全文获取类型
收费全文 | 276篇 |
免费 | 36篇 |
国内免费 | 6篇 |
专业分类
电工技术 | 1篇 |
综合类 | 7篇 |
化学工业 | 150篇 |
金属工艺 | 1篇 |
机械仪表 | 2篇 |
建筑科学 | 3篇 |
矿业工程 | 1篇 |
能源动力 | 1篇 |
轻工业 | 142篇 |
水利工程 | 1篇 |
一般工业技术 | 4篇 |
原子能技术 | 3篇 |
自动化技术 | 2篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2022年 | 14篇 |
2021年 | 12篇 |
2020年 | 5篇 |
2019年 | 8篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 13篇 |
2015年 | 17篇 |
2014年 | 17篇 |
2013年 | 25篇 |
2012年 | 29篇 |
2011年 | 15篇 |
2010年 | 12篇 |
2009年 | 16篇 |
2008年 | 21篇 |
2007年 | 18篇 |
2006年 | 9篇 |
2005年 | 12篇 |
2004年 | 15篇 |
2003年 | 9篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
排序方式: 共有318条查询结果,搜索用时 15 毫秒
71.
N. P. Ghildyal M. S. Thakur S. Srikanta S. A. Jaleel S. G. Prapulla M. S. Prasad P. N. Devi B. K. Lonsane 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1987,38(4):221-234
Studies on the rheology of Streptomyces fradiae ScF-5 in a 10-dm3 submerged fermenter for the production of intracellular glucose isomerase revealed that the apparent viscosity of the broth increased with increase in cell density up to 24 h and then declined while the density of the broth remained constant. The plot of shear stress versus shear rate at various fermentation periods showed the applicability of the Casson model. The yield stress and consistency index were found to increase gradually then decrease subsequently. In contrast, the flow behaviour index was less than unity and remained constant throughout the fermentation. The results contrast with the findings of other workers on the Newtonian and non-Newtonian character of fermentation broths and the applicability of the power law model to such systems. 相似文献
72.
Dr. Andrew P. Marshall Prof. Erin E. Carlson 《Chembiochem : a European journal of chemical biology》2023,24(7):e202200757
Streptomyces coelicolor is a prolific producer of natural products and serves as a model organism for their study. It produces several pigmented antibiotics, the best-studied of which are the actinorhodins. We used a combination of liquid chromatography-mass spectrometry (LC–MS) and computational tools used for annotating the detected species (e. g., spectral matching, in-silico predictors, molecular networking) to identify putative new actinorhodin analogs. These studies led to the discovery of the first trimeric benzoisochromanequinone, θ-actinorhodin ( 1 ). Further metabolomics analysis revealed that the relative amounts of shunt products produced were similar between the two growth conditions explored. This suggests that, while substantially different products were being produced, the biosynthetic gene clusters were similarly active. Overall, this work describes the discovery of the first trimeric benzoisochromanequinone and explores the biosynthetic processes that might lead to its production by metabolomics analysis of relevant intermediates. 相似文献
73.
Vaishnavi Manikkasundaram Abirami Baskaran Manigundan Kaari Vignesh Angamuthu Gopikrishnan Venugopal Radhakrishnan Manikkam 《Journal of surfactants and detergents》2023,26(4):491-503
The aim of this investigation is to produce and characterize biosurfactant from Streptomyces sp. HRB1 and to evaluate its biomedical and bioremediation potential. Biosurfactant producing property of Streptomyces sp. HRB1 isolated from petroleum contaminated soil was confirmed by hemolytic and oil spread assays. Based on the results of FT-IR spectral and GC–MS analysis, the biosurfactant was confirmed as glycolipid type. Biosurfactant from Streptomyces sp. HRB1 exhibited 71% inhibition against Pseudomonas aeruginosa biofilm formation, 77.33% quorum sensing inhibition property against Chromobacterium violeceum MTCC 2656, more than 80% inhibition in antioxidant assays namely, DPPH, ABTS, and metal chelation, promising anti-proliferative activity against leukemia and myeloma cells with low IC50 values, 96% decolorization of malachite green within 48 h of reaction time, and minimal toxicity against normal cell lines in dose-dependent manner. The taxonomic position of the potential strain HRB1 was further confirmed as Streptomyces enissocaesilis HRB1 based on their phenotypic and molecular characteristics. To conclude, Streptomyces enissocaesilis HRB1 isolated from petroleum-contaminated soil is a promising source for low-cost production of glycolipid biosurfactant with potential biomedical and environmental applications such as antiphytofungal, antibiofilm, anti-quorum sensing, antioxidant, anticancer, and dye degradation properties. 相似文献
74.
75.
根据S.pristinaespiralis E-71普那霉素发酵过程的代谢特性提出了一个针对产物合成期的普那霉素合成动力学方程,结合液膜-孔扩散理论,建立了描述2.5L通气搅拌发酵罐中JD-1大孔吸附树脂吸附分离原位耦合发酵过程的动力学模型,并获得产物生成速率常数、产物抑制常数及液膜传质和孔内扩散系数。结果表明该发酵-吸附耦合过程动力学模型能较好地描述这一原位分离耦合发酵生产普纳霉素过程。在此基础上,考察了树脂添加量及树脂半径对原位分离耦合发酵过程的影响,结果表明添加JD-1树脂能有效移走发酵液主体相中高达90%的普那霉素,且原位分离效果随树脂粒径减小而增强,在一定范围内随树脂添加量的增加而增强,当添加70g.L-1半径为0.195mm的树脂时,普纳霉素产量达到1.01g.L-1,其中被树脂吸附的高于95%。 相似文献
76.
The effects of nitrogen sources on streptolydigin production and distribution of secondary metabolites were investigated for flask cultured S.lydicus AS 4.2501.When peptone,asparamide,and glutamic acid were ex- amined as the nitrogen source,respectively,liquid chromatography-mass spectrometry(LC-MS)and photodiode array(PDA)analyses revealed the formation of two analogues of streptolydigin in the fermentation broth.When soybean meal was used as the source of nitrogen,three analogues of streptolydigin were detected.The use of am- monium sulfate as a source of nitrogen resulted in a lower pH value of the fermentation system,thus inhibiting streptolydigin biosynthesis and changing the metabolic profiling.Among the nitrogen sources that were made use of,glutamic acid was most favorable to the formation of streptolydigin.Simultaneously,this study also showed that the changing nitrogen sources resulted in altering the production and relative ratios of streptolydigin and its analogues. 相似文献
77.
Won-Bok Chae Young-Bum Kim Sung-Won Choi Hyang-Bok Lee Eun-Ki Kim 《Korean Journal of Chemical Engineering》2009,26(2):438-443
To be an effective microbial biocide, Streptomyces kasugaensis should be converted into spore during cultivation process for successful long-term storage. By statistical design methods,
culture conditions including medium components and operating parameters were optimized and more than 100 times increase in
spore yield was achieved. Addition of spent culture fluid (100 ppm), EDTA (30 ppm), mycophenolic acid (32 ppm) with combination
of pH up-shock (5.5 to 8.5) increased total viable cell and spore conversion rate, resulting in 1.6×107 (spore/mL) in 5 days of culture in a fermenter. This result provides a practical method for obtaining high spore number for
commercial production of Streptomyces kasugaensis as a microbial pesticide. 相似文献
78.
Cao Van Anh Joo-Hee Kwon Jong Soon Kang Hwa-Sun Lee Chang-Su Heo Hee Jae Shin 《International journal of molecular sciences》2022,23(22)
Chemical investigation of the ethyl acetate extract from the culture broth of the marine-derived actinobacterium Streptomyces ardesiacus 156VN-095 led to the isolation of three hitherto undescribed angucycline glycosides, including urdamycins W and X (1 and 2) and grincamycin U (9), as well as their seven known congeners. The structures of the new compounds were elucidated by means of spectroscopic methods (HRESIMS, 1D and 2 D NMR) and comparison of their experimental data with literature values. Compounds 1–3 and 9 were evaluated for their anti-Gram-positive bacterial effect and cytotoxicity against six cancer cell lines. Compound 1 displayed significant cytotoxicity against all the tested cell lines with GI50 values of 0.019–0.104 µM. Collectively, these findings highlight the potential of angucycline glycosides as leading structures for the development of new anti-cancer drugs. 相似文献
79.
80.