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121.
Elisabetta Tabolacci Veronica Nobile Cecilia Pucci Pietro Chiurazzi 《International journal of molecular sciences》2022,23(10)
A dynamic mutation in exon 1 of the FMR1 gene causes Fragile X-related Disorders (FXDs), due to the expansion of an unstable CGG repeat sequence. Based on the CGG sequence size, two types of FMR1 alleles are possible: “premutation” (PM, with 56-200 CGGs) and “full mutation” (FM, with >200 triplets). Premutated females are at risk of transmitting a FM allele that, when methylated, epigenetically silences FMR1 and causes Fragile X syndrome (FXS), a very common form of inherited intellectual disability (ID). Expansions events of the CGG sequence are predominant over contractions and are responsible for meiotic and mitotic instability. The CGG repeat usually includes one or more AGG interspersed triplets that influence allele stability and the risk of transmitting FM to children through maternal meiosis. A unique mechanism responsible for repeat instability has not been identified, but several processes are under investigations using cellular and animal models. The formation of unusual secondary DNA structures at the expanded repeats are likely to occur and contribute to the CGG expansion. This review will focus on the current knowledge about CGG repeat instability addressing the CGG sequence expands. 相似文献
122.
In 2020, gastric cancer was the fourth leading cause of cancer deaths globally. About 90% of gastric cancers are sporadic and the vast majority are correlated with Helicobacter pylori infection; whereas familial clustering is observed in about 10% of cases. Gastric cancer is now considered to be a disease originating from dysregulated self-renewal of the gastric glands in the setting of an inflammatory environment. The human stomach contains two types of gastric units, which show bi-directional self-renewal from a complex variety of stem cells. This review focuses on recent progress concerning the characterization of the different stem cell populations and the mainly mesenchymal signals triggering their stepwise differentiation as well as the genesis of pre-cancerous lesions and carcinogenesis. Furthermore, a model is presented (Lectin-triggered Receptor Blocking Hypothesis) explaining the role of the lectin TFF1 as an antral tumor suppressor possibly regulating Lgr5+ antral stem cells in a paracrine or maybe autocrine fashion, with neighboring antral gland cells having a role as niche cells. 相似文献
123.
Matteo Sibilano Valentina Tullio Gaspare Adorno Isabella Savini Valeria Gasperi Maria Valeria Catani 《International journal of molecular sciences》2022,23(10)
Among the surrounding cells influencing tumor biology, platelets are recognized as novel players as they release microvesicles (MVs) that, once delivered to cancer cells, modulate signaling pathways related to cell growth and dissemination. We have previously shown that physiological delivery of platelet MVs enriched in miR-126 exerted anti-tumor effects in different breast cancer (BC) cell lines. Here, we seek further insight by identifying AKT2 kinase as a novel miR-126-3p direct target, as assessed by bioinformatic analysis and validated by luciferase assay. Both ectopic expression and platelet MV-mediated delivery of miR-126-3p downregulated AKT2 expression, thus suppressing proliferating and invading properties, in either triple negative (BT549 cells) or less aggressive Luminal A (MCF-7 cells) BC subtypes. Accordingly, as shown by bioinformatic analysis, both high miR-126 and low AKT2 levels were associated with favorable long-term prognosis in BC patients. Our results, together with the literature data, indicate that miR-126-3p exerts suppressor activity by specifically targeting components of the PIK3/AKT signaling cascade. Therefore, management of platelet-derived MV production and selective delivery of miR-126-3p to tumor cells may represent a useful tool in multimodal therapeutic approaches in BC patients. 相似文献
124.
