食物对两种桂利嗪胶囊人体生物利用度的影响

目的 比较食物对两种桂利嗪胶囊(A 和 B)人体生物利用度的影响。方法 用 HPLC 紫外检测法测 定健康受试者分别在禁食及餐后一次口服 50mg 桂利嗪胶囊 A 或 B 后血清药物浓度。结果 胶囊 A 的 C_max 及 AUC 不受食物影响, 胶囊 B 的吸收受食物影响, 禁食后的 C_max 和 AUC 显著低于餐后的 C_max 和AUC。结论 胶囊 A 的制剂优于胶囊 B 的制剂, 其人体生物利用度不受食物影响。     

多肽螯合钙的研究进展

钙作为一种必需营养素,在人体中有着极为重要的作用。现如今,补钙制剂在市场上占有极大的份额,但以无机钙和有机钙为主的第一、第二代补钙制剂仍有较大缺陷,吸收率和生物利用率低,而多肽螯合钙作为第三代具有活性结构的生物钙补钙制剂稳定性好、抗干扰能力强、吸收效果好、生物利用度高,因此越来越受到关注。该文对多肽螯合钙的结合机制、制备方法、吸收途径、生物利用度等方面的研究进行综述,以期对补钙制剂的研究及开发提供新思路。     

类蛋白反应修饰的牡蛎肽锌结合物的生物利用性

通过类蛋白反应(Plastein)修饰制备具有较高锌离子结合能力的牡蛎源肽(EVPPEEH),其锌结合能力为161 mg/g。以缺锌型SD大鼠为试验模型,以牡蛎酶解肽-锌结合物OPZ、类蛋白反应修饰的牡蛎酶解肽-锌结合物LPZ和ZnSO4(ZS)为对照,研究牡蛎源肽(EVPPEEH)与锌的结合物(MZ)在体内的生物利用率和生物利用途径。结果表明:MZ组大鼠血清锌水平,股骨锌储备量及肝脏锌含量和肾脏锌含量均显著高于各对照组。脾脏锌含量MZ组和LPZ组恢复程度相似,均显著高于OPZ组和ZS组。MZ组和各对照组大鼠体重和摄食量均显著增加,效果相似。大鼠小肠转锌蛋白(ZnT1)的表达量MZ组与无机锌组差异不是很明显,而小肽转运蛋白(PepT1)的表达量MZ组最高,相对于ZS组上调了20%,显著高于各对照组,说明大鼠小肠对MZ的吸收利用一部分是通过传统锌离子途径,另一部分是通过小肽途径。MZ有望成为一种新型高效的补锌制剂。     

食品体系组成及加工方式对植物次生代谢物生物利用度的影响研究进展

食用富含植物次生代谢物的食物能够调控人体健康,然而由于溶解性差、化学不稳定等因素,这些次生代谢物的生物利用度通常较低.食品加工导致的食品组成破坏是植物次生代谢物释放的重要条件,同时食品体系组成还会影响植物次生代谢物的吸收.本文综述了脂质和蛋白质等不同食品体系组成以及热处理、机械加工、发酵等食品加工方式对植物次生代谢物生...     

Omega-3 fatty acids – A review of existing and innovative delivery methods

Omega-3 fatty acids are generally under-consumed in Western diets; a factor that may largely be attributed to low intake of oily fish. Although supplementation strategies offer one approach in terms of improving blood fatty acid levels, rates of compliance are generally low due to difficulties in swallowing capsules, or unfavorable aftertastes. Consequently, new approaches, including food-based strategies, may be an alternative approach to improving omega-3 status and the health of public sectors. This paper sets out to discuss and review how the use of novel food vehicle and delivery advancements may be used to improve omega-3 status, which may have wider benefits for public health and well-being.     

提高蛋白多肽口服生物利用度的方法

综述了常用的提高蛋白多肽生物利用度的策略,包括化学修饰、吸收促进剂、粘膜粘附系统、脂质体和纳微球等,系统分析了各种策略的研究现状及优缺点,重点分析了纳微球的研究现状. 指出在纳微球的基础上对影响药物释放和吸收的各方面因素进行系统化、个性化设计,有望设计出可应用于临床的蛋白多肽口服给药系统.     

Improvement of the oral bioavailability of coenzyme Q10 by emulsification with fats and emulsifiers used in the food industry

Coenzyme Q₁₀ (CoQ₁₀) was emulsified with fats and emulsifiers commonly used in the food industry, and the oral bioavailability of the emulsified product was compared with that of a standard commercial product. Five commonly used fats (olive oil, safflower oil, coconut oil, butter, and cocoa butter), four types of emulsifier (lecithin, monoglycerides, calcium stearoyl-2-lactate (CSL), and diacetyl tartaric acid esters of monoglycerides), and two types of aqueous phase (distilled water with or without 8 g/100 g w/w skim milk) were employed in this study. It was found that CoQ₁₀ was more soluble in coconut and safflower oils than in the other fats. In addition, it was demonstrated that emulsion with coconut oil, skim milk aqueous solution, and CSL produced an optimal formulation. Based on the results, a model emulsified CoQ₁₀ product was prepared. Its oral bioavailability was confirmed to be slightly greater than that of a standard commercial CoQ₁₀ product.     

Absorption of anthocyanins through intestinal epithelial cells – Putative involvement of GLUT2

Anthocyanins bioavailability is a major issue regarding their biological effects and remains unclear due to few data available on this matter. This work aimed to evaluate the absorption of anthocyanins at the intestine using Caco‐2 cells. Anthocyanin extract, rich in malvidin‐3‐glucoside, was obtained from red grape skins and tested on Caco‐2 cells. The absorption of anthocyanins, in absence or presence of 1% ethanol, was detected by HPLC/DAD/LC‐MS. Our results showed that this transport was significantly increased in the presence of ethanol especially after 60 min of incubation. In addition, cells that were pretreated for 96 h with anthocyanins (200 μg/mL) showed an increase of their own transport (about 50% increase). Expression of glucose transporters sodium‐dependent glucose transporter 1, facilitative glucose transporters 5, and facilitative glucose transporters 2 was assessed by RT‐PCR. It was found that facilitative glucose transporters 2 expression was increased (60%) in Caco‐2 cells pretreated with anthocyanins, by comparison with controls. When the effect of anthocyanin extract on ³H‐2‐deoxy‐D ‐glucose uptake was tested, an inhibitory effect was observed (about 60% decrease). However, the malvidin aglycone was tested and had no effect. In conclusion, anthocyanins could be absorbed through Caco‐2 cells, and can interfere with their own transport and also with glucose intestinal uptake.