙黄芪的成分,并比较两者成分差异。采用Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm×5 μm）,以0.1%甲酸乙腈溶液-0.1%甲酸水溶液为流动相进行梯度洗脱,流速1 mL/min,检测波长203 nm,柱温25 ℃;使用ESI离子源,分别在正、负离子模式下进行全扫描和二级扫描,质量扫描范围m/z 100~2000。采用该方法鉴定了生黄芪与炙黄芪中的62种成分,并对黄芪蜜炙前后的成分进行了比较,实验结果可为黄芪炮制方法的选择提供参考依据。     

Radix Tetrastigma extract from different origins protect RAW264.7 macrophages against LPS-induced inflammation

Radix Tetrastigma (RT) is a medicinal plant and functional food in China, distributed in various places in the south of China. Radix Tetrastigma extract (RTE) from different origins were collected and analyzed for their anti-inflammatory effects. Different RTEs showed different abilities to suppress shape deformation, decrease the nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in RAW 264.7 cells. Subsequently, their bioactive components were compared, and results showed RTEs are rich in flavonoid (85.25-436.70 mg RE/g DW), polysaccharides (100.45-349.26 mg glucose/g DW), phenolic (12.92-225.40 mg GAE/g DW) and protein contents (4.429-7.719 mg/g DW). Principal component analysis (PCA) and correlation studies indicated that anti-inflammatory capacity could be more associated with total flavonoid contents. High-performance liquid chromatography (HPLC) and Ultraperformance liquid chromatography-time-of flight mass spectrometry (UPLC-TOF/MS) analysis were conducted, and results showed that rutin, isoquercitrin, kaempferol-3-O-rutinoside and astragalin were main flavonoid compounds, among them astragalin exhibited a prior protective effect, suggesting it might be responsible for RTE's excellent anti-inflammatory capacity.     

Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii

Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedur...     

Comparison of techniques for extraction of isoflavones from the root of Radix Puerariae: Ultrasonic and pressurized solvent extractions

Radix Puerariae (RP), a traditional Chinese medicinal herb, has been used for a variety of disease prevention and treatment purposes. Three isoflavones, puerarin, daidzin and daidzein, isolated from RP are responsible for its broad therapeutic effects. In the present study, we demonstrate the application of three extraction methods, traditional, pressurized solvent extraction (PSE) and ultrasonic techniques, for preparing ethanolic RP extract. A comparison of the three extraction methods showed preferential higher yields of the three major isoflavones when the ultrasonic technique was applied. The extraction yield became higher as the mean size of RP particles decreased while the total accumulated power varied from 20 to 80 MJ at the solid-to-solvent ratios of 1:5 and 1:10 (g/ml). The relationship among accumulated energies, extraction yields and mean particle sizes under different extraction ratios using ultrasound was also discussed. This study proves that using the ultrasonic method should be the most economic way for enhancing the extraction yield of isoflavones-containing herbal extract in a short time with a reduced amount of solvent at a lower temperature.     

麦冬药渣中麦冬多糖的提取工艺研究

目的探索并确定从麦冬药渣中提取麦冬多糖的最佳工艺,为麦冬药渣的综合利用提供依据。方法以多糖得率为指标,通过单因素试验研究4个提取因素:提取温度、提取时间、料液比、提取次数对麦冬药渣中多糖提取效率的影响,然后,通过正交试验对提取工艺条件进行优化。结果优选出麦冬多糖的最佳提取条件为:提取温度:100℃,料液比1∶12,提取时间为120 min,提取2次;另外,与直接从生药中提取麦冬多糖相比,利用药渣提取麦冬多糖,虽多糖得率有所下降,但多糖含量却有提高。结论该工艺条件可重复,多糖得率较高,此方法使麦冬中的有效成分综合利用,减少了中药资源的浪费。