Shafiq Murad Susanne Michen Alexander Becker Monika Füssel Gabriele Schackert Torsten Tonn Frank Momburg Achim Temme 《International journal of molecular sciences》2022,23(10)
In glioblastoma, non-classical human leucocyte antigen E (HLA-E) and HLA-G are frequently overexpressed. HLA-E loaded with peptides derived from HLA class I and from HLA-G contributes to inhibition of natural killer (NK) cells with expression of the inhibitory receptor CD94/NKG2A. We investigated whether NK cells expressing the activating CD94/NKG2C receptor counterpart were able to exert anti-glioma effects. NKG2C+ subsets were preferentially expanded by a feeder cell line engineered to express an artificial disulfide-stabilized trimeric HLA-E ligand (HLA-E*spG). NK cells expanded by a feeder cell line, which facilitates outgrowth of conventional NKG2A+, and fresh NK cells, were included for comparison. Expansion via the HLA-E*spG feeder cells selectively increased the fraction of NKG2C+ NK cells, which displayed a higher frequency of KIR2DL2/L3/S2 and CD16 when compared to expanded NKG2A+ NK cells. NKG2C+ NK cells exhibited increased cytotoxicity against K562 and KIR:HLA-matched and -mismatched primary glioblastoma multiforme (GBM) cells when compared to NKG2A+ NK cells and corresponding fresh NK cells. Cytotoxic responses of NKG2C+ NK cells were even more pronounced when utilizing target cells engineered with HLA-E*spG. These findings support the notion that NKG2C+ NK cells have potential therapeutic value for treating gliomas. 相似文献
125.
提出了一种串联机器人的改进控制算法。采用一自适应模糊控制器,根据滑模到达条件对滑模切换增益进行估算,消除滑模控制中输出力矩的抖振现象,增强其对不确定性因素的适应能力。采用另一自适应模糊控制器对指数趋近律系数进行修正,改善由于大范围初始位姿产生的偏差而引起的大力矩和速度跳变问题。该方法无需确定被控对象的具体数学模型,具有强鲁棒性和高跟踪精度。基于Lyapunov方法进行了稳定性证明,保证控制系统的稳定性与收敛性。实验结果表明,该方法应用于串联机器人,跟踪效果良好并产生了平滑的力矩输出和速度输出。 相似文献
126.
基于掩模金字塔的高精度全局运动估计算法 总被引:1,自引:0,他引:1
在视频序列的全局运动估计中,前景运动对象的存在常常会大幅度地降低估计的精度,为此提出一种对前景对象自适应的高精度全局运动估计算法.该算法以像素块为单位,利用块内外点的比重判定前景区域,同时引入马尔可夫聚类方法进行后处理,有效地提高了运动对象的定位精度;通过对目标函数引入权重系数增强对残差的鲁棒性,以进一步提高算法的估计精度.此外,基于像素掩模的3层金字塔构建序列图像,并将改进的梯度方法引入到优化过程中,提高了算法的实时性.对不同运动类型的标准视频序列的实验结果表明,该算法有效地提高了全局运动估计的精度和速度. 相似文献
127.
提出了基于BP神经网络的车牌自动定位方法。实验表明,由此建立的车牌自动定位模型适角于各类机动车辆,总定位率为99.5%,达到了业务使用的要求。 相似文献
128.
Ammad Ahmad Farooqi Gulnara Kapanova Sundetgali Kalmakhanov Gulnur Tanbayeva Kairat S. Zhakipbekov Venera S. Rakhmetova Marat K. Syzdykbayev 《International journal of molecular sciences》2022,23(15)
Landmark discoveries in molecular oncology have provided a wide-angle overview of the heterogenous and therapeutically challenging nature of cancer. The power of modern ‘omics’ technologies has enabled researchers to deeply and comprehensively characterize molecular mechanisms underlying cellular functions. Interestingly, high-throughput technologies have opened new horizons for the design and scientific fool-proof evaluation of the pharmacological properties of targeted chemical compounds to tactfully control the activities of the oncogenic protein networks. Groundbreaking discoveries have galvanized the expansion of the repertoire of available pharmacopoeia to therapeutically target a myriad of deregulated oncogenic pathways. Natural product research has undergone substantial broadening, and many of the drugs which constitute the backbone of modern pharmaceuticals have been derived from the natural cornucopia. Baicalein has gradually gained attention because of its unique ability to target different oncogenic signal transduction cascades in various cancers. We have partitioned this review into different sub-sections to provide a broader snapshot of the oncogenic pathways regulated by baicalein. In this review, we summarize baicalein-mediated targeting of WNT/β-catenin, AKT/mTOR, JAK/STAT, MAPK, and NOTCH pathways. We also critically analyze how baicalein regulates non-coding RNAs (microRNAs and long non-coding RNAs) in different cancers. Finally, we conceptually interpret baicalein-mediated inhibition of primary and secondary growths in xenografted mice. 相似文献
129.
130.