Detailed Structure–Function Correlations of Bacillus subtilis Acetolactate Synthase

Isobutanol is deemed to be a next‐generation biofuel and a renewable platform chemical. 1 Non‐natural biosynthetic pathways for isobutanol production have been implemented in cell‐based and in vitro systems with Bacillus subtilis acetolactate synthase (AlsS) as key biocatalyst. 2 – 6 AlsS catalyzes the condensation of two pyruvate molecules to acetolactate with thiamine diphosphate and Mg²⁺ as cofactors. AlsS also catalyzes the conversion of 2‐ketoisovalerate into isobutyraldehyde, the immediate precursor of isobutanol. Our phylogenetic analysis suggests that the ALS enzyme family forms a distinct subgroup of ThDP‐dependent enzymes. To unravel catalytically relevant structure‐function relationships, we solved the AlsS crystal structure at 2.3 Å in the presence of ThDP, Mg²⁺ and in a transition state with a 2‐lactyl moiety bound to ThDP. We supplemented our structural data by point mutations in the active site to identify catalytically important residues.     

利用生物技术创造甜菜四倍体材料的研究

试验采用甜菜未授粉胚珠培养技术创造纯合二倍体品系，诱变筛选出四倍体高产品系3个，高糖品系6个，高产兼高糖品系3个，抗褐班病性均优于对照品种。     

光学相干层析成像技术确定组织光学特性

光学相干层析成像(OCT)是近年来发展较快的一种新型成像技术,能对生物组织内部的微观结构进行高分辨率的横断面层析成像,具有快速、非侵入及高分辨率等特点,在体生物组织微观结构分析和疾病诊断等方面具有重要的应用价值.综述了采用光学相干层析成像技术确定生物组织光学特性的研究.     

黄曲霉毒素去毒方法研究进展

黄曲霉毒素毒性极强,具有致癌、致畸和致突变作用,在食物链内污染范围广,严重威胁到人类的健康和饮食安全.目前对黄曲霉毒素去毒方法研究较多,其中安全有效的生物学去毒方法具有很好的发展前景.发展安全、高效且环保的黄曲霉毒素去毒方法是今后研究的热点.     

眼球系统中关键屈光元件的光学模型研究

角膜和晶状体是人眼光学系统中两个关键的屈光元件,为了理解眼内各介质和整个眼球的屈光状态以及视网膜上的成像,有利于眼科临床方面的应用,需要分别对二者进行光学特性模型的分析与研究。根据角膜和晶状体的光学特性,应用光学设计软件Zemax和有关的数学工具,从光学成像角度,分别对角膜和晶状体模型进行研究:基于结合人眼光学模型的角膜双二次曲面模型的建立方法,统计分析了我国人眼实测角膜参数的数据;通过对晶状体折射率分布特点的分析,分别在轴向和径向上进行了综合分析。最后给出了符合我国人眼特点的角膜面型的统计数值,完善了我国人眼角膜光学模型的建立;获得了形式简单且能够表示晶状体折射率分布一般特征的梯度渐变模型表达式。基于收集的我国人眼实测数据的角膜面型模型和晶状体梯度渐变形式的折射率模型,为解决人眼光学系统研究中的关键问题提供了新的方案和思路。     

环境生物技术在废水除磷脱氮中的应用及进展

随着化肥、农药、洗涤剂的普遍应用，废水中的氮、磷含量增加引起水体富营养化已经成为现代废水处理一项新的研究课题，本文综述了环境生物技术在废水除磷脱氮过程中的应用及发展前景．     

离子束生物技术及其在玉米育种中的应用

离子束作为一种新兴诱变源,已广泛应用于各种农作物的诱变育种。离子束生物技术为玉米品种的改良和种质资源的创新开辟了一条新的道路,推动了玉米育种事业的长足发展。本文阐述了离子束的特点、诱变机理以及在玉米育种中的应用。     

Alternative technologies for biotechnological fuel ethanol manufacturing

The challenges of implementing biorefineries on a global scale include socioeconomic, financial, and technological constraints. In particular, the development of biorefineries is tightly linked to the continued availability of fermentation raw materials. These constraints can be relaxed by the use of diverse raw materials, while advances that confer higher flexibility would enable biotechnological plant managers to swiftly react to volatile markets. In conventional processes, Saccharomyces cerevisiae grows on a relatively limited range of substrates, and produces only a single product—ethanol. Given the observed maturity of the S. cerevisiae fermentation technology, alternatives to baker's yeast may be needed to tip the economic balance in favour of biotechnological ethanol. These alternative fermentation technologies may allow a greater diversity of substrates to be used to produce an individually tailored mix of ethanol and other chemicals. Copyright © 2007 Society of Chemical Industry     

Biochemical methane potential (BMP) of solid organic substrates: evaluation of anaerobic biodegradability using data from an international interlaboratory study

BACKGROUND: This paper describes results obtained for different participating research groups in an interlaboratory study related to biochemical methane potential (BMP). In this research work, all experimental conditions influencing the test such as inoculum, substrate characteristics and experimental conditions were investigated. The study was performed using four substrates: three positive control substrates (starch, cellulose and gelatine), and one raw biomass material (mung bean) at two different inoculum to substrate ratios (ISR). RESULTS: The average methane yields for starch, cellulose, gelatine and mung bean at ISR of 2 and 1 were 350 ± 33, 350 ± 29, 380 ± 42, 370 ± 36 and 370 ± 35 mL CH₄ g^?1 VS_added, respectively. The percentages of biotransformation of these substrates into methane were 85 ± 8, 85 ± 7, 88 ± 9, 85 ± 8 and 85 ± 8%, respectively. On the other hand, the first‐order rate constants obtained from the experimental data were 0.24 ± 0.14, 0.23 ± 0.15, 0.27 ± 0.13, 0.31 ± 0.17 and 0.23 ± 0.13 d^?1, respectively. CONCLUSION: The influence of inocula and experimental factors was nearly insignificant with respect to the extents of the anaerobic biodegradation, while the rates differed significantly according to the experimental approaches. Copyright © 2011 Society of Chemical Industry     

胶原在生物医学领域的应用

胶原是动物体中最丰富的蛋白资源，由于胶原具有很低的免疫原性，与多种宿主细胞及组织有着良好的相互作用，因而可广泛地用于制备胶原基医用材料及医用装置。本文介绍了胶原作为医用材料的生物学优势，胶原基生物材料在医疗领域的使用，以及胶原基生物材料的制备和注意事项